Germ Cell Protocols: Volume 1: Sperm and Oocyte Analysis / Edition 1

Hardcover (Print)
Used and New from Other Sellers
Used and New from Other Sellers
from $20.00
Usually ships in 1-2 business days
(Save 86%)
Other sellers (Hardcover)
  • All (10) from $20.00   
  • New (6) from $58.07   
  • Used (4) from $20.00   
Close
Sort by
Page 1 of 1
Showing All
Note: Marketplace items are not eligible for any BN.com coupons and promotions
$58.07
Seller since 2008

Feedback rating:

(169)

Condition:

New — never opened or used in original packaging.

Like New — packaging may have been opened. A "Like New" item is suitable to give as a gift.

Very Good — may have minor signs of wear on packaging but item works perfectly and has no damage.

Good — item is in good condition but packaging may have signs of shelf wear/aging or torn packaging. All specific defects should be noted in the Comments section associated with each item.

Acceptable — item is in working order but may show signs of wear such as scratches or torn packaging. All specific defects should be noted in the Comments section associated with each item.

Used — An item that has been opened and may show signs of wear. All specific defects should be noted in the Comments section associated with each item.

Refurbished — A used item that has been renewed or updated and verified to be in proper working condition. Not necessarily completed by the original manufacturer.

New
1588291219 BRAND NEW NEVER USED IN STOCK 125,000+ HAPPY CUSTOMERS SHIP EVERY DAY WITH FREE TRACKING NUMBER

Ships from: fallbrook, CA

Usually ships in 1-2 business days

  • Standard, 48 States
  • Standard (AK, HI)
$60.43
Seller since 2014

Feedback rating:

(50)

Condition: New
New book in mint condition.Please allow 3-5 business days for Expedited and 6-8 business days with Standard Shipping method. We do not ship to APO/FPO.

Ships from: SUGAR LAND, TX

Usually ships in 1-2 business days

  • Standard, 48 States
  • Express, 48 States
$72.35
Seller since 2010

Feedback rating:

(55)

Condition: New
"New, ships through UPS and DHL. Excellent customer service. Satisfaction guaranteed!! "

Ships from: STERLING HEIGHTS, MI

Usually ships in 1-2 business days

  • Canadian
  • International
  • Standard, 48 States
  • Standard (AK, HI)
$87.32
Seller since 2014

Feedback rating:

(0)

Condition: New
Hardcover New 1588291219 Brand New US Edition Book in Perfect Condition. Fast Shipping with tracking number.

Ships from: Houston, TX

Usually ships in 1-2 business days

  • Canadian
  • International
  • Standard, 48 States
  • Standard (AK, HI)
  • Express, 48 States
  • Express (AK, HI)
$91.97
Seller since 2014

Feedback rating:

(0)

Condition: New
Hardcover New in new dust jacket. Brand New US edition, 3-5 days shipping!

Ships from: Sausalito, CA

Usually ships in 1-2 business days

  • Canadian
  • International
  • Standard, 48 States
  • Standard (AK, HI)
  • Express, 48 States
  • Express (AK, HI)
$119.33
Seller since 2007

Feedback rating:

(23367)

Condition: New
BRAND NEW

Ships from: Avenel, NJ

Usually ships in 1-2 business days

  • Canadian
  • International
  • Standard, 48 States
  • Standard (AK, HI)
Page 1 of 1
Showing All
Close
Sort by

Overview

For volume 1 alone:

A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Highlights include in vitro maturation and fertilization of human, porcine, and canine oocytes; the cryopreservation of sperm cells; establishment of an in vitro spermatogenesis system; visualization of sperm accessory structures; and motility assays of stallion spermatozoa. Each readily reproducible prool is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

For both volumes:

A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. The methods cover sperm and egg activation, motility, fertilization, nuclear development, nuclear cloning, the molecular characterization of specific events, and the imaging of cell structures. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning contains methods for molecular egg analysis, live egg imaging, nuclear cloning, oocyte preservation, and nuclear transfer.

