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Germ Cell Protocols: Volume 1: Sperm and Oocyte Analysis / Edition 1

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For volume 1 alone:

A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Highlights include in vitro maturation and fertilization of human, porcine, and canine oocytes; the cryopreservation of sperm cells; establishment of an in vitro spermatogenesis system; visualization of sperm accessory structures; and motility assays of stallion spermatozoa. Each readily reproducible prool is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

For both volumes:

A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. The methods cover sperm and egg activation, motility, fertilization, nuclear development, nuclear cloning, the molecular characterization of specific events, and the imaging of cell structures. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning contains methods for molecular egg analysis, live egg imaging, nuclear cloning, oocyte preservation, and nuclear transfer.

Comprehensive and cutting-edge, Germ Cell Prools offers both novice and established researchers a gold-standard collection of hard-to-find methods of high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems.

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Product Details

  • ISBN-13: 9781588291219
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 3/28/2004
  • Series: Methods in Molecular Biology Series , #253
  • Edition description: 2004
  • Edition number: 1
  • Pages: 316
  • Product dimensions: 6.46 (w) x 9.28 (h) x 0.91 (d)

Table of Contents

Cryopreservation of Semen of the Salmonidae with Special Reference to Large-Scale Fertilization
Franz Lahnsteiner

Fertilization and Sperm Chemotaxis in Ascidians
Manabu Yoshida

Assays for Vertebrate Sperm Chemotaxis
Hitoshi Sugiyama, Bader Al-Anzi, Robert W. McGaughey, and Douglas E. Chandler

Stallion Spermatozoa Viability: Comparison of Flow Cytometry with Other Methods
Katrina Merkies and Mary M. Buhr

Visualization of Sperm Accessory Structures in the Mammalian Spermatids, Spermatozoa, and Zygotes by Immunofluorescence, Confocal, and Immunoelectron Microscopy
Peter Sutovsky

Localization of Zona Pellucida Receptors on Live Sperm by Fluorophore-Conjugated Solubilized Zona Pellucida Proteins
Heather R. Burkin, Ana Paula Alves-Vieira, and David J. Miller

Detection of Human Papillomavirus DNA in Sperm Using Polymerase Chain Reaction
Olufemi A. Olatunbosun, Allison M. Case, and Harry G. Deneer

Spermatogonial Stem Cells
Karim Nayernia, Manyu Li, and Wolfgang Engel

Molecular and Cellular Approaches for Establishment of an In Vitro Spermatogenesis System
Karim Nayernia and Wolfgang Engel

Acrosin Activity Assay for the Evaluation of Mammalian Sperm Acrosome Reaction
Yehudit Lax, Sara Rubinstein, and Haim Breitbart

Isolation of Sea Urchin Sperm Plasma Membranes
Kathryn J. Mengerink and Victor D. Vacquier

Detection of Centrosome Structure in Fertilized and Artificially Activated Sea Urchin Eggs Using Immunofluorescence Microscopy and Isolation of Centrosomes Followed by Structural Characterization with Field Emission Scanning Electron Microscopy
Heide Schatten and Amitabha Chakrabarti

Microscopic Techniques for Studying Sperm-Oocyte Interaction During Fertilization and Early Embryo Development
Mark W. Tengowski

Immunocyhemical Staining of the Metaphase II Spindle in Mammalian Oocytes
Steve Mullen and John K. Critser

Spindle Imaging in Living Mammalian Oocytes Using the Liquid-Crystal Polarized Light Microscope and Its Practical Use
Wei-Hua Wang, Jimmy Gill, and Cathy Boutin

In Vitro Maturation and Fertilization of Pig Oocytes
Heng-Yu Fan and Qing-Yuan Sun

In Vitro Maturation and In Vitro Fertilization of Human Oocytes
Wei-Hua Wang and Jimmy Gill

In Vitro Maturation and Fertilization of Canine Oocytes
Karine Reynaud, Marie Saint-Dizier, and Sylvie Chastant-Maillard

Detection and Measurement of Membrane-Bound Protein Tyrosine Kinases in the Zebrafish Egg
Wenjun Wu and William H. Kinsey

A Single-Cell Method for Estimation of Protein Tyrosine Kinases in the Zebrafish Egg
Elinor MacGregor and William H. Kinsey

Activity of MAPK/p90rsk During Fertilization in Mice, Rats, and Pigs
Qing-Yuan Sun and Heng-Yu Fan


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