HPLC of Peptides and Proteins: Methods and Protocols / Edition 1

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Hands-on experts from academia and industry comprehensively describe how to successfully perform all the critical HPLC techniques needed for the analysis of peptides and proteins. The methods range from commonly used techniques to those for capillary to large-scale preparative isolation. The authors have also presented a number of specific applications as case studies to illustrate the analytical approaches to a particular separation or assay challenge, with examples drawn from contemporary fields in biochemistry and biotechnology.

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Editorial Reviews

From the Publisher
"...a showcase of state-of-the-art methodologies in the application of HPLC to protein/peptide studies...can be used by beginners and intermediate experts in this field to develop separation and analytical prools...all scientists dealing with peptides and proteins can benefit from reading this book." - Proteomics

"...this book is well written...a very usable laboratory companion or reference guide for researchers in pursuit of peptide and protein characterizations." - Biomedical Chromatography

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Product Details

  • ISBN-13: 9781617372865
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/10/2010
  • Series: Methods in Molecular Biology Series , #251
  • Edition description: Softcover reprint of hardcover 1st ed. 2004
  • Edition number: 1
  • Pages: 414
  • Product dimensions: 5.90 (w) x 9.00 (h) x 1.00 (d)

Table of Contents

Part I. Modes of HPLC

HPLC of Peptides and Proteins: Basic Theory and Methodology
Marie-Isabel Aguilar

Reversed-Phase High-Performance Liquid Chromatography
Marie-Isabel Aguilar

Ion-Exchange Chromatography
Peter Stanton

High-Performance Hydrophobic Interaction Chromatography
Kálmán Benedek

Gel Filtration Chromatography
Peter Stanton

Hydrophilic Interaction Chromatography
Herbert Lindner and Wilfried Helliger

Immobilized Metal Ion Affinity Chromatography of Proteins
Michael Zachariou

Immunoaffinity Chromatography of Proteins
Stuart R. Gallant

Liquid Chromatography-Mass Spectrometry and Tandem Mass Spectrometry of Peptides and Proteins
Tun-Li Shen and Kathleen R. Noon

Part II. Preparative Methodologies

Capillary Separations
Montserrat Carrascal and Joaquin Abian

Micropreparative HPLC of Peptides and Proteins
Edouard C. Nice and Marie-Isabel Aguilar

Multidimensional HPLC Purification of Proteins
Edouard C. Nice and Marie-Isabel Aguilar

Analytical High-Performance Liquid Chromatography
Kálmán Benedek

Prep/Semiprep Separations of Peptides
Denis B. Scanlon and James Finlayson

Large-Scale Protein Chromatography
Joseph Bertolini, Peter Gomme, and Patrick Thomas

Part III. Applications

Proteolytic Peptide Mapping, Peter Højrup

Mass Spectrometric Characterization of Posttranslationally Modified Proteins-Phosphorylation
Martin R. Larsen

Analyses of Glycopeptides and Glycoproteins by Liquid Chromatography-Mass Spectrometry and Liquid Chromatography-Tandem Mass Spectrometry
Nana Kawasaki, Miyako Ohta, Satsuki Itoh, and Takao Hayakawa

HPLC in the Analysis of Peptide Metabolism
Rebecca A. Lew

Isolation and Characterization of Naturally ProcessedMHC-Bound Peptides From the Surface of Antigen-Presenting Cells
Anthony W. Purcell

Australian Funnel-Web Spider Venom Analyzed With On-Line RP-HPLC Techniques
David Wilson and Paul Alewood

HPLC and Mass Spectrometry of Intrinsic Membrane Proteins
Julian P. Whitelegge

IgG Purification
Maree S. Powell and Bruce D. Wines

DNA-Binding Proteins: LC-MS to Identify Key Domains in RNA Polymerase-Promoter Interactions
Malcolm Buckle

Sensitive Enzymatic Analysis of Histidine Decarboxylase Using HPLC
Jordi Gómez-Ramirez, Isaac Blanco, and Jordi Ortiz

Automated vs Manual Profiling of Peptide Libraries by Mass Spectrometry
Jean-Louis Aubagnac, Christine Enjalbal, Jean Martinez, Pierre Sanchez, and Gilles Subra

Proteome Analysis
Matthew J. Powell and Aaron T. Timperman

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