Glycosyl Hydrolases in Biomass Conversion / Edition 1

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This book provides an up-to-date review of hydrolase structure and function relationships and the means by which these important enzymes can be better utilized. There is a focus on understanding cellulase and hemicellulase action - a strategy based in macromolecular modeling, site-directed mutagenesis, active site mapping, heterologous expression, and classical kinetic characterization. Discussions of new information about cellulase enzymes and systems highlight recent accomplishments and trends in the field of glycosyl hydrolase biochemistry, such as several new crystallographic structures of important cellulase and methods for improving activity by protein engineers.
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Product Details

  • ISBN-13: 9780841236813
  • Publisher: American Chemical Society
  • Publication date: 11/28/2000
  • Series: ACS Symposium Series , #769
  • Edition number: 1
  • Pages: 304
  • Product dimensions: 5.90 (w) x 8.80 (h) x 0.70 (d)

Table of Contents

Preface ix
1. The Road to Bioethanol: A Strategic Perspective of the U.S. Department of Energy's National Ethanol Program 2
Structure-Function Studies of Glycosyl Hydrolases
2. Role of Four Conserved Active-Site Aspartic Acid Residues in Thermobifida fusca Endoglucanase Cel6A 28
3. Gene Structure of a Bifunctional Cellulase Gene (celA) Isolated from Teredinobacter turnerae 39
4. Production of Microbial Cellulases in Transgenic Crop Plants 55
5. Hydrolysis of Mannans by Cellulomons fimi 91
Cellulase Action and Synergism
6. An Overview of Factors Influencing the Enzymatic Hydrolysis of Lignocellulosic Feedstocks 100
7. Molecular Mechanics Studies of Cellulases 112
8. Adsorption of Endoglucanase I and Cellobiohydrolase I of Trichoderma reesei during Hydrolysis of Microcrystalline Cellulose 131
9. Assessing the Efficacy of Cellulase Enzyme Preparations under Simultaneous Saccharification and Fermentation Processing Conditions 144
Enzymes for Industry
10. Phage Display of Cellulose Binding Domains for Biotechnological Application 168
11. Solid-State Enzymes for Fiber Hydrolysis 190
12. Thermostable Cellulase and Xylanase from Thermoascus aurantiacus and Their Potential Industrial Applications 204
13. Two Novel Alkalotolerant Dextranases from Streptomyces anulatus 222
14. Microbial Mannanases: Substrates, Production, and Applications 236
Author Index 265
Subject Index 267
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