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AIDS is undeniably one of the most pressing medical emergencies of our era. It is now 12 years since the discovery of HIV and in spite of a world-wide research effort no proven method for controlling AIDS has been developed. HIV Volumes 1 and 2 are guides to studying HIV in the laboratory focusing on specialized techniques unique to this field. Both books provide the novice entering HIV research with clear instructions on how to perform standard experiments as well as including many novel methods described in detail for the first time.
Volume 1 describes studies of the virus and infected cells and covers techniques for handling HIV safely, for studying viral entry, replication and neutralization and basic immunological techniques.
The book contains black-and-white illustrations.
Section 1: Growth and isolation of HIV
1. Biological safety when working with HIV
2. Virological and molecular genetic techniques for studies of established HIV isolates
3. Isolation and biological characterization of primary HIV isolates
4. HIV infection of macrophages
5. HIV infection of dendritic cells
6. Vectors based on HIV
Section 2: Analysis of HIV infection
7. Quantitative assays for virus neutralization
8. Detection of HIV entry into cells
9. Detection of viral DNA by PCR
10. Detection of viral RNA by PCR
11. Sequence analysis of virus variability based on PCR
Section 3: Immunology
12. Humoral responses and detection during HIV infection
13. Quantification of CD4-positive T lymphocytes
14. CD-4 positive T-cell responses in HIV-1 infection
15. Lymphokine responses to HIV infection
16. HIV-1 specific cytotoxic T-lymphocyte responses and ADCC