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Green Fluorescent Protein: Properties, Applications and Protocols / Edition 2
     

Green Fluorescent Protein: Properties, Applications and Protocols / Edition 2

by Martin Chalfie, Steven Kain, Steven R. Kain
 

ISBN-10: 0471736821

ISBN-13: 9780471736820

Pub. Date: 10/05/2005

Publisher: Wiley

The latest edition of the definitive text in the field of fluorescent proteins

Green fluorescent protein (GFP), a biological marker cloned from jellyfish, has emerged from virtual obscurity to the forefront of biomolecular research in just a few years. GFP and related fluorescent proteins (FPs) have been intensely studied—the subjects of hundreds of

Overview

The latest edition of the definitive text in the field of fluorescent proteins

Green fluorescent protein (GFP), a biological marker cloned from jellyfish, has emerged from virtual obscurity to the forefront of biomolecular research in just a few years. GFP and related fluorescent proteins (FPs) have been intensely studied—the subjects of hundreds of in vivo projects worldwide. These studies have resulted in new applications and published articles, emerging at a breathtaking rate. The success of FPs and their many uses promise to make them some of the most powerful tools in biotechnology, pharmaceutical science, and molecular and cellular biology.

Written by pioneers in the field, this text tackles both theory and practice, offering numerous case studies, examples, illustrations, and troubleshooting tips. It examines how FPs are tailored for specific systems and used to maximize expression, and how variants are generated with altered properties. The text also explores new ways to use FPs and methods for enhancing detection in a variety of organisms and cell types.

This updated and expanded edition places emphasis on the rise of FPs and their applications in industry and biosensor research. Green Fluorescent Protein: Properties, Applications, and Protocols, Second Edition provides: New chapters on FPs, biosensors, and advances in the use of FPs in the biotechnology and pharmaceutical industries Detailed information about protocols using FPs Examples from recent notable studies Background on the biochemical and physical properties, three-dimensional structure, and molecular biology and mutation of FPs The application of FPs across organisms

Green Fluorescent Protein: Properties, Applications, and Protocols, Second Edition is essential reading for researchers and graduate students in molecular and cellular biology, physiology, biochemistry, developmental biology, neuroscience, ecology, in vivo biology, and plant molecular biology.

Product Details

ISBN-13:
9780471736820
Publisher:
Wiley
Publication date:
10/05/2005
Series:
Methods of Biochemical Analysis Series , #47
Edition description:
2nd Edition
Pages:
488
Product dimensions:
7.30(w) x 10.04(h) x 1.14(d)

Table of Contents

BACKGROUND.
The Discovery of Green Fluorescent Protein.
Photons for Reporting Molecular Events: Green Fluorescent Protein and Four Luciferase Systems.
BIOCHEMISTRY AND MOLECULAR BIOLOGY OF GREEN FLUORESCENT PROTEIN.
Biochemical and Physical Properties of Green Fluorescent Protein.
The Three-Dimensional Structure of Green Fluorescent Protein and Its Implications for Function and Design.
Molecular Biology and Mutation of Green Fluorescent Protein.
APPLICATIONS OF GREEN FLUORESCENT PROTEIN.
The Uses of Green Fluorescent Protein in Caenorhabditis elegans.
The Uses of Green Fluorescent Protein in Prokaryotes.
The Uses of Green Fluorescent Protein in Yeasts.
The Uses of Green Fluorescent Protein in Drosophila.
The Uses of Green Fluorescent Protein in Plants.
The Uses of Green Fluorescent Protein in Trangenic Vertebrates.
The Uses of Green Fluorescent Protein in Mammalian Cells.
METHODS AND PROTOCOLS.
Expressioin of Green Fluorescent Protein.
Specimen Preparation.
Visualization of Green Fluorescent Protein.
Documenting Green Fluorescent Protein.
Appendix.
Index.

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