Gene Cloning : An Introduction / Edition 3

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More About This Textbook

Overview

The third edition of 'Gene Cloning' is a fully revised and updated version of the most popular UK introductory text on this subject. The third edition is longer and contains greater detail, yet is still aimed squarely at undergraduates and at those who have little or no previous experience of cloning techniques.

Purification of DNA from living cells/introduction of DNA into living cells/studying gene expression/PCR/etc.

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Editorial Reviews

Booknews
An introductory textbook updated to incorporate advances made since the first edition was published in 1986, but retaining its mission to serve undergraduates with no previous experience of the subject and experienced researchers new to gene cloning. Annotation c. Book News, Inc., Portland, OR (booknews.com)
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Product Details

  • ISBN-13: 9780412622403
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/13/1995
  • Edition description: 3rd ed
  • Edition number: 3
  • Pages: 334
  • Product dimensions: 7.47 (w) x 9.73 (h) x 0.70 (d)

Table of Contents

Preface
Preface to the First Edition
Preface to the Second Edition
1 Why gene cloning is important 1
2 Vehicles: plasmids and bacteriophages 13
3 Purification of DNA from living cells 27
4 Manipulation of purified DNA 52
5 Introduction of DNA into living cells 87
6 Cloning vectors for E. coli 107
7 Cloning vectors for organisms other than E. coli 133
8 How to obtain a clone of a specific gene 159
9 Studying gene and genome structure 183
10 Studying gene expression 206
11 The polymerase chain reaction 228
12 Production of protein from cloned genes 253
13 Gene cloning in medicine 274
14 Gene cloning in agriculture 295
What to read next? 312
Glossary 313
Index 327
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