Genomic Imprinting: Methods and Protocols

Genomic Imprinting: Methods and Protocols

by Andrew Ward
     
 

Imprinted genes, many of which generally control growth and development, frequently lose their imprints during cancer progression, a loss that then plays a substantial role in uncontrolled tumor growth. Imprint instability also appears to be a major limitation to the success of mammalian cloning experiments. In Genomic Imprinting: Methods and Prools, Andrew

Overview

Imprinted genes, many of which generally control growth and development, frequently lose their imprints during cancer progression, a loss that then plays a substantial role in uncontrolled tumor growth. Imprint instability also appears to be a major limitation to the success of mammalian cloning experiments. In Genomic Imprinting: Methods and Prools, Andrew Ward and a team of experienced researchers have brought together a collection of optimized classic and vanguard techniques for the identification and analysis of imprinted genes. The majority of prools describe molecular techniques that allow examination of gene structure or expression in an allele-specific manner. Prools are included for identifying and cloning imprinted genes, for analyzing imprinted gene expression, for the study of DNA methylation and methylation-sensitive DNA-binding proteins, and for examining chromatin structure. There are also methods for the manipulation of mouse embryos to produce monoparental embryos and embryonic stem cells, and for the generation of transgenic mice with BAC, PAC, and YAC constructs. Each technique is described in step-by-step detail to ensure successful results.

Incorporating a wealth of knowledge from leading exponents in the field, Genomic Imprinting: Methods and Prools brings together all the essential molecular, genetic, and embryological methods commonly used in today's laboratories for the identification and analysis of imprinted genes.

Editorial Reviews

From the Publisher
"...a multi-authored volume in which the methods of gene imprinting are discussed in detail in 25 chapters. The prools are very detailed with multiple schemes and figures." - Journal of Pediatric Endocrinology and Metabolism

Product Details

ISBN-13:
9781617371646
Publisher:
Springer-Verlag New York, LLC
Publication date:
11/10/2010
Series:
Methods in Molecular Biology Series, #181
Edition description:
Softcover reprint of hardcover 1st ed. 2002
Pages:
377
Product dimensions:
5.98(w) x 9.02(h) x 0.03(d)

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