Comprehensive and cutting-edge, Germ Cell Prools offers both novice and established researchers a gold-standard collection of hard-to-find methods of high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems.

Read More Show Less

Product Details

  • ISBN-13: 9781588291219
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 3/28/2004
  • Series: Methods in Molecular Biology Series , #253
  • Edition description: 2004
  • Edition number: 1
  • Pages: 336
  • Product dimensions: 6.46 (w) x 9.28 (h) x 0.91 (d)

Table of Contents

Cryopreservation of Semen of the Salmonidae with Special Reference to Large-Scale Fertilization
Franz Lahnsteiner

Fertilization and Sperm Chemotaxis in Ascidians
Manabu Yoshida

Assays for Vertebrate Sperm Chemotaxis
Hitoshi Sugiyama, Bader Al-Anzi, Robert W. McGaughey, and Douglas E. Chandler

Stallion Spermatozoa Viability: Comparison of Flow Cytometry with Other Methods
Katrina Merkies and Mary M. Buhr

Visualization of Sperm Accessory Structures in the Mammalian Spermatids, Spermatozoa, and Zygotes by Immunofluorescence, Confocal, and Immunoelectron Microscopy
Peter Sutovsky

Localization of Zona Pellucida Receptors on Live Sperm by Fluorophore-Conjugated Solubilized Zona Pellucida Proteins
Heather R. Burkin, Ana Paula Alves-Vieira, and David J. Miller

Detection of Human Papillomavirus DNA in Sperm Using Polymerase Chain Reaction
Olufemi A. Olatunbosun, Allison M. Case, and Harry G. Deneer

Spermatogonial Stem Cells
Karim Nayernia, Manyu Li, and Wolfgang Engel

Molecular and Cellular Approaches for Establishment of an In Vitro Spermatogenesis System
Karim Nayernia and Wolfgang Engel

Acrosin Activity Assay for the Evaluation of Mammalian Sperm Acrosome Reaction
Yehudit Lax, Sara Rubinstein, and Haim Breitbart

Isolation of Sea Urchin Sperm Plasma Membranes
Kathryn J. Mengerink and Victor D. Vacquier

Detection of Centrosome Structure in Fertilized and Artificially Activated Sea Urchin Eggs Using Immunofluorescence Microscopy and Isolation of Centrosomes Followed by Structural Characterization with Field Emission Scanning Electron Microscopy
Heide Schatten and Amitabha Chakrabarti

Microscopic Techniques for Studying Sperm-Oocyte Interaction During Fertilization and Early Embryo Development
Mark W. Tengowski

Immunocyhemical Staining of the Metaphase II Spindle in Mammalian Oocytes
Steve Mullen and John K. Critser

Spindle Imaging in Living Mammalian Oocytes Using the Liquid-Crystal Polarized Light Microscope and Its Practical Use
Wei-Hua Wang, Jimmy Gill, and Cathy Boutin

In Vitro Maturation and Fertilization of Pig Oocytes
Heng-Yu Fan and Qing-Yuan Sun

In Vitro Maturation and In Vitro Fertilization of Human Oocytes
Wei-Hua Wang and Jimmy Gill

In Vitro Maturation and Fertilization of Canine Oocytes
Karine Reynaud, Marie Saint-Dizier, and Sylvie Chastant-Maillard

Detection and Measurement of Membrane-Bound Protein Tyrosine Kinases in the Zebrafish Egg
Wenjun Wu and William H. Kinsey

A Single-Cell Method for Estimation of Protein Tyrosine Kinases in the Zebrafish Egg
Elinor MacGregor and William H. Kinsey

Activity of MAPK/p90rsk During Fertilization in Mice, Rats, and Pigs
Qing-Yuan Sun and Heng-Yu Fan

Index

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star

(0)

4 Star

(0)

3 Star

(0)

2 Star

(0)

1 Star

(0)

Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation

Reminder:

  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

 
Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)