Herpes Simplex Virus: Methods and Protocols

Overview

Herpes Simplex Virus: Methods and Prools provides a wide collection of prools employed in various levels of herpes virus research, including basic prools on growing viruses in cell culture and cloning, manipulating and preparing viral DNA. Other chapters describe approaches to design and apply HSV-1 vectors for vaccination, cancer and gene therapy or to study specific aspects of HSV-1 biology such as latency, intracellular transport and protein-protein interaction. Procedures for structural analyses, microscopy, ...

See more details below
Hardcover (2014)
$139.00
BN.com price
Other sellers (Hardcover)
  • All (8) from $107.70   
  • New (6) from $107.70   
  • Used (2) from $138.99   
Sending request ...

Overview

Herpes Simplex Virus: Methods and Prools provides a wide collection of prools employed in various levels of herpes virus research, including basic prools on growing viruses in cell culture and cloning, manipulating and preparing viral DNA. Other chapters describe approaches to design and apply HSV-1 vectors for vaccination, cancer and gene therapy or to study specific aspects of HSV-1 biology such as latency, intracellular transport and protein-protein interaction. Procedures for structural analyses, microscopy, proteomics and testing of antivirals are included as well. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Herpes Simplex Virus: Methods and Prools will aid new researchers in the field of herpes virology as well as those experienced investigators wishing to embark on new techniques.

Read More Show Less

Product Details

  • ISBN-13: 9781493904273
  • Publisher: Springer New York
  • Publication date: 4/30/2014
  • Series: Methods in Molecular Biology Series , #1144
  • Edition description: 2014
  • Pages: 331
  • Product dimensions: 6.90 (w) x 10.10 (h) x 1.00 (d)

Table of Contents

1. HSV-1 Biology and Life Cycle

Roger D. Everett

2. Herpes Simplex Virus Growth, Preparation, and Assay

Peggy Marconi and Roberto Manservigi

3. Isolation of Herpes Simplex Virus Nucleocapsid DNA

Moriah Szpara

4. Construction and Characterization of Bacterial Artificial Chromosomes (BACs) Containing Herpes Simplex Virus Full-Length Genomes

Claus-Henning Nagel, Anja Pohlmann, and Beate Sodeik

5. Engineering HSV-1 Vectors for Gene Therapy

William F. Goins, Shaohua Huang, Justus B. Cohen, and Joseph C. Glorioso

6. Herpes Simplex Virus Type 1 (HSV-1)-Derived Amplicon Vectors

Matias E. Melendez, Cornel Fraefel, and Alberto L. Epstein

7. HSV-1 Amplicon Vectors as Genetic Vaccines

Andrea S. Laimbacher and Cornel Fraefel

8. Modification of HSV-1 to an Oncolytic Virus

Hiroshi Nakashima and E. Antonio Chiocca

9. Herpes Simplex Virus Mutant Generation and Dual Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo

Nancy M. Sawtell and Richard L. Thompson

10. Phenotypic and Genotypic Testing of HSV-1 Resistance to Antivirals

Andreas Sauerbrei and Kathrin Bohn

11. Using Homogeneous Primary Neuron Cultures to Study Fundamental Aspects of HSV-1 Latency and Reactivation

Ju Youn Kim, Lora A. Shiflett, Jessica A. Linderman, Ian Mohr, and Angus C. Wilson

12. Characterization of Extracellular HSV-1 Virions by Proteomics

Roger Lippé

13. A Precipitation-Based Assay to Analyze Interactions of Viral Particles with Cytosolic Host Factors

Kerstin Radtke, Fenja Anderson, and Beate Sodeik

14. Affinity Purification Combined with Mass Spectrometry to Identify Herpes Simplex Virus Protein-Protein Interactions

David G. Meckes, Jr.

15. Preparation of Herpes Simplex Virus Infected Primary Neurons for Transmission Electron Microscopy

Monica Miranda-Saksena, Ross Boadle, and Anthony L. Cunningham

16. Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication

Michael Seyffert, Anna Paula de Oliveira, Cornel Fraefel, and Rebecca Vogel

17. Expression, Purification, and Crystallization of HSV-1 Glycoproteins for Structure Determination

Samuel D. Stampfer and Ekaterina E. Heldwein

18. Cryo-EM Techniques to Resolve the Structure of HSV-1 Capsid-Associated Components

Ryan H. Rochat, Corey W. Hecksel and Wah Chiu

19. In Vivo HSV-1 DNA Transport Studies Using Murine Retinal Ganglion Cells

Jolene M. Draper, Graham S. Stephenson and Jennifer H. LaVail

20. HSV-1 Protein Expression Using Recombinant Baculoviruses

Lorry M. Grady, Ping Bai and Sandra K. Weller

21. The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines

Joshua O. Marshak, Lichun Dong, and David M. Koelle

Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star

(0)

4 Star

(0)

3 Star

(0)

2 Star

(0)

1 Star

(0)

Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation

Reminder:

  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

 
Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)