Gene Delivery to Mammalian Cells: Volume 1: Nonviral Gene Transfer Techniques / Edition 1 by William C. Heiser | 9781617373497 | Paperback | Barnes & Noble
Gene Delivery to Mammalian Cells: Volume 1: Nonviral Gene Transfer Techniques / Edition 1

Gene Delivery to Mammalian Cells: Volume 1: Nonviral Gene Transfer Techniques / Edition 1

by William C. Heiser
     
 

ISBN-10: 1617373494

ISBN-13: 9781617373497

Pub. Date: 11/10/2010

Publisher: Springer-Verlag New York, LLC

Highly experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation,

Overview

Highly experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.

Product Details

ISBN-13:
9781617373497
Publisher:
Springer-Verlag New York, LLC
Publication date:
11/10/2010
Series:
Methods in Molecular Biology Series, #245
Edition description:
Softcover reprint of hardcover 1st ed. 2004
Pages:
301
Product dimensions:
5.98(w) x 9.02(h) x 0.03(d)

Table of Contents

Part I. Delivery Using Chemical Methods

Chemical Methods for DNA Delivery: An Overview
Dexi Liu, Evelyn F. Chiao, and Hui Tian

Gene Transfer into Mammalian Cells Using Calcium Phosphate and DEAE-Dextran
Gregory S. Pari and Yiyang Xu

DNA Delivery to Cells in Culture Using Peptides
Lei Zhang, Nicholas Ambulos, and A. James Mixson

DNA Delivery to Cells in Culture Using PNA Clamps
Todd D. Giorgio and Shelby K. Wyatt

Dendrimer-Mediated Cell Transfection In Vitro
James R. Baker, Jr., Anna U. Bielinska, and Jolanta F. Kukowska-Latallo

DNA Delivery to Cells in Culture Using Cationic Liposomes
Shelby K. Wyatt and Todd D. Giorgio

Formulation of Synthetic Gene Delivery Vectors for Transduction of the Airway Epithelium
John Marshall, Nelson S. Yew, and Seng H. Cheng

Cationic Liposome-Mediated DNA Delivery to the Lung Endothelium
Young K. Song, Guisheng Zhang, and Dexi Liu

Delivery of DNA to Tumor Cells Using Cationic Liposomes
Duen-Hwa Yan, Bill Spohn, and Mien-Chie Hung

Delivery of Transposon DNA to Lungs of Mice Using Polyethyleneimine-DNA Complexes
Lalitha R. Belur and R. Scott McIvor

Part II. Delivery Using Physical Methods

Gene Delivery Using Physical Methods: An Overview
Te-hui W. Chou, Subhabrata Biswas, and Shan Lu

Gene Delivery to Mammalian Cells by Microinjection
Robert King

Delivery of DNA to Cells in Culture Using Particle Bombardment
William C. Heiser

Delivery of DNA to Skin by Particle Bombardment
Shixia Wang, Swati Joshi, and Shan Lu

Biolistic Transfection of Cultured Organotypic Brain Slices
A. Kimberley McAllister

Efficient Electroporation of Mammalian Cells in Culture
Peter A. Barry

Delivery of DNA to Skin by Electroporation
Nathalie Dujardin and Véronique Préat

In VivoDNA Electrotransfer in Skeletal Muscle
Guenhaël Sanz, Saulius Satkauskas, and Lluis M. Mir

Electrically Mediated Plasmid DNA Delivery to Solid Tumors In Vivo
Mark J. Jaroszeski, Loree C. Heller, Richard Gilbert, and Richard Heller

Hydrodynamic Delivery of DNA
Joseph E. Knapp and Dexi Liu

Naked DNA Gene Transfer in Mammalian Cells
Guofeng Zhang, Vladimir G. Budker, James J. Ludtke, and Jon A. Wolff

Microparticle Delivery of Plasmid DNA to Mammalian Cells
Mary Lynne Hedley and Shikha P. Barman

DNA Delivery to Cells in Culture Using Ultrasound
Thomas P. McCreery, Robert H. Sweitzer, and Evan C. Unger

DNA Delivery to Cells In Vivo by Ultrasound
Thomas P. McCreery, Robert H. Sweitzer, Evan C. Unger, and Sean Sullivan

Index

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