Handbook of Molecular and Cellular Methods in Biology and Medicine

Overview

Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method.

This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a ...

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Overview

Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method.

This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies.

The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!

The book contains black-and-white illustrations.

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Editorial Reviews

Booknews
A handbook that gathers together the widely dispersed techniques and methods in current cellular and molecular biology. Each of the 21 chapters covers the principles behind the various methods and techniques, detailed step-by-step description of each protocol, and applications of protocols to different systems. Many chapters include a troubleshooting guide. Plastic comb-binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)
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Product Details

  • ISBN-13: 9780849325113
  • Publisher: CRC Press
  • Publication date: 5/15/1995
  • Edition description: Older Edition
  • Edition number: 1
  • Pages: 496
  • Product dimensions: 7.50 (w) x 10.01 (h) x 1.04 (d)

Table of Contents

Ch. 1 Isolation and Purification of DNA 1
Ch. 2 Isolation and Purification of RNA 27
Ch. 3 Extraction and Purification of Proteins/Enzymes 41
Ch. 4 Preparation of Nucleic Acid Probes 65
Ch. 5 Electrophoresis, Blotting, and Hybridization 87
Ch. 6 cDNA Libraries 123
Ch. 7 Genomic DNA Libraries 165
Ch. 8 DNA Sequencing 211
Ch. 9 PCR Techniques and Applications 243
Ch. 10 DNA Fingerprinting 263
Ch. 11 DNA Mutagenesis In Vitro 273
Ch. 12 Inhibition of Gene Expression by Antisense DNA and RNA 289
Ch. 13 DNA Footprinting and Gel Retardation Assay 305
Ch. 14 Translation of mRNA(s) and Analysis of Proteins by Gel Electrophoresis 317
Ch. 15 Gene Transfer and Expression in Animals 329
Ch. 16 Gene Transfer and Expression in Plants 367
Ch. 17 Plant Cell Culture 395
Ch. 18 Plant Tissue Culture 401
Ch. 19 Microscopy: LM, SEM, TEM, Confocal, and Applications 409
Ch. 20 Bioseparation Techniques and Their Applications 435
Ch. 21 Preparation of Monoclonal and Polyclonal Antibodies Against Specific Protein(s) 455
Index 471
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