Handbook of Fluorescence Spectroscopy and Imaging: From Ensemble to Single Molecules / Edition 1

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Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.

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Editorial Reviews

From the Publisher
"Therefore, this book represents a valuable source of informationfor both specialists in single molecule fluorescence spectroscopy and researchers new to this branch offluorescence techniques." (Anal Bioanal Chem, 12 February 2012)
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Product Details

  • ISBN-13: 9783527316694
  • Publisher: Wiley
  • Publication date: 3/29/2011
  • Edition number: 1
  • Pages: 290
  • Product dimensions: 6.90 (w) x 9.70 (h) x 0.80 (d)

Meet the Author

Markus Sauer studied chemistry in Karlsruhe, Saarbr├╝cken, andHeidelberg, and finished his PhD in Physical Chemistry at theUniversity of Heidelberg under the guidance of Prof. J├╝rgenWolfrum. After a short-term visit at LBNL, Berkeley in the group ofProf. Shimon Weiss, he was decorated with the BioFuture award toperform independent research on single-molecule handling,detection, and identification. He finished his habilitation inHeidelberg at the Institute of Physical Chemistry and is nowProfessor for Applied Laser Physics and Laser Spectroscopy at theUniversity in Bielefeld.

Johan Hofkens received his MSc. (1988) and Ph.D. degree (1993) inChemistry from the University of Leuven (K.U.Leuven). Afterpostdoctoral research with Prof. Masuhara at Osaka University andProf. Barbara at the University of Minneapolis, he rejoined theK.U.Leuven supervising the Single Molecule Unit in the group ofProf. De Schryver. In 2005 he was appointed Research Professor atthe K.U.Leuven and recently he was promoted to full professor. Hisresearch interests are fast spectroscopy, (single molecule)fluorescence microscopy and nanoscopy and the application of thesetechniques in material science and biosciences.

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Table of Contents

Basic Principles of fluorescence spectroscopy
Fluorophores and Fluorescent Labels
Fluorophore Labeling for Single-Molecule Fluorescence Spectroscopy(SMFS)
Fluorophore Selection for Single-Molecule Fluorescence Spectroscopy(SMFS) and Photobleaching Pathways
Fluorescence Correlation Spectroscopy
Excited state energy transfer
Photoinduced Electron Transfer (PET) Reactions
Super-Resolution Fluorescence Imaging
Single-molecule enzymatics

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