Immobilization of Enzymes and Cells / Edition 2

Immobilization of Enzymes and Cells / Edition 2

by Jose M. Guisan
     
 

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ISBN-10: 1617374733

ISBN-13: 9781617374739

Pub. Date: 11/19/2010

Publisher: Springer-Verlag New York, LLC

The continuing rapid progress in work designed to improve the functional properties of enzymes and cells as industrial catalysts has led to this revised, updated, and expanded new edition of the warmly received initial edition of Immobilization of Enzymes and Cells. This long-awaited second edition contains new and simplified prools useful for industrial

Overview

The continuing rapid progress in work designed to improve the functional properties of enzymes and cells as industrial catalysts has led to this revised, updated, and expanded new edition of the warmly received initial edition of Immobilization of Enzymes and Cells. This long-awaited second edition contains new and simplified prools useful for industrial applications, novel techniques that will prove useful now or in the near future, and prools for the preparation of immobilized derivatives suitable for a wide variety of nonconventional reaction media. The authors also offer tools for the development of new immobilization techniques, methods for preparing immobilized derivatives for therapeutic and industrial use, and new chemical reactors designed to overcome the limitations of immobilized derivatives. The emphasis is on improving enzyme and cell properties via very simple immobilization prools, along with the development of new and better methods. The prools follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Innovative and highly practical, Immobilization of Enzymes and Cells, Second Edition, affords biochemists, biotechnologists, and biochemical engineers a practical review of all the latest methods and tools-as well as optimized conventional techniques-needed to carry out successful research involving immobilizing enzymes and cells.

Product Details

ISBN-13:
9781617374739
Publisher:
Springer-Verlag New York, LLC
Publication date:
11/19/2010
Series:
Methods in Biotechnology Series, #22
Edition description:
Softcover reprint of hardcover 2nd ed. 2006
Pages:
450
Product dimensions:
5.98(w) x 9.02(h) x 0.04(d)

Table of Contents

Immobilization of Enzymes as the 21st Century Begins: An Already Solved Problem or Still an Exciting Challenge?

Jose M. Guisan

Immobilization of Enzymes: A Literature Survey

Beatriz M. Brena and Franciso Batista-Viera

Cross-Linked Enzyme Aggregates

Roger A. Sheldon, Rob Schoevaart, and Luuk M. van Langen

Immobilization-Stabilization of Enzymes by Multipoint Covalent Attachment on Supports Activated With Epoxy Groups

Cesar Mateo, Olga Abian, Gloria Fernández-Lorente, Benevides C. C. Pessela, Valeria Grazu, Jose M. Guisan, and Roberto Fernandez-Lafuente

Glutaraldehyde in Protein Immobilization: A Versatile Reagent

Lorena Betancor, Fernando López-Gallego, Noelia Alonso-Morales, Gisella Dellamora, Cesar Mateo, Roberto Fernandez-Lafuente, and Jose M. Guisan

Practical Prools for Lipase Immobilization Via Sol-Gel Techniques

Manfred T. Reetz

Encapsulation of Enzymes Using Polymers and Sol-Gel Techniques

Mònica Campàs and Jean-Louis Marty

Design of Smart Biocatalysts: Immobilization of Enzymes on Smart Polymers

Ipsita Roy and Munishwar N. Gupta

Affinity Immobilization of Tagged Enzymes

Silvana Andreescu, Bogdan Bucur, and Jean-Louis Marty

Bioaffinity Immobilization

Ipsita Roy and Munishwar N. Gupta

One-Step Purification, Immobilization, and Stabilization of Poly-Histidine-Tagged Enzymes Using Metal Chelate-Epoxy Supports

Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Rodrigo Torres, Fernando López-Gallego, Jose M. Guisan, and Roberto Fernandez-Lafuente

Stabilization of Multimeric Enzymes Via Immobilization and Further Cross-Linking With Aldehyde-Dextran

Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Lorena Betancor, Aurelio Hidalgo, Gloria Fernández-Lorente, Roberto Fernandez-Lafuente, and Jose M. Guisan

Purification, Immobilization, Hyperactivation, and Stabilization of Lipases by Selective Adsorption on Hydrophobic Supports

Jose M. Palomo, Gloria Fernández-Lorente, Cesar Mateo, Rosa L. Segura, Claudia Ortiz, Roberto Fernandez-Lafuente, and Jose M. Guisan

Immobilization and Stabilization of Proteins by Multipoint Covalent Attachment on Novel Amino-Epoxy-Sepabeads®

Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Fernando López-Gallego, