HPLC of Peptides and Proteins: Methods and Protocols / Edition 1

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Overview

Hands-on experts from academia and industry comprehensively describe how to successfully perform all the critical HPLC techniques needed for the analysis of peptides and proteins. The methods range from commonly used techniques to those for capillary to large-scale preparative isolation. The authors have also presented a number of specific applications as case studies to illustrate the analytical approaches to a particular separation or assay challenge, with examples drawn from contemporary fields in biochemistry and biotechnology.

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Editorial Reviews

From the Publisher
"...a showcase of state-of-the-art methodologies in the application of HPLC to protein/peptide studies...can be used by beginners and intermediate experts in this field to develop separation and analytical prools...all scientists dealing with peptides and proteins can benefit from reading this book." - Proteomics

"...this book is well written...a very usable laboratory companion or reference guide for researchers in pursuit of peptide and protein characterizations." - Biomedical Chromatography

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Product Details

  • ISBN-13: 9780896039773
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 6/4/2008
  • Series: Methods in Molecular Biology Series , #251
  • Edition description: 2004
  • Edition number: 1
  • Pages: 414
  • Product dimensions: 9.21 (w) x 6.14 (h) x 0.94 (d)

Table of Contents

Part I. Modes of HPLC

HPLC of Peptides and Proteins: Basic Theory and Methodology

Marie-Isabel Aguilar

Reversed-Phase High-Performance Liquid Chromatography

Marie-Isabel Aguilar

Ion-Exchange Chromatography

Peter Stanton

High-Performance Hydrophobic Interaction Chromatography

Kálmán Benedek

Gel Filtration Chromatography

Peter Stanton

Hydrophilic Interaction Chromatography

Herbert Lindner and Wilfried Helliger

Immobilized Metal Ion Affinity Chromatography of Proteins

Michael Zachariou

Immunoaffinity Chromatography of Proteins

Stuart R. Gallant

Liquid Chromatography-Mass Spectrometry and Tandem Mass Spectrometry of Peptides and Proteins

Tun-Li Shen and Kathleen R. Noon

Part II. Preparative Methodologies

Capillary Separations

Montserrat Carrascal and Joaquin Abian

Micropreparative HPLC of Peptides and Proteins

Edouard C. Nice and Marie-Isabel Aguilar

Multidimensional HPLC Purification of Proteins

Edouard C. Nice and Marie-Isabel Aguilar

Analytical High-Performance Liquid Chromatography

Kálmán Benedek

Prep/Semiprep Separations of Peptides

Denis B. Scanlon and James Finlayson

Large-Scale Protein Chromatography

Joseph Bertolini, Peter Gomme, and Patrick Thomas

Part III. Applications

Proteolytic Peptide Mapping, Peter Højrup

Mass Spectrometric Characterization of Posttranslationally Modified Proteins-Phosphorylation

Martin R. Larsen

Analyses of Glycopeptides and Glycoproteins by Liquid Chromatography-Mass Spectrometry and Liquid Chromatography-Tandem Mass Spectrometry

Nana Kawasaki, Miyako Ohta, Satsuki Itoh, and Takao Hayakawa

HPLC in the Analysis of Peptide Metabolism

Rebecca A. Lew

Isolation and Characterization of Naturally Processed MHC-Bound Peptides From the Surface of Antigen-Presenting Cells

Anthony W. Purcell

Australian Funnel-Web Spider Venom Analyzed With On-Line RP-HPLC Techniques

David Wilson and Paul Alewood

HPLC and Mass Spectrometry of Intrinsic Membrane Proteins

Julian P. Whitelegge

IgG Purification

Maree S. Powell and Bruce D. Wines

DNA-Binding Proteins: LC-MS to Identify Key Domains in RNA Polymerase-Promoter Interactions

Malcolm Buckle

Sensitive Enzymatic Analysis of Histidine Decarboxylase Using HPLC

Jordi Gómez-Ramirez, Isaac Blanco, and Jordi Ortiz

Automated vs Manual Profiling of Peptide Libraries by Mass Spectrometry

Jean-Louis Aubagnac, Christine Enjalbal, Jean Martinez, Pierre Sanchez, and Gilles Subra

Proteome Analysis

Matthew J. Powell and Aaron T. Timperman

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