Hypertension: Methods and Protocols / Edition 1

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A collection of new and essential molecular techniques for cardiovascular research. These readily reproducible methods range widely from producing congenic, consomic, transgenic, and knockout models of hypertension to the gene transfer of specific genetic material using nonviral (polymers, liposomes, and antisense agents) and adenoviral vectors. Additional techniques described include single nucleotide polymorphism (SNP) genotyping, RNA interference, microarray analysis, pharmacogenetics, and pharmacogenomics for the genetic dissection of hypertension, as well as a practical method for deriving cardiomyocytes from embryonic stem cells that would serve as replacement cells for those damaged by hypertension or heart attack. The book offers both novice and experienced hypertension researchers an indispensable collection of readily reproducible techniques for successful research, work that has already dramatically improved the outlook for hypertensive patients, and promises much future success.
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Editorial Reviews

Doody's Review Service
Reviewer: Michael C Cunningham, M.D.(Ochsner Clinic Foundation)
Description: This multiauthored book on hypertension is divided into seven distinct sections. The first section demonstrates models of hypertension with each subsequent section focusing on particular research modalities and protocols used to investigate the pathophysiology of hypertension.
Purpose: The book is intended to educate readers about hypertension, updating their knowledge of the current methods in hypertensive research and stimulating new ideas for further investigation.
Audience: Intended for novice and expert researchers in the field, this book concentrates on the molecular side of research. The authors specialize in this area and have gathered a wealth of information and techniques from several other sources. With this knowledge, these experts have created the reference in this field.
Features: Initially, the book demonstrates how to engineer various genetically altered mice as models for hypertension. Then, in a well outlined fashion, it discusses and expounds on the techniques of pharmacogenetics and pharmacogenomics. This includes various protein analyses and gene transfer using viral vectors and then on to assorted aspects of stem cell research. There is also a section dedicated to information technology and its indispensable role in pharmacogenomics data collection and interpretation. The illustrations are particularly well placed, usually complementing or illustrating a confusing method.
Assessment: The authors have created a much needed book in the area of molecular hypertension that serves beginners and experts in the field, not only to help them continue their current work, but also to stimulate new thought processes and ideas that will provide further insight into this deadly disease and identify new targets for pharmacologic therapy.

3 Stars from Doody
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Product Details

  • ISBN-13: 9781588293237
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 12/27/2004
  • Series: Methods in Molecular Medicine Series, #108
  • Edition description: 2005
  • Edition number: 1
  • Pages: 502
  • Product dimensions: 9.21 (w) x 6.14 (h) x 1.13 (d)

Table of Contents

Part I. Models of Hypertension

Congenic/Consomic Models of Hypertension Delyth Graham, Martin W. McBride, Nicholas J. R. Brain, and Anna F. Dominiczak

Mouse Knockout Models of Hypertension Michael Bader

Production of Transgenic Models in Hypertension Elena Popova, Michael Bader, and Alexander Krivokharchenko

Measuring Blood Pressure in Small Laboratory Animals Klaas Kramer and René Remie

Part II. Assessment of Free Radicals in Endothelial Dysfunction

Analysis of Superoxide Anion Production in Tissue Michela Zanetti, Livius V. d'Uscio, Timothy E. Peterson, Zvonimir S. Katusic, and Timothy O'Brien

Measurement of Vascular Reactive Oxygen Species Production by Chemiluminescence Tomasz J. Guzik and Keith M. Channon

A Guide to Wire Myography Angela Spiers and Neal Padmanabhan

Part III. Nucleic Acid Techniques

Selection of Candidate Genes in Hypertension Charles A. Mein, Mark J. Caulfield, and Patricia B. Munroe

Genome-Wide Scanning With SSLPs in the Rat Carol Moreno, Kathleen Kennedy, Jaime Wendt Andrae, and Howard J. Jacob

Extraction of RNA From Cells and Tissue Ian M. Bird

Single-Strand Conformational Polymorphism Analysis: Basic Principles and Routine Practice Yanbin Dong and Haidong Zhu

Single-Nucleotide Polymorphism Genotyping for Disease Association Studies Myriam Fornage and Peter A. Doris

Denaturing High-Performance Liquid Chromatography Using the WAVE DNA Fragment Analysis System Elizabeth Donohoe

TaqMan Real-Time PCR Quantification: Conventional and Modified Methods Michihiro Yoshimura, Shota Nakamura, and Hisao Ogawa

Generation of High-Sensitivity Antisense cDNA Probes by Asymmetric PCR Ian M. Bird

New Advances in Microarrays: Finding the Genes Causally Involved in Disease Claudio Napoli, Filomena de Nigris, and Vincenzo Sica

Pharmacogenetics and Pharmacogenomics Klaus Lindpaintner

Part IV. Proteins and Proteomics

Determination of the Endogenous Nitric Oxide Synthase Inhibitor Asymmetric Dimethylarginine in Biological Samples by HPLC Tom Teerlink

Proteomic Approaches in the Analysis of Hypertension Soren Naaby-Hansen, Gayathri D. Warnasuriya, Claire Hastie, Piers Gallney, and Rainer Cramer

Part V. Gene Transfer

Gene Transfer in Endothelial Dysfunction and Hypertension Sabyasachi Sen, Padraig M. Strappe, and Timothy O'Brien

Nonviral Gene Delivery Methods in Cardiovascular Diseases Marika Ruponen, Zanna Hyvönen, Arto Urtti, and Seppo Ylä-Herttuala

Construction and Characterization of Helper-Dependent Adenoviral Vectors for Sustained In Vivo Gene Therapy Kazuhiro Oka and Lawrence Chan

Adenovirus-Mediated Gene Transfer In Vivo: An Approach to Reduce Oxidative Stress Yi Chu

Gene Therapy for Hypertension: Antisense Inhibition of the Renin-Angiotensin System M. Ian Phillips and Birgitta Kimura

Modulation of Gene Expression by RNAi Cezary Wójcik, Rosalind Fabunmi, and George N. DeMartino

In Vivo Biopanning: A Methodological Approach to Identifying Novel Targeting Ligands for Delivery of Biological Agents to the Vasculature Lorraine M. Work, Campbell G. Nicol, Laura Denby, and Andrew H. Baker

Part VI. Stem Cells

Cardiomyocytes Derived From Embryonic Stem Cells Kenneth R. Boheler, David G. Crider, Yelena Tarasova, and Victor A. Maltsev

Part VII. Bioinformatics

In Silico and Wet-Bench Identification of Nuclear Matrix Attachment Regions Stephen A. Krawetz, Sorin Draghici, Robert Goodrich, Zhandong Liu, and G. Charles Ostermeier

Biomedical Informatics Methods in Pharmacogenomics Qing Yan


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