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Doody's Review ServiceReviewer: Eugene A Davidson, PhD (Georgetown University School of Medicine)
Description: This is a guide to the identification and expression of G protein-coupled receptors.
Purpose: This volume is intended as a "how-to" laboratory accessory for investigators wanting to express G protein-coupled receptors.
Audience: Investigators with a need to express such receptors are the intended audience, but they will have difficulty in decision making since there is a lack of critical commentary on the pros and cons of the various technologies.
Features: The coverage is reasonably complete in that the most common methods of protein expression are described — bacteria, mammalian cells, insect cells, oocytes, and yeast. Attention is also given to the growing problem of information management and there is an extensive listing of cloned receptors together with accession data.
Assessment: It is not uncommon for a given protein to be effectively expressed by one method (e.g., yeast) and fail miserably in a different genetic background. Given a new candidate, how does an investigator decide on the best strategic approach? If one is willing to try several, this volume will have value. If resources are limited so that the shotgun approach is not feasible, prudent consultation is likely to provide a better outcome. It also should be noted that several of the chapters provide descriptive material as opposed to protocols. In this regard, the utility of the book as a laboratory adjunct is limited.