Germ Cell Protocols: Volume 1: Sperm and Oocyte Analysis / Edition 1

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The study of germ cells has experienced enormous advances in recent years and has now entered into an explosive phase of new discoveries with the introduction of transgenic technologies and nuclear cloning. The two volumes combine techniques in a variety of different systems and covers topics including sperm and egg activation, motility, fertilization, nuclear development, nuclear cloning, molecular characterization, imaging cell structures, cryopreservation of germ cells, and other specific techniques that will advance the investigator's knowledge of germ cells. Volume 1 is focused on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. This book will be of interest to a wide range of scientists including molecular biologists, reproductive biologists, physiologists and clinicians who need a set of reliable protocols to complete their research.

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Product Details

  • ISBN-13: 9781588291219
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 3/28/2004
  • Series: Methods in Molecular Biology Series, #253
  • Edition description: 2004
  • Edition number: 1
  • Pages: 316
  • Product dimensions: 6.46 (w) x 9.28 (h) x 0.91 (d)

Table of Contents

Cryopreservation of Semen of the Salmonidae with Special Reference to Large-Scale Fertilization
Franz Lahnsteiner

Fertilization and Sperm Chemotaxis in Ascidians
Manabu Yoshida

Assays for Vertebrate Sperm Chemotaxis
Hitoshi Sugiyama, Bader Al-Anzi, Robert W. McGaughey, and Douglas E. Chandler

Stallion Spermatozoa Viability: Comparison of Flow Cytometry with Other Methods
Katrina Merkies and Mary M. Buhr

Visualization of Sperm Accessory Structures in the Mammalian Spermatids, Spermatozoa, and Zygotes by Immunofluorescence, Confocal, and Immunoelectron Microscopy
Peter Sutovsky

Localization of Zona Pellucida Receptors on Live Sperm by Fluorophore-Conjugated Solubilized Zona Pellucida Proteins
Heather R. Burkin, Ana Paula Alves-Vieira, and David J. Miller

Detection of Human Papillomavirus DNA in Sperm Using Polymerase Chain Reaction
Olufemi A. Olatunbosun, Allison M. Case, and Harry G. Deneer

Spermatogonial Stem Cells
Karim Nayernia, Manyu Li, and Wolfgang Engel

Molecular and Cellular Approaches for Establishment of an In Vitro Spermatogenesis System
Karim Nayernia and Wolfgang Engel

Acrosin Activity Assay for the Evaluation of Mammalian Sperm Acrosome Reaction
Yehudit Lax, Sara Rubinstein, and Haim Breitbart

Isolation of Sea Urchin Sperm Plasma Membranes
Kathryn J. Mengerink and Victor D. Vacquier

Detection of Centrosome Structure in Fertilized and Artificially Activated Sea Urchin Eggs Using Immunofluorescence Microscopy and Isolation of Centrosomes Followed by Structural Characterization with Field Emission Scanning Electron Microscopy
Heide Schatten and Amitabha Chakrabarti

Microscopic Techniques for Studying Sperm-Oocyte Interaction During Fertilization and Early Embryo Development
Mark W. Tengowski

Immunocyhemical Staining of the Metaphase II Spindle in Mammalian Oocytes
Steve Mullen and John K. Critser

Spindle Imaging in Living Mammalian Oocytes Using the Liquid-Crystal Polarized Light Microscope and Its Practical Use
Wei-Hua Wang, Jimmy Gill, and Cathy Boutin

In Vitro Maturation and Fertilization of Pig Oocytes
Heng-Yu Fan and Qing-Yuan Sun

In Vitro Maturation and In Vitro Fertilization of Human Oocytes
Wei-Hua Wang and Jimmy Gill

In Vitro Maturation and Fertilization of Canine Oocytes
Karine Reynaud, Marie Saint-Dizier, and Sylvie Chastant-Maillard

Detection and Measurement of Membrane-Bound Protein Tyrosine Kinases in the Zebrafish Egg
Wenjun Wu and William H. Kinsey

A Single-Cell Method for Estimation of Protein Tyrosine Kinases in the Zebrafish Egg
Elinor MacGregor and William H. Kinsey

Activity of MAPK/p90rsk During Fertilization in Mice, Rats, and Pigs
Qing-Yuan Sun and Heng-Yu Fan


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