Germline Stem Cells / Edition 1

Hardcover (Print)
Buy New
Buy New from
Used and New from Other Sellers
Used and New from Other Sellers
from $66.66
Usually ships in 1-2 business days
(Save 48%)
Other sellers (Hardcover)
  • All (12) from $66.66   
  • New (10) from $66.66   
  • Used (2) from $79.75   


The knowledge gained by studying germline stem cells, the source of human and animal reproduction, may find immediate application in preserving endangered wildlife, managing commercial livestock, overcoming fertility problems in humans, and treating testicular and ovary tumors. In Germline Stem Cells, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize, and maintain them. Divided into two parts, the volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells. Following the highly successful Methods in Molecular Biology series format, each chapter includes a brief introduction to the subject, a list of necessary equipment and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Germline Stem Cells is the perfect reference for scientists exploring this particular exciting and important subdivision in the fast-paced field of stem cell research.

Read More Show Less

Editorial Reviews

Doody's Review Service
Reviewer: Bruce A. Fenderson, PhD (Thomas Jefferson University)
Description: Stem cells of the germ lineage give rise to gonocytes and gametes. Recently, stem cells have been isolated from the adult testis. This book provides background information and protocols for identifying, isolating, characterizing, and manipulating these germline stem cells. Its 16 chapters cover topics ranging from germline stem cells in model organisms to methods for culturing and manipulating mammalian spermatogonial stem cells. The primary focus is on cell/developmental biology, biochemistry, and molecular biology.
Purpose: According to the editors, the purpose is to "present selected genetic, molecular, and cellular techniques used in germline stem cell research" with the hope that the book will stimulate basic and applied research. They write, "Knowledge gained from studying germline stem cells may find immediate application in preserving endangered wildlife, managing commercial livestock, overcoming fertility problems in humans, and treating testicular and ovarian tumors."
Audience: This book is written for basic science and clinical researchers interested in stem cell biology, as well as those interested in developmental and reproductive biology. Biomedical scientists and clinicians interested in fertility/infertility and regenerative medicine will also appreciate the wealth of new information. It may serve as a textbook for advanced students taking cell and developmental biology laboratory courses.
Features: The authors discuss current progress in identifying and culturing germline stem cells. The book is organized into two major sections: identification and regulation of germline stem cells in model organisms and culture and applications of germline stem cells. Each chapter includes essential background and procedures filled with critical details. The notes section helps explain pitfalls and highlight critical laboratory steps. The chapters include lists of equipment needed, sources for reagents, recipes, and references for additional information. Several of the figures are reproduced in full color. The authors include useful lists of markers, genes, and signaling mechanisms. The book provides a snapshot of research approaches and applications involving germline stem cells.
Assessment: This is an outstanding contribution to the literature in germ cell biology. The authors highlight their current research and provide protocols for cutting-edge research. The book is written in a style that makes difficult concepts accessible to experts and nonexperts alike. The topic is fascinating and clinically relevant and this carefully edited collection of protocols will be valued by all those interested in development of the germ lineage and stem cell biology.
Read More Show Less

Product Details

  • ISBN-13: 9781603272131
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 4/28/2008
  • Series: Methods in Molecular Biology Series , #450
  • Edition description: 2008
  • Edition number: 1
  • Pages: 273
  • Product dimensions: 6.20 (w) x 9.30 (h) x 0.90 (d)

Table of Contents

Preface     v
Contributors     ix
Identification and Regulation of Germline Stem Cells in Model Organisms
The Development of Germline Stem Cells in Drosophila   David A. Dansereau   Paul Lasko     3
Analysis of the C. elegans Germline Stem Cell Region   Sarah L. Crittenden   Judith Kimble     27
Immunohistological Techniques for Studying the Drosophila Male Germline Stem Cell   Shree Ram Singh   Steven X. Hou     45
Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells   Wei Liu   Steven X. Hou     61
Structural Polarity and Dynamics of Male Germline Stem Cells in an Insect (Milkweed Bug Oncopeltus fasciatus)   David C. Dorn   August Dorn     71
High-Resolution Light Microscopic Characterization of Spermatogonia   Helio Chiarini-Garcia   Marvin L. Meistrich     95
Identification and Characterization of Spermatogonial Subtypes and Their Expansion in Whole Mounts and Tissue Sections from Primate Testes   Jens Ehmcke   Stefan Schlatt     109
Epigenetic Control in Male Germ Cells: A Transillumination-Assisted Microdissection Method for the Analysis of Developmentally Regulated Events   Durga Prasad Mishra   Paolo Sassone-Corsi     119
GDNF Maintains Mouse Spermatogonial Stem Cells In Vivo and In Vitro   Hannu Sariola   Tiina Immonen     127
In Vitro Culture and Applications of Germline Stem Cells
Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts   Ina Dobrinski   Rahul Rathi     139
Spermatogonial Stem Cell Transplantation, Testicular Function, and Restoration of Male Fertility in Mice   Derek J. McLean     149
Isolating Highly Pure Rat Spermatogonial Stem Cells in Culture   F. Kent Hamra   Karen M. Chapman   Zhuoru Wu   David L. Garbers     163
Deriving Mouse Spermatogonial Stem Cell Lines   Ilaria Falciatori   Kate Lillard-Wetherell   Zhuoru Wu   F. Kent Hamra   David L. Garbers     181
Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes   Christina Tenenhaus Dann   David L. Garbers     193
Testicular Germ Cell Tumors in Mice: New Ways to Study a Genetically Complex Trait   Jason D. Heaney   Joseph H. Nadeau     211
Study Origin of Germ Cells and Formation of New Primary Follicles in Adult Human and Rat Ovaries   Antonin Bukovsky   Satish K. Gupta   Irma Virant-Klun   Nirmala B. Upadhyaya   Pleas Copas   Stuart E. Van Meter   Marta Svetlikova   Maria E. Ayala   Roberto Dominguez     233
Index     267

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Terms of Use.
  • - Barnes & reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously
Sort by: Showing 1 Customer Reviews
  • Anonymous

    Posted April 10, 2008

    germline stem cell protocol book

    Well organised and informative book from model organisms to human. Easy to follow the detail protocol written by top leaders in germline stem cell research. Very useful for the researcher wants to start the work in stem cell field.

    Was this review helpful? Yes  No   Report this review
Sort by: Showing 1 Customer Reviews

If you find inappropriate content, please report it to Barnes & Noble
Why is this product inappropriate?
Comments (optional)