Gene Cloning and DNA Analysis: An Introduction / Edition 6

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Overview

"Known world-wide as the standard introductory text to this important and exciting area, the fifth edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented two-colour illustrations." "In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology." Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students, including students of genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject.
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Product Details

  • ISBN-13: 9781444334074
  • Publisher: Wiley, John & Sons, Incorporated
  • Publication date: 4/19/2010
  • Edition number: 6
  • Pages: 336
  • Product dimensions: 7.30 (w) x 9.80 (h) x 0.90 (d)

Meet the Author

Terry Brown, University of Manchester, Faculty of Life Sciences, UK

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Table of Contents

1 Why gene cloning and DNA analysis are important 3
2 Vectors for gene cloning : plasmids and bacteriophages 14
3 Purification of DNA from living cells 28
4 Manipulation of purified DNA 54
5 Introduction of DNA into living cells 87
6 Cloning vectors for E. coli 107
7 Cloning vectors for eukaryotes 132
8 How to obtain a clone of a specific gene 158
9 The polymerase chain reaction 181
10 Studying gene location and structure 199
11 Studying gene expression and function 220
12 Studying genomes 252
13 Production of protein from cloned genes 277
14 Gene cloning and DNA analysis in medicine 302
15 Gene cloning and DNA analysis in agriculture 323
16 Gene cloning and DNA analysis in forensic science and archaeology 346
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