Germline Stem Cells / Edition 1

Germline Stem Cells / Edition 1

by Steven X. Hou
     
 

The knowledge gained by studying germline stem cells, the source of human and animal reproduction, may find immediate application in preserving endangered wildlife, managing commercial livestock, overcoming fertility problems in humans, and treating testicular and ovary tumors. In Germline Stem Cells, leading experts explore the parameters that define germline stem

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Overview

The knowledge gained by studying germline stem cells, the source of human and animal reproduction, may find immediate application in preserving endangered wildlife, managing commercial livestock, overcoming fertility problems in humans, and treating testicular and ovary tumors. In Germline Stem Cells, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize, and maintain them. Divided into two parts, the volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells. Following the highly successful Methods in Molecular Biology series format, each chapter includes a brief introduction to the subject, a list of necessary equipment and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Germline Stem Cells is the perfect reference for scientists exploring this particular exciting and important subdivision in the fast-paced field of stem cell research.

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Product Details

ISBN-13:
9781603272131
Publisher:
Springer-Verlag New York, LLC
Publication date:
03/28/2008
Series:
Methods in Molecular Biology Series, #450
Edition description:
2008
Pages:
273
Product dimensions:
6.20(w) x 9.30(h) x 0.90(d)

Table of Contents

Preface     v
Contributors     ix
Identification and Regulation of Germline Stem Cells in Model Organisms
The Development of Germline Stem Cells in Drosophila   David A. Dansereau   Paul Lasko     3
Analysis of the C. elegans Germline Stem Cell Region   Sarah L. Crittenden   Judith Kimble     27
Immunohistological Techniques for Studying the Drosophila Male Germline Stem Cell   Shree Ram Singh   Steven X. Hou     45
Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells   Wei Liu   Steven X. Hou     61
Structural Polarity and Dynamics of Male Germline Stem Cells in an Insect (Milkweed Bug Oncopeltus fasciatus)   David C. Dorn   August Dorn     71
High-Resolution Light Microscopic Characterization of Spermatogonia   Helio Chiarini-Garcia   Marvin L. Meistrich     95
Identification and Characterization of Spermatogonial Subtypes and Their Expansion in Whole Mounts and Tissue Sections from Primate Testes   Jens Ehmcke   Stefan Schlatt     109
Epigenetic Control in Male Germ Cells: A Transillumination-Assisted Microdissection Method for the Analysis of Developmentally Regulated Events   Durga Prasad Mishra   Paolo Sassone-Corsi     119
GDNF Maintains Mouse Spermatogonial Stem Cells In Vivo and In Vitro   Hannu Sariola   Tiina Immonen     127
In Vitro Culture and Applications of Germline Stem Cells
Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts   Ina Dobrinski   Rahul Rathi     139
Spermatogonial Stem Cell Transplantation, Testicular Function, and Restoration of Male Fertility in Mice   Derek J. McLean     149
Isolating Highly Pure Rat Spermatogonial Stem Cells in Culture   F. Kent Hamra   Karen M. Chapman   Zhuoru Wu   David L. Garbers     163
Deriving Mouse Spermatogonial Stem Cell Lines   Ilaria Falciatori   Kate Lillard-Wetherell   Zhuoru Wu   F. Kent Hamra   David L. Garbers     181
Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes   Christina Tenenhaus Dann   David L. Garbers     193
Testicular Germ Cell Tumors in Mice: New Ways to Study a Genetically Complex Trait   Jason D. Heaney   Joseph H. Nadeau     211
Study Origin of Germ Cells and Formation of New Primary Follicles in Adult Human and Rat Ovaries   Antonin Bukovsky   Satish K. Gupta   Irma Virant-Klun   Nirmala B. Upadhyaya   Pleas Copas   Stuart E. Van Meter   Marta Svetlikova   Maria E. Ayala   Roberto Dominguez     233
Index     267

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