Germ Cell Protocols: Volume 1: Sperm and Oocyte Analysis / Edition 1

Hardcover (Print)
Buy New
Buy New from BN.com
$134.53
Used and New from Other Sellers
Used and New from Other Sellers
from $20.00
Usually ships in 1-2 business days
(Save 86%)
Other sellers (Hardcover)
  • All (12) from $20.00   
  • New (8) from $51.59   
  • Used (4) from $20.00   

Overview

The study of germ cells has experienced enormous advances in recent years and has now entered into an explosive phase of new discoveries with the introduction of transgenic technologies and nuclear cloning. The two volumes combine techniques in a variety of different systems and covers topics including sperm and egg activation, motility, fertilization, nuclear development, nuclear cloning, molecular characterization, imaging cell structures, cryopreservation of germ cells, and other specific techniques that will advance the investigator's knowledge of germ cells. Volume 1 is focused on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. This book will be of interest to a wide range of scientists including molecular biologists, reproductive biologists, physiologists and clinicians who need a set of reliable protocols to complete their research.

Read More Show Less

Product Details

  • ISBN-13: 9781588291219
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 3/28/2004
  • Series: Methods in Molecular Biology Series , #253
  • Edition description: 2004
  • Edition number: 1
  • Pages: 316
  • Product dimensions: 6.46 (w) x 9.28 (h) x 0.91 (d)

Table of Contents

Cryopreservation of Semen of the Salmonidae with Special Reference to Large-Scale Fertilization
Franz Lahnsteiner

Fertilization and Sperm Chemotaxis in Ascidians
Manabu Yoshida

Assays for Vertebrate Sperm Chemotaxis
Hitoshi Sugiyama, Bader Al-Anzi, Robert W. McGaughey, and Douglas E. Chandler

Stallion Spermatozoa Viability: Comparison of Flow Cytometry with Other Methods
Katrina Merkies and Mary M. Buhr

Visualization of Sperm Accessory Structures in the Mammalian Spermatids, Spermatozoa, and Zygotes by Immunofluorescence, Confocal, and Immunoelectron Microscopy
Peter Sutovsky

Localization of Zona Pellucida Receptors on Live Sperm by Fluorophore-Conjugated Solubilized Zona Pellucida Proteins
Heather R. Burkin, Ana Paula Alves-Vieira, and David J. Miller

Detection of Human Papillomavirus DNA in Sperm Using Polymerase Chain Reaction
Olufemi A. Olatunbosun, Allison M. Case, and Harry G. Deneer

Spermatogonial Stem Cells
Karim Nayernia, Manyu Li, and Wolfgang Engel

Molecular and Cellular Approaches for Establishment of an In Vitro Spermatogenesis System
Karim Nayernia and Wolfgang Engel

Acrosin Activity Assay for the Evaluation of Mammalian Sperm Acrosome Reaction
Yehudit Lax, Sara Rubinstein, and Haim Breitbart

Isolation of Sea Urchin Sperm Plasma Membranes
Kathryn J. Mengerink and Victor D. Vacquier

Detection of Centrosome Structure in Fertilized and Artificially Activated Sea Urchin Eggs Using Immunofluorescence Microscopy and Isolation of Centrosomes Followed by Structural Characterization with Field Emission Scanning Electron Microscopy
Heide Schatten and Amitabha Chakrabarti

Microscopic Techniques for Studying Sperm-Oocyte Interaction During Fertilization and Early Embryo Development
Mark W. Tengowski

Immunocyhemical Staining of the Metaphase II Spindle in Mammalian Oocytes
Steve Mullen and John K. Critser

Spindle Imaging in Living Mammalian Oocytes Using the Liquid-Crystal Polarized Light Microscope and Its Practical Use
Wei-Hua Wang, Jimmy Gill, and Cathy Boutin

In Vitro Maturation and Fertilization of Pig Oocytes
Heng-Yu Fan and Qing-Yuan Sun

In Vitro Maturation and In Vitro Fertilization of Human Oocytes
Wei-Hua Wang and Jimmy Gill

In Vitro Maturation and Fertilization of Canine Oocytes
Karine Reynaud, Marie Saint-Dizier, and Sylvie Chastant-Maillard

Detection and Measurement of Membrane-Bound Protein Tyrosine Kinases in the Zebrafish Egg
Wenjun Wu and William H. Kinsey

A Single-Cell Method for Estimation of Protein Tyrosine Kinases in the Zebrafish Egg
Elinor MacGregor and William H. Kinsey

Activity of MAPK/p90rsk During Fertilization in Mice, Rats, and Pigs
Qing-Yuan Sun and Heng-Yu Fan

Index

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star

(0)

4 Star

(0)

3 Star

(0)

2 Star

(0)

1 Star

(0)

Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation

Reminder:

  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

 
Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)