High Content Screening / Edition 1

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High content screening (HCS) was developed by Cellomics Inc. in the mid-1990s to address the need for a platform that could be used in the discovery-driven research and development required to understand the functions of genes and gene products at the level of the cell. High Content Screening: A Powerful Approach to Systems Cell Biology and Drug Discovery discusses its use as a high throughput platform to understand the functions of genes, RNA, proteins, and other cellular constituents at the level of the living cell.

High Content Screening is assembled to assist both existing users of HCS, as well as investigators considering the addition of a discovery-driven platform to their research and development activities. The chapters have been organized into sections that highlight the importance of integrating instrumentation, application software, reagents, and informatics. In addition, there is a combination of pure review chapters on key topics and specific methods chapters.

Written by a team of world leaders in the field, High Content Screening is essential reading for senior scientists, advanced lab technicians, as well as students/post-docs who wish to apply HCS in their research and development programs.

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Editorial Reviews

Doody's Review Service
Reviewer: Scott J Weir, PharmD, PhD (University of Kansas Medical Center)
Description: This book describes the evolution and current state of high content screening (HCS).
Purpose: The purpose is to describe the history of HCS; the instrumentation, software applications and sample preparation involved in HCS; markers employed (they refer to reagents); bioinformatics practices and requirements; and examples of applications in cell biology and drug discovery. This book is applicable to current cell biology and drug discovery research. With the availability of siRNA libraries for commercial purchase by universities and industry, this book is very timely.
Audience: It is intended for researchers currently working in HCS or considering HCS as a research tool, whether in academia or industry. The authors, as a rule, are very credible. It is clear that care was taken to select authors who were, or are currently, very active in the field of HCS.
Features: The chapter by Dr Taylor on the history of HCS is very helpful. I particularly liked the sections addressing bioinformatics needs and the application examples. It would be wise, in my opinion, to plan on a second edition perhaps in three years, due to the rapidly changing HCS field.
Assessment: This is one of the better books I have reviewed. As someone who works in drug discovery and early drug development, I found the book very useful.
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Product Details

  • ISBN-13: 9781588297310
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 8/15/2006
  • Series: Methods in Molecular Biology Series, #356
  • Edition description: 2006
  • Edition number: 1
  • Pages: 464
  • Product dimensions: 10.00 (w) x 7.00 (h) x 1.00 (d)

Table of Contents

Part I. Introduction to High Content Screening

Past, Present, and Future of High Content Screening and the Field of Cellomics D. Lansing Taylor

A Pharmaceutical Company User's Perspective on the Potential of High Content Screening in Drug Discovery Ann F. Hoffman and Ralph J. Garippa

Linking Microscopy and High Content Screening in Large-Scale Biomedical Research James G. Evans and Paul Matsudaira.

Part II. Instrumentation, Biological Application Software, and Sample Preparation

Requirements, Features, and Performance of High Content Screening Platforms Albert H. Gough and Paul A. Johnston

Characteristics and Value of Directed Algorithms in High Content Screening Richik N. Ghosh, Oleg Lapets, and Jeffrey R. Haskins

Characteristics and Value of Machine Learning for Imaging in High Content Screening Juergen A. Klenk

Tools for Quantitative and Validated Measurements of Cells Anne L. Plant, John T. Elliott, Alessandro Tona, Dennis McDaniel, and Kurt J. Langenbach

Automated Cell Plating and Sample Treatments for Fixed Cells in High Content Assays Gillian R. Richards, Julie E. Kerby, Grace K. Y. Chan, and Peter B. Simpson

Differentiating Primary Human Cells in Rapid-Throughput Discovery Applications Daniel R. Marshak and Dale E. Greenwalt

Use of the CellCard™ System for Analyzing Multiple Cell Types in Parallel Oren Beske, Daniel Bassoni, and Simon Goldbard

Part III. Reagents

Reagents to Measure and Manipulate Cell Functions Kenneth A. Giuliano, D. Lansing Taylor, and Alan S. Waggoner

Fluorescent Proteins and Engineered Cell Lines Nick Thomas

Optimizing the Integration of Immunoreagents and Fluorescent Probes for Multiplexed High Content Screening Assays Kenneth A. Giuliano

The HaloTag™: A Novel Technology for Cell Imaging and Protein Analysis Georgyi V. Los and Keith Wood

Protein Labeling With FlAsH and ReAsH Thomas Machleidt, Matt Robers, and George T. Hanson

Exploiting Network Biology to Improve Drug Discovery Marnie L. MacDonald and John K. Westwick

Physiological Indicators of Cell Function Michael J. Ignatius and Jeffrey T. Hung

The Use of siRNA to Validate Immunofluorescence Studies K. Gregory Moore, Wayne Speckmann, and Ronald P. Herzig

Caged Substrates Applied to High Content Screening: An Introduction With an Eye to the Future Peter G. Conrad, II, Rajesh V. Chavli, and Richard S. Givens

Part IV. Informatics and Bioinformatics

Overview of Informatics for High Content Screening R. Terry Dunlay, Wallace J. Czekalski, and Mark A. Collins

Large-Scale Data Management for High Content Screening Leon S. Garfinkel

An Integrated Biomedical Knowledge Extraction and Analysis Platform: Using Federated Search and Document Clustering Technology Donald P. Taylor

Visualization of High Content Screening Data Matthew J. Anstett

Pathway Mapping Tools for Analysis of High Content Data Sean Ekins, Yuri Nikolsky, Andrej Bugrim, Eugene Kirillov, and Tatiana Nikolskaya

Part V. Assays and Applications of High Content Screening

Systems Biology in Cancer Research: Genomics to Cellomics Jackie L. Stilwell, Yinghui Guan, Richard M. Neve, and Joe W. Gray

Target Validation in Drug Discovery Robert A. Blake

High Content Screening as a Screening Tool in Drug Discovery Anthony Nichols

Discovery of Protein Kinase Phosphatase Inhibitors Andreas Vogt and John S. Lazo

High Content Translocation Assays for Pathway Profiling Frosty Loechel, Sara Bjørn, Viggo Linde, Morten Præstegaard, and Len Pagliaro

In Vitro Cytotoxicity Assessment Peter O'Brien and Jeffrey Haskins

Neurite Outgrowth in Retinal Ganglion Cell Culture John B. Kerrison and Donald J. Zack


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