Gene Therapy of Cancer: Methods and Protocols / Edition 2

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Overview

Despite various difficulties, the field of gene therapy, particularly with regard to cancer, has accumulated a tremendous amount of vital pre-clinical and clinical data. Gene Therapy of Cancer: Methods and Protocols Second Edition fully updates the first edition with expert coverage of established and novel protocols involving both experimental and clinical approaches to cancer-gene therapy. This state-of-the-art volume contains overviews of new concepts and strategies with chapters on regulatory and ethical issues, developments, problems and possible limitations of design and production of gene therapeutics as well as translational issues. Written in the highly successful Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Cutting edge and authoritative, Gene Therapy of Cancer: Methods and Protocols, Second Edition is an ideal guide for all those who wish to explore the fast-paced and critical study of non viral, viral, experimental and clinical cancer gene therapy.

The book contains black-and-white illustrations.

Those who enter the fast-evolving, exciting, and controversial field of cancer gene therapy need substantial protocols, methods, and helpful hints in order to add value to their research experience. This book covers a broad spectrum of current protocols for gene therapy on cancer, including cutting edge approaches to delivering therapeutic genes into tumors. Well-known researchers in the field offer a variety of protocols for different approaches in cancer gene therapy, with short introductions to each chapter that give a brief update for the method described in that chapter. The main important feature of this text is its combination of numerous experimental and clinical studies that cover a field of research which has growing clinical applications for the future.

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Editorial Reviews

Richard K. Burt
The editors describe in step-by-step fashion different genes and delivery methods to be used for gene therapy of cancer. The purpose is to provide a broad range of protocols for the delivery of therapeutic genes into patients with tumors. This is worthy in a rapidly advancing field and the editors' objectives are met. This book is written for a wide ranging audience including experts in the field of gene therapy as well as medical practitioners. The editors and contributors are credible investigators in their field who have combined not only the basic science methods of transduction, but also introductions explaining the clinical ramifications. A wide variety of methods used to deliver genes to tumors are discussed, as are the type of genes used. This includes tumor vaccination, suicide genes, antisense genes, ribozymes, etc. The unique aspect of this book is the translational approach of explaining laboratory techniques as well as the application to patients. This book is well written and a translational resource for those interested in the rapidly advancing and novel field of gene therapy for malignancies.
Booknews
Thirty-nine contributions survey a wide array of the methodologies being currently used and developed for gene therapy for cancer. Representative methods are delineated in immunotherapy and tumor vaccination, suicide gene therapy, antioncogene therapy, and antisense and ribozyme gene therapy. The first 26 papers review experimental approaches utilizing both viral and non-viral gene delivery systems, while Part II links basic research to clinical protocols. Practical guidance is provided for researchers as well as graduate students in this rapidly evolving field. The editors are affiliated with the Max-Delbr<:u>ck Center for Molecular Medicine in Berlin, Germany. Contributors are from the US, Europe, and Japan. Spiral-comb binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)
Doody's Review Service
Reviewer: Richard K. Burt, MD (Northwestern University Feinberg School of Medicine)
Description: The editors describe in step-by-step fashion different genes and delivery methods to be used for gene therapy of cancer.
Purpose: The purpose is to provide a broad range of protocols for the delivery of therapeutic genes into patients with tumors. This is worthy in a rapidly advancing field and the editors' objectives are met.
Audience: This book is written for a wide ranging audience including experts in the field of gene therapy as well as medical practitioners. The editors and contributors are credible investigators in their field who have combined not only the basic science methods of transduction, but also introductions explaining the clinical ramifications.
Features: A wide variety of methods used to deliver genes to tumors are discussed, as are the type of genes used. This includes tumor vaccination, suicide genes, antisense genes, ribozymes, etc. The unique aspect of this book is the translational approach of explaining laboratory techniques as well as the application to patients.
Assessment: This book is well written and a translational resource for those interested in the rapidly advancing and novel field of gene therapy for malignancies.

