Identification And Characterization Of Xylose Transporters, Xut1 And Sut4, Of Pichia Stipitis.

Overview

Yeast strain Saccharomyces cerevisiae has become important host for heterologous gene expression. The control of gene expression, therefore, is important for optimal metabolic engineering. To improve transcriptional control in heterologous expression, the effects of gene orientation on growth, transcription, and enzyme activity were analyzed and there was no significant effect of gene orientation. The possible dilution of promoter strength due to multiple use of the same promoter was also tested and no ...
See more details below
This Paperback is Not Available through BN.com
Sending request ...

More About This Book

Overview

Yeast strain Saccharomyces cerevisiae has become important host for heterologous gene expression. The control of gene expression, therefore, is important for optimal metabolic engineering. To improve transcriptional control in heterologous expression, the effects of gene orientation on growth, transcription, and enzyme activity were analyzed and there was no significant effect of gene orientation. The possible dilution of promoter strength due to multiple use of the same promoter was also tested and no significant effect was found. According to our data, there is no significant effect of gene orientation or multiple promoter use on gene transcription and translation when genes are expressed from plasmids, however, other factors could affect expression of adjacent genes in chromosomes. Pichia slipitis is one of the most efficient xylose-utilizing yeasts and xylose transporters of this strain have been identified. Xut1 has a higher affinity for xylose (Km = 0.46 +/- 0.02 mM) than glucose (Km = 0.91 +/- 0.01 mM), and a higher Vmax for xylose than glucose (116.3 +/- 5.8 vs 80.0 +/- 0.8 mmol/min/mg). Xut1 has been identified as a symporter. The other sugar transporter Sut4 also showed xylose-transporting activity, however, it has a more than 10-fold higher affinity for glucose (Km = 1.3 +/- 0.1 mM) than xylose (Km = 16.6 +/- 0.3 mM). Although some reduction in sugar transport activity was observed after sodium azide treatment, Sut4 has been characterized as a facilitator. The expression of these two genes in a hexose transport deficient strain of S. cerevisiae engineered for xylose metabolism restored growth on both glucose and xylose. Expression of these genes in another lab strain of S. cerevisiae enhanced growth on xylose and increased ethanol production from glucose. These findings demonstrated that XUT1 and SUT4 affect sugar influx and fermentation. As sugar uptake is an important factor in achieving efficient sugar utilization, these findings represent a significant development in the production of more efficient bioethanol producing yeasts.
Read More Show Less

Product Details

  • ISBN-13: 9781243599124
  • Publisher: BiblioLabsII
  • Publication date: 9/4/2011
  • Pages: 114
  • Product dimensions: 7.44 (w) x 9.69 (h) x 0.24 (d)

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star

(0)

4 Star

(0)

3 Star

(0)

2 Star

(0)

1 Star

(0)

Your Rating:

Your Name: Create a Pen Name or

Barnes & Noble.com Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & Noble.com that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & Noble.com does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at BN.com or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation

Reminder:

  • - By submitting a review, you grant to Barnes & Noble.com and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Noble.com Terms of Use.
  • - Barnes & Noble.com reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & Noble.com also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on BN.com. It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

 
Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)