Immobilization of Enzymes and Cells / Edition 2

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For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.

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Editorial Reviews

From the Publisher
"Many well-known authors from research laboratories worldwide have contributed to this successful volume. . .this book provides a basic reference tool for all those who will need to immobilize biological materials in their work-not only for the beginner in the field, but also for experienced researchers."-Applied Biochemistry and Biotechnology

From Reviews of the First Edition...

"...enables researchers to rapidly enter the world of excellent reference work that will have long lasting value." -Quarterly Review of Biology

" of the most comprehensive collections of immobilization methodologies...[will] find extensive use as a methodology/reference text in any biotechnology-based laboratory." -Microbiology Today

"...a valuable tool for researchers in the many areas requiring enzyme and cell immobilizations." - Trends in Biotechnology

" ideal reference book to have around the laboratory." -Trends in Cell Biology

"...a worthwhile addition to any research library...especially useful for anyone involved in preparative isolations or needing immobilized reagents for specific research applications." -Doody's Health Sciences Book Review Journal

"...the coverage of the various techniques is excellent..." -The Analyst

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Product Details

  • ISBN-13: 9781588292902
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 3/15/2006
  • Series: Methods in Biotechnology Series, #22
  • Edition description: 2nd ed. 2006
  • Edition number: 2
  • Pages: 450
  • Product dimensions: 9.21 (w) x 6.14 (h) x 1.00 (d)

Table of Contents

1. New Opportunities for Immobilization of Enzymes

Jose M. Guisan

2. Immobilization of Enzymes: A Literature SurveyBeatriz Brena, Paula González-Pombo, and Francisco Batista-Viera

3. Glutaraldehyde Mediated Protein Immobilization

Fernando López-Gallego, Jose M. Guisán,and Lorena Betancor

4. Immobilization of Enzymes on Mono-and Hetero-Functional Epoxy-Activated Supports

Cesar Mateo, Valeria Grazu, and Jose M. Guisan

5. Stabilization of Enzymes by Multipoint Covalent Immobilization on Supports Activated with Glyoxyl Groups

Fernando López-Gallego, Gloria Fernandez-Lorente, Javier Rocha-Martin, Juan M. Bolivar, Cesar Mateo and Jose M. Guisan

6. Oriented Covalent Immobilization of Enzymes on Heterofunctional-Glyoxyl Supports

Cesar Mateo, Gloria Fernandez-Lorente, Rocha-Martin, J., Bolivar, Juan, M., Jose M. Guisan

7. Reversible Covalent Immobilization of Enzymes Via Disulfide Bonds

Karen Ovsejevi , Carmen Manta, and Francisco Batista-Viera

8. Immobilization of Candida rugosa Lipase on Superparamagnetic Fe3O4 Nanoparticles for Biocatalysis in Low Water Media

Joyeeta Mukherjee, Kusum Solanki, and Munishwar Nath Gupta

9. Immobilization of Enzymes by Bioaffinity Layering

Veena Singh, Meryam Sardar, and Munishwar Nath Gupta

10. Immobilization of Enzymes on Magnetic Beads through Affinity Interactions

Audrey Sassolas, Akhtar Hayat and Jean-Louis Marty

11. Tips for the Functionalization of Nanoparticles with Antibodies

Ester Polo, Sara Puertas, María Moros, Pilar Batalla, José M. Guisán, Jesús M. de la Fuente, Valeria Grazú

12. Design and Characterization of Functional Nanoparticles for Enhanced Bio-Performance

Pablo del Pino, Scott G. Mitchell & Beatriz Pelaz

13. Immobilization of Enzymes on Ethynyl-modified Electrodes Via Click Chemistry

Akhtar Hayat, Audrey Sassolas, Amina Rhouati, Jean-Louis Marty

14. Modification of Carbon Nanotube Electrodes with 1-pyrenebutanoic Acid, Succinimidyl Ester for Enhanced Bioelectrocatalysis

Guinevere Strack, Robert Nichols, Plamen Atanassov, Heather R. Luckarift, and Glenn R. Johnson

15. Enzyme Immobilization by Entrapment within a Gel Network

Audrey Sassolas, Akhtar Hayat and Jean-Louis Marty

16. Practical Protocols for Lipase Immobilization via Sol-Gel Techniques

Manfred T. Reetz

17. Improving Lipase Activity by Immobilization and Post-Immobilization Strategies

Jose M. Palomo, Marco Filice, Oscar Romero and Jose M. Guisan

18. High Activity Preparations of Lipases and Proteases for Catalysis in Low Water Containing Organic Solvents and Ionic Liquids

Ipsita Roy, Joyeeta Mukherjee, and Munishwar Nath Gupta

19. Biomedical Applications of Immobilized Enzymes: An Update

Marta Pastor, Amaia Esquisabel, and José Luis Pedraz

20. Immobilization of Whole Cells by Chemical Vapor Deposition of Silica

Susan R. Sizemore, Robert Nichols, Randi Tatum, Plamen Atanassov, Glenn R. Johnson, and Heather R. Luckarift

21. Encapsulation of Cells in Alginate Gels

Pello Sánchez, Rosa María Hernández, José Luis Pedraz, and Gorka Orive

22. Microalgal Immobilization Methods

Ignacio Moreno-Garrido

23. Therapeutic Applications of Encapsulated Cells

Argia Acarregui, Gorka Orive, José Luis Pedraz, and Rosa María Hernández

24. Whole Cell Entrapment Techniques

Jorge A. Trelles and Cintia W. Rivero

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