Gene Therapy of Cancer: Methods and Protocols / Edition 1

by Wolfgang Walther, Ulrike Stein

Overview

Despite various difficulties, the field of gene therapy, particularly with regard to cancer, has accumulated a tremendous amount of vital pre-clinical and clinical data. "Gene Therapy of Cancer: Methods and Prools, Second Edition" fully updates the first edition with expert coverage of established and novel prools involving both experimental and clinical approaches to cancer gene therapy. This state-of-the-art volume contains overviews of new concepts and strategies with chapters on regulatory and ethical issues, developments, problems and possible limitations of design and production of gene therapeutics as well as translational issues. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible prools, and notes on troubleshooting and avoiding known pitfalls.

Cutting edge and authoritative, "Gene Therapy of Cancer: Methods and Prools, Second Edition" is an ideal guide for all those who wish to explore the fast-paced and critical study of nonviral, viral, experimental and clinical cancer gene therapy.

Product Details

ISBN-13:	9780896038431
Publisher:	Springer-Verlag New York, LLC
Publication date:	02/17/2000
Series:	Methods in Molecular Medicine , #35
Edition description:	2000
Pages:	645
Product dimensions:	6.10(w) x 9.25(h) x 0.05(d)

About the Author

Walther, Wolfgang (Max-Delbruck Center for Molecular Medicine); Stein, Ulrike (Max-Delbruck Center for Molecular Medicine)

Table of Contents

Experimental Approaces To Cancer Gene Therapy.- Immunizing Potential of Cytokine-Transduced Tumor Cells.- Particle-Mediated Gene Transfer into Dendritic Cells.- Cancer Gene Therapy With Heat Shock Protein-65 Gene.- Recombinant Vaccinia Virus MVA for Generation and Analysis of T Cell Responses Against Tumor Associated Antigens.- Experimental Approaces To Cancer Gene Therapy.- Selection of Cyhrome P450 Genes for Use in Prodrug Activation-Based Cancer Gene Therapy.- Construction of P450-Expressing Tumor Cell Lines Using Retroviruses.- 7 In Vitro Methods for Evaluation of P450-Based Anticancer Gene Therapy.- 8 Tumor Models for Evaluation of P450 Gene Therapy In Vivo.- Experimental Approaces To Cancer Gene Therapy.- 9 Intracellular Single-Chain Antibodies for Gene Therapy.- Combined Adenoviral Transfer of Tumor Suppressor and Cell-Cycle Genes for Tumor-Cell Apoptosis.- Experimental Approaces To Cancer Gene Therapy.- Inhibition of Cell Growth by Antisense Oligonucleotides Targeting the Growth-Related Protein Kinase c-raf.- IGF-1 Antisense Strategies for Cancer Treatment.- Experimental Approaces To Cancer Gene Therapy.- Anti-MDR1 Ribozyme Gene Therapy.- Anti-c-erb1B2 Ribozyme for Gene Therapy of Breast Cancer.- Anti-K-ras1 Ribozyme Adenoviral Vector for Gene Therapy of Non-Small Cell Lung Cancer.- Experimental Approaces To Cancer Gene Therapy.- Green Fluorescent Protein Retroviral Vector.- HSV-1 Vectors for Gene Therapy of Experimental CNS Tumors.- Intratumoral Injection of Naked DNA.- Cationic Liposome Gene Transfer.- In Vivo Particle-Mediated Gene Transfer for Cancer Therapy.- Gene Targeting to Hepatomas (AFP).- Adenovirus-Mediated Targeted Gene Therapy for Breast Cancer and for Purging Hematopoietic Stem-Cell Sources.- Chemotherapy-Inducible Vector for Gene Therapy of Cancer.- Experimental Approaces To Cancer Gene Therapy.- Oncolytic Adenoviral Vectors.- Genetically Modified Clostridium for Gene Therapy of Tumors.- Tumor-Targeted Salmonella: Strain Development and Expression of the HSV-tK Effector Gene.- Clinical Prools for Cancer Gene Therapy.- Ex Vivo Cytokine Gene Transfer in Melanomas by Using Particle Bombardment.- Intratumoral Gene Transfer of the HLA-B7 Gene Into Colon Carcinoma Metastases.- Hybrid Cell Vaccination in Patients With Metastatic Melanoma.- Clinical Prools for Cancer Gene Therapy.- Retroviral Transfer of the Herpes Simplex Virus-Thymidine Kinase (HSV-tK) Gene for the Treatment of Cancer.- Gene Therapy for Treatment of Brain Tumors (HSV-tKIn Vivo Gene Transfer).- Gene Therapy of Glioblastoma Multiforme with a Bicistronic Retroviral Vector Expressing Human IL-2 and HSV-tK.- Intratumoral Gene Transfer of the Cytosine Deaminase Gene for the Treatment of Breast Cancer.- Clinical Prools for Cancer Gene Therapy.- Adenovirus-Mediated Wild-Type p53 Gene Transfer into Head and Neck Cancers.- Direct DNA Injection (p53) into HCC Tumors.- A Phase II Trial of Intratumoral Injection with a Selectively Replicating Adenovirus (ONYX-015) in Patients with Recurrent, Refractory Squamous Cell Carcinoma of the Head and Neck.- Clinical Prools for Cancer Gene Therapy.- c-myb Antisense Oligonucleotide Therapeutics for Hematologic Malignancies.- Ovarian Cancer Gene Therapy with BRCA1—An Overview.- Methods for Chemoprotection and Chemosensitization.

