Imaging of Nucleic Acids and Quantitation in Photonic Microscopy

by Xavier Ronot (Editor), Yves Usson (Editor)

Overview

Visualization of nuclear components has added tremendously to our understanding of the nucleus and its chemistry. The fluorescence approach is considered the present-day standard for comprehending nuclear organization. Imaging of Nucleic Acids and Quantitation in Photonic Microscopy provides new technologies in fluorescence microscopy and image cyt

Product Details

ISBN-13:	9781040202302
Publisher:	CRC Press
Publication date:	05/30/2001
Series:	Methods in Visualization
Sold by:	Barnes & Noble
Format:	eBook
Pages:	184
File size:	5 MB

Table of Contents

Fluorochromes and Fluorescent Probes. Nucleic Probes. Comet Assay. High Resolution FISH Mapping. Fluorescence Imaging. 3D Visualization and Image Processing. Fluorescence Image Analysis. Example of Quantitation.

Editorial Reviews

Serves as a tutorial for researchers interested in the analysis of nucleic acids by image cytometry following fluorescent labeling and detection procedures. Chapters are written by researchers with extensive experience in the use of photonic microscopy in the field of nucleic acid research. Two chapters examine the design of probes that enable direct or indirect labeling of nucleic acids. Other chapters describe methodologies developed for microscopic analysis of nucleic acids, and outline fluorescent imaging principles for the study of nuclear distribution and colocalization of molecules. A final chapter gives examples of quantification in chromatin organization in cells maintained alive, and distribution of nucleolar bodies inside the nucleus. Includes b&w images. Ronot teaches at the Ecole Pratique des Hautes Etudes. Usson is a researcher at the Centre National de la Recherche Scientifique. Annotation c. Book News, Inc., Portland, OR (booknews.com)

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