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From The CriticsReviewer: Michael Cummings, PhD (University of Illinois at Chicago)
Description: Using a combination of recombinant DNA technology and embryonic stem cells, it is now possible to introduce literally any mutation into the germ cells of mice via homologous recombination. This technique, known as gene targeting, allows a wide range of mutations to be produced. Introduced about ten years ago, gene targeting has developed rapidly, and is widely used by molecular biologists, cell biologists, and geneticists to study gene function.
Purpose: This new edition updates existing techniques and introduces detailed protocols for newer techniques including the use of gene targeting for the production of conditional mutants.
Audience: The intended audience is molecular and cell biologists and geneticists.
Features: This is an up-to-date and comprehensive source for gene targeting protocols. In addition to the protocols, this book contains material on the design of vectors, as well as the steps involved in designing a new mutation, generating the mutant mouse and evaluating the phenotype. These and other topics are covered in seven chapters, beginning with an introduction and overview of gene targeting, and the use of knock-out and knock-in targeting vectors. Other chapters contain protocols for site-specific recombination, production of targeted stem cell clones, chimeras, the use of gene trap strategies, and the relationship between classical genetics and gene targeting.
Assessment: This book is indispensable and required reading for anyone contemplating the use of gene targeting, or anyone seeking a single source for a collection of the latest protocols. It is well-organized, clearly written, and a valuable resource for researchers.