Editorial Reviews

Reviewer: Richard K. Burt, MD (Northwestern University Feinberg School of Medicine)
Description: The editors describe in step-by-step fashion different genes and delivery methods to be used for gene therapy of cancer.
Purpose: The purpose is to provide a broad range of protocols for the delivery of therapeutic genes into patients with tumors. This is worthy in a rapidly advancing field and the editors' objectives are met.
Audience: This book is written for a wide ranging audience including experts in the field of gene therapy as well as medical practitioners. The editors and contributors are credible investigators in their field who have combined not only the basic science methods of transduction, but also introductions explaining the clinical ramifications.
Features: A wide variety of methods used to deliver genes to tumors are discussed, as are the type of genes used. This includes tumor vaccination, suicide genes, antisense genes, ribozymes, etc. The unique aspect of this book is the translational approach of explaining laboratory techniques as well as the application to patients.
Assessment: This book is well written and a translational resource for those interested in the rapidly advancing and novel field of gene therapy for malignancies.

Doody's Review Service

3 Stars from Doody

The editors describe in step-by-step fashion different genes and delivery methods to be used for gene therapy of cancer. The purpose is to provide a broad range of protocols for the delivery of therapeutic genes into patients with tumors. This is worthy in a rapidly advancing field and the editors' objectives are met. This book is written for a wide ranging audience including experts in the field of gene therapy as well as medical practitioners. The editors and contributors are credible investigators in their field who have combined not only the basic science methods of transduction, but also introductions explaining the clinical ramifications. A wide variety of methods used to deliver genes to tumors are discussed, as are the type of genes used. This includes tumor vaccination, suicide genes, antisense genes, ribozymes, etc. The unique aspect of this book is the translational approach of explaining laboratory techniques as well as the application to patients. This book is well written and a translational resource for those interested in the rapidly advancing and novel field of gene therapy for malignancies.

Richard K. Burt

Thirty-nine contributions survey a wide array of the methodologies being currently used and developed for gene therapy for cancer. Representative methods are delineated in immunotherapy and tumor vaccination, suicide gene therapy, antioncogene therapy, and antisense and ribozyme gene therapy. The first 26 papers review experimental approaches utilizing both viral and non-viral gene delivery systems, while Part II links basic research to clinical protocols. Practical guidance is provided for researchers as well as graduate students in this rapidly evolving field. The editors are affiliated with the Max-Delbr<:u>ck Center for Molecular Medicine in Berlin, Germany. Contributors are from the US, Europe, and Japan. Spiral-comb binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Booknews

From the reviews of the second edition:

“This book, authored by a number of prominent researchers in the field … provides an overview of recent ground-breaking experimental and clinical concepts and prools in cancer gene therapy. … the editors and authors are to be commended for producing a well-organised, authoritative and succinct coverage of the vast area of gene therapy prools. The materials are relevant, easy-to-follow and well-presented with good illustrations. Without doubt it will serve as a useful resource for those interested in this field, both scientists and clinicians … .”­­­ (Han Hsi Wong and Yaohe Wang, Human Genetics, Vol. 128, July, 2010)

From the Publisher