Gene Cloning and DNA Analysis: An Introduction / Edition 6

Hardcover (Print)
Not Available on BN.com
 

Overview

Known world-wide as the standard introductory text to this important and exciting area, the sixth edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations.

In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology.

Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves.

"… the book content is elegantly illustrated and well organized in clear-cut chapters and subsections… there is a Further Reading section after each chapter that contains several key references… What is extremely useful, almost every reference is furnished with the short but distinct author's remark."
Journal of Heredity, 2007 (on the previous edition)

Read More Show Less

Product Details

  • ISBN-13: 9781444334074
  • Publisher: Wiley, John & Sons, Incorporated
  • Publication date: 4/19/2010
  • Edition number: 6
  • Pages: 336
  • Product dimensions: 7.30 (w) x 9.80 (h) x 0.90 (d)

Meet the Author

Terry Brown, University of Manchester, Faculty of Life Sciences, UK
Read More Show Less

Table of Contents

Preface to the Sixth Edition.

Part 1: The Basic Principles of Gene Cloning and DNA Analysis.

1 Why Gene Cloning and DNA Analysis are Important.

1.1 The early development of genetics.

1.2 The advent of gene cloning and the polymerase chain reaction.

1.3 What is gene cloning?

1.4 What is PCR?

1.5 Why gene cloning and PCR are so important.

1.6 How to find your way through this book.

2 Vectors for Gene Cloning: Plasmids and Bacteriophages.

2.1 Plasmids.

2.2 Bacteriophages.

3 Purification of DNA from Living Cells.

3.1 Preparation of total cell DNA.

3.2 Preparation of plasmid DNA.

3.3 Preparation of bacteriophage DNA.

4 Manipulation of Purified DNA.

4.1 The range of DNA manipulative enzymes.

4.2 Enzymes for cutting DNA – restriction endonucleases.

4.3 Ligation – joining DNA molecules together.

5 Introduction of DNA into Living Cells.

5.1 Transformation – the uptake of DNA by bacterial cells.

5.2 Identification of recombinants.

5.3 Introduction of phage DNA into bacterial cells.

5.4 Identification of recombinant phages.

5.5 Introduction of DNA into non-bacterial cells.

6 Cloning Vectors for E. coli.

6.1 Cloning vectors based on E. coli plasmids.

6.2 Cloning vectors based on M13 bacteriophage.

6.3 Cloning vectors based on l bacteriophage.

6.4 λ and other high capacity vectors enable genomic libraries to be constructed.

6.5 Vectors for other bacteria.

7 Cloning Vectors for Eukaryotes.

7.1 Vectors for yeast and other fungi.

7.2 Cloning vectors for higher plants.

7.3 Cloning vectors for animals.

8 How to Obtain a Clone of a Specific Gene.

8.1 The problem of selection.

8.2 Direct selection.

8.3 Identification of a clone from a gene library.

8.4 Methods for clone identification.

9 The Polymerase Chain Reaction.

9.1 The polymerase chain reaction in outline.

9.2 PCR in more detail.

9.3 After the PCR: studying PCR products.

9.4 Real time PCR enables the amount of starting material to be quantified.

Part 2: The Applications of Gene Cloning and DNA Analysis in Research.

10 Sequencing Genes and Genomes.

10.1 The methodology for DNA sequencing.

10.2 How to sequence a genome.

11 Studying Gene Expression and Function.

11.1 Studying the RNA transcript of a gene.

11.2 Studying the regulation of gene expression.

11.3 Identifying and studying the translation product of a cloned gene.

12 Studying Genomes.

12.1 Genome annotation.

12.2 Studies of the transcriptome and proteome.

Part 3: The Applications of Gene Cloning and DNA Analysis in Biotechnology.

13 Production of Protein from Cloned Genes.

13.1 Special vectors for expression of foreign genes in E. coli.

13.2 General problems with the production of recombinant protein in E. coli.

13.3 Production of recombinant protein by eukaryotic cells.

14 Gene Cloning and DNA Analysis in Medicine.

14.1 Production of recombinant pharmaceuticals.

14.2 Identification of genes responsible for human diseases.

14.3 Gene therapy.

15 Gene Cloning and DNA Analysis in Agriculture.

15.1 The gene addition approach to plant genetic engineering.

15.2 Gene subtraction.

15.3 Problems with genetically modified plants.

16 Gene Cloning and DNA Analysis in Forensic Science and Archaeology.

16.1 DNA analysis in the identification of crime suspects.

16.2 Studying kinship by DNA profiling.

16.3 Sex identification by DNA analysis.

16.4 Archaeogenetics – using DNA to study human prehistory.

Glossary.

Index.

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star

(0)

4 Star

(0)

3 Star

(0)

2 Star

(0)

1 Star

(0)

Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation

Reminder:

  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

 
Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)