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High Pressure Effects in Molecular Biophysics and Enzymology is designed to acquaint biochemists, biophysicists, and graduate students with advances in the application of high pressure in connection with spectroscopy as a research tool in the study of biomolecules. The 23 chapters written by leading authorities present an overview of current approaches to the use of high pressure in research on enzyme kinetics, protein folding and structure, lipid bilayer structure and organization, lipid-protein interaction, and DNA structure. This important, timely volume is the first devoted exclusively to high-pressure effects in biochemistry and will be the definitive reference in its subject for the next several years.
1. Resolution of the Ambiguity of van't Hoff Plots by the Effect of Pressure on the Equilibrum, Gregorio Weber
2. Pressure-Tuning Spectroscopy: A Tool for Investigating Molecular Interactions, H. G. Drickamer
3. Use of Partial Molar Volumes of Model Compounds in the Interpretation of High-Pressure Effects on Proteins, Kenneth E. Prehoda and John L. Markley
4. Pressure-Tuning Spectroscopy of Proteins:Fourier Transform Infrared Studies in the Diamond Anvil Cell, Karel Heremans, Koen Goossens, and László Smeller
5. Temerature- and Pressure-Induced Unfolding of a Mutant of Staphylococcal Nuclease A, Maurice R. Eftink and Glen D. Ramsay
6. Pressure-Jump Relaxation Kinetics of Unfolding and Refolding Transitions of Staphylococcal Nuclease and Proline Isomerization Mutants, Gediminas J. A. Vidugiris, Raj Thomas, and Catherine A. Royer
7. High-Pressure FTIR Studies of the Secondary Structure of Proteins, Yoshihiro Taniguchi and Naohiro Takeda
8. High-Pressure NMR Studies of the Dissociation of Arc Repressor and the Cold Denaturation of Ribonulease A, Xiangdong Peng et al.
9. Exploring Structural, Functional, and Kinetic Aspects of Nucleic Acid-Protein Complexes with Pressure: Nucleosomes and RNA Polymerase, Mauro Villas-Boas et al.
10. Pressure and Cold Denaturation of Proteins, Protein-DNA Complexes, and Viruses, Jerson L. Silva, Andrea T. Da Poian, and Debora Foguel
11. Sequence, Salt, Charge and the Stability of DNA at High Pressure, Robert B. Macgregor Jr., John Q. Wu, and Reza Najaf-Zadeh
12. Application of Pressure Relaxation to the Study of Substrate Binding to Cytochrome P-450[C[A[M versus Temperature, Pressure, and Viscosity, Gaston Hui Bon Hoa et al.
13. Pressure Effects on the Ligand-Binding Kinetics for Hemo proteins and Their Site-Directed Mutants, Isao Morishima
14. Transient Enzyme Kinetics at High Pressure, Claude Balny
15. Steady-State Enzyme Kinetics at High Pressure, Dexter Northrup
16. Effects of High Pressure on the Allosteric Properties of Phosphofructokinase from Escherichia coli, Jason L. Johnson and Gregory D. Reinhart
17. Correlation Field Splitting of Chain Vibrations: Structure and Dynamics in Lipid Bilayers and Biomembranes, Patrick T. T. Wong
18. High-Pressure Effects on the Structure of Phase Behavior of Model Membrane Systems, Roland Winter et al.
19. Membrane-Free Volume Variation with Bulky Lipid Concentration by Regular Distribution: A Functionally Important Membrane Property Explored by Pressure Studies of Phosphatidylcholine Bilayers, Parkson Lee-Gau Chong
20. NMR Studies of the Order and Dynamics of Dipalmitoylphosphatidylcholine Bilayers as a Function of Pressure, Ana Jonas et al.
21. Effects of Increased Viscosity on the Function of Integral Membrane Proteins, Suzanne F. Scarlata
22. Pressure- and Temperature-Induced Inactivation of Microorganisms, Horst Ludwig, Wilhelm Scigalla, and Bernd Sojka
23. Osmotic and Hydrostatic Pressure as Tools to Study Molecular Recognition, Stephen G. Sligar, Clifford R. Robinson, and Mark A. McLean