Gene Delivery to Mammalian Cells: Volume 2: Viral Gene Transfer Techniques / Edition 1

Gene Delivery to Mammalian Cells: Volume 2: Viral Gene Transfer Techniques / Edition 1

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by William C. Heiser
     
 

Whether to assess the function of new genes identified from the Human Genome Project or to apply gene therapy successfully, it is often necessary to deliver genes to specific cells. In Gene Delivery to Mammalian Cells, highly experienced researchers describe in great detail methods that have proven most useful in delivering genes to mammalian cells. Volume 2:

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Overview

Whether to assess the function of new genes identified from the Human Genome Project or to apply gene therapy successfully, it is often necessary to deliver genes to specific cells. In Gene Delivery to Mammalian Cells, highly experienced researchers describe in great detail methods that have proven most useful in delivering genes to mammalian cells. Volume 2: Viral Gene Transfer Techniques details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors, adenovirus, adeno-associated viruses, alphavirus, herpes simplex virus, baculovirus, and retrovirus. Many of these techniques have only been in practice for a few years and are still being refined and updated. Some are being used not only in basic science, but also in gene therapy applications. Each prool contains step-by-step instructions, along with background notes, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Introductory chapters review the delivery methods presented, discussing their advantages and disadvantages, how they have been used successfully for gene delivery, and the future of their technology. An accompanying volume, Volume 1: Nonviral Gene Transfer Techniques, focuses on gene delivery by a variety of chemical and physical methods.

Comprehensive and cutting-edge, Gene Delivery to Mammalian Cells, volumes 1 and 2, offers biomedical specialists in industry and academia an unrivaled collection of reliable methods for the chemical, physical, and viral delivery of genes to mammalian cells, all prepared to save laboratory time and ensure experimental success.

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Product Details

ISBN-13:
9781617373534
Publisher:
Springer-Verlag New York, LLC
Publication date:
11/10/2010
Series:
Methods in Molecular Biology Series, #246
Edition description:
Softcover reprint of hardcover 1st ed. 2004
Pages:
566
Product dimensions:
9.00(w) x 6.00(h) x 1.30(d)

Table of Contents

Part I. Delivery Using Adenoviruses

Adenovirus-Mediated Gene Delivery: An Overview
Joanne T. Douglas

DNA Delivery to Cells in Culture: Generation of Adenoviral Libraries for High-Throughput Functional Screening
Miroslava Ogorelkova, Seyyed Mehdy Elahi, David Gagnon, and Bernard Massie

Adenovirus-Mediated Gene Delivery to Skeletal Muscle
Joanne T. Douglas

Delivery of Adenoviral DNA to Mouse Liver
Sheila Connelly and Christine Mech

Delivery of DNA to Lung Airway Epithelium
Daniel J. Weiss

Delivery of DNA to Pulmonary Endothelium Using Adenoviral Vectors
Paul N. Reynolds

Gene Transfer to Brain and Spinal Cord Using Recombinant Adenoviral Vectors
Joseph M. Alisky and Beverly L. Davidson.

Adenovirus-Mediated Gene Transfer to Tumor Cells
Manel Cascalló and Ramon Alemany

Adenovirus-Mediated Gene Delivery to Dendritic Cells
Laura Timares, Joanne T. Douglas, Bryan W. Tillman, Victor Krasnykh, and David T. Curiel

Part II. Delivery Using Adeno-Associated Viruses

Overview of Adeno-Associated Viral Vectors
Thomas M. Daly

AAV Vector Delivery to Cells in Culture
Andrew Smith, Roy Collaco, and James P. Trempe

AAV-Mediated Gene Transfer to Skeletal Muscle
Roland W. Herzog

AAV-Mediated Gene Transfer to the Liver
Thomas M. Daly. AAV-Mediated Gene Transfer to Mouse Lungs, Christine L. Halbert and A. Dusty Miller

Gene Delivery to the Mammalian Heart Using AAV Vectors
Danny Chu, Patricia A. Thistlethwaite, Christopher C. Sullivan, Mirta S. Grifman, and Matthew D. Weitzman

Gene Delivery to the Mouse Brain with Adeno-Associated Virus
Marco A. Passini, Deborah J. Watson, and John H. Wolfe

Delivery of DNA to Tumor Cells In Vivo Using Adeno-Associated Virus
Selvarangan Ponnazhagan and Frank Hoover

Gene Delivery toHuman and Murine Primitive Hematopoietic Stem and Progenitor Cells by AAV2 Vectors
Arun Srivastava

Part III. Delivery Using Herpes Simplex Viruses

Delivery Using Herpes Simplex Virus: An Overview
William F. Goins, Darren Wolfe, David M. Krisky, Qing Bai, Ed A. Burton, David J. Fink, and Joseph C. Glorioso

Gene Transfer to Skeletal Muscle Using Herpes Simplex Virus-Based Vectors
Baohong Cao and Johnny Huard

Delivery of Herpes Simplex Virus-Based Vectors to the Nervous System
James R. Goss, Atsushi Natsume, Darren Wolfe, Marina Mata, Joseph C. Glorioso, and David J. Fink

Gene Transfer to Glial Tumors Using Herpes Simplex Virus
Ajay Niranjan, Darren Wolfe, Wendy Fellows, William F. Goins, Joseph C. Glorioso, Douglas Kondziolka, and L. Dade Lunsford

Delivery of Herpes Simplex Virus-Based Vectors to Stem Cells
Darren Wolfe, James B. Wechuck, David M. Krisky, Julie P. Goff, William F. Goins, Ali Ozuer, Michael E. Epperly, Joel S. Greenberger, David J. Fink, and Joseph C. Glorioso

Part IV. Delivery Using Baculoviruses

Baculovirus-Mediated Gene Delivery into Mammalian Cells
Raymond V. Merrihew, Thomas A. Kost, and J. Patrick Condreay

Part V. Delivery Using Lentiviruses

Gene Delivery by Lentivirus Vectors: An Overview
Tal Kafri

Lentiviral Vectors for the Delivery of DNA into Mammalian Cells
Roland Wolkowicz, Garry P. Nolan, and Michael A. Curran

Stable Gene Delivery to CNS Cells Using Lentiviral Vectors
Deborah J. Watson, Brian A. Karolewski, and John H. Wolfe

Gene Delivery to Hematopoietic Stem Cells Using Lentiviral Vectors
Hiroyuki Miyoshi

Delivery of Genes to the Eye Using Lentiviral Vectors
Masayo Takahashi

Lentiviral Transduction of Human Dendritic Cells
Roland Schroers and Si-Yi Chen

Part VI. Delivery Using Retroviruses

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