3 Stars from Doody
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Product Details

  • ISBN-13: 9781934115855
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 4/28/2009
  • Series: Methods in Molecular Biology Series , #542
  • Edition description: 2nd ed. 2009
  • Edition number: 2
  • Pages: 738
  • Product dimensions: 7.60 (w) x 10.30 (h) x 1.60 (d)

Table of Contents

Preface
Contributors
Pt. I Experimental Approaches in Cancer Gene Therapy
A Immunotherapy/Tumor Vaccination
1 Immunizing Potential of Cytokine-Transduced Tumor Cells 3
2 Particle-Mediated Gene Transfer into Dendritic Cells: A Novel Strategy for the Induction of Immune Responses against Tumor Antigens 27
3 Cancer Gene Therapy with Heat Shock Protein-65 Gene 49
4 Recombinant Vaccinia Virus MVA for Generation and Analysis of T Cell Responses Against Tumor Associated Antigens 57
B Suicide Gene Therapy
5 Selection of Cytochrome P450 Genes for Use in Prodrug Activation-Based Cancer Gene Therapy 77
6 Construction of P450-Expressing Tumor Cell Lines Using Retroviruses 85
7 In Vitro Methods for Evaluation of P450-Based Anticancer Gene Therapy 95
8 Tumor Models for Evaluation of P450 Gene Therapy In Vivo 107
C Anti-Oncogene and Suppressor Gene Therapy
9 Intracellular Single-Chain Antibodies for Gene Therapy 121
10 Combined Adenoviral Transfer of Tumor Suppressor and Cell-Cycle Genes for Tumor-Cell Apoptosis 151
D Antisense Gene Therapy
11 Inhibition of Cell Growth by Antisense Oligonucleotides Targeting the Growth-Related Protein Kinase c-raf 167
12 IGF-1 Antisense Strategies for Cancer Treatment 189
E Ribozyme Gene Therapy
13 Anti-MDR1 Ribozyme Gene Therapy 207
14 Anti-c-erbB2 Ribozyme for Gene Therapy of Breast Cancer 247
15 Anti-K-ras Ribozyme Adenoviral Vector for Gene Therapy of Non-Small Cell Lung Cancer 261
F Delivery Systems and Tumor Targeting
16 Green Fluorescent Protein Retroviral Vector: Generation of High-Titer Producer Cells and Virus Supernatant 275
17 HSV-1 Vectors for Gene Therapy of Experimental CNS Tumors 287
18 Intratumoral Injection of Naked DNA 313
19 Cationic Liposome Gene Transfer 323
20 In Vivo Particle-Mediated Gene Transfer for Cancer Therapy 331
21 Gene Targeting to Hepatomas (AFP) 345
22 Adenovirus-Mediated Targeted Gene Therapy for Breast Cancer and for Purging Hematopoietic Stem-Cell Sources 361
23 Chemotherapy-Inducible Vector for Gene Therapy of Cancer 371
G Alternative Approaches in Cancer Gene Therapy
24 Oncolytic Adenoviral Vectors 395
25 Genetically Modified Clostridium for Gene Therapy of Tumors 413
26 Tumor-Targeted Salmonella: Strain Development and Expression of the HSV-tK Effector Gene 419
Pt. II Clinical Protocols for Cancer Gene Therapy
A Immunotherapy/Tumor Vaccination
27 Ex Vivo Cytokine Gene Transfer in Melanomas by Using Particle Bombardment 439
28 Intratumoral Gene Transfer of the HLA-B7 Gene Into Colon Carcinoma Metastases 453
29 Hybrid Cell Vaccination in Patients With Metastatic Melanoma 469
B Suicide Gene Therapy
30 Retroviral Transfer of the Herpes Simplex Virus-Thymidine Kinase (HSV-tK) Gene for the Treatment of Cancer 479
31 Gene Therapy for Treatment of Brain Tumors (HSV-tK In Vivo Gene Transfer): A Case Study 499
32 Gene Therapy of Glioblastoma Multiforme with a Bicistronic Retroviral Vector Expressing Human IL-2 and HSV-tk 511
33 Intratumoral Gene Transfer of the Cytosine Deaminase Gene for the Treatment of Breast Cancer 523
C Anti-Oncogene and Suppressor Gene Therapy
34 Adenovirus-Mediated Wild-Type p53 Gene Transfer into Head and Neck Cancers 537
35 Direct DNA Injection (p53) into HCC Tumors 545
36 A Phase II Trial of Intratumoral Injection with a Selectively Replicating Adenovirus (ONYX-015) in Patients with Recurrent, Refractory Squamous Cell Carcinoma of the Head and Neck 559
D Antisense Gene Therapy
37 c-myb Antisense Oligonucleotide Therapeutics for Hematologic Malignancies 577
38 Ovarian Cancer Gene Therapy with BRCA1 - An Overview 593
39 Methods for Chemoprotection and Chemosensitization: MDR-1 For Chemoprotection Using Retroviruses to Modify Hematopoietic Cells and Cytosine Deaminase for Chemosensitization Using Adenoviral Vectors to Modify Epithelian Neoplastic Cells 609
Index 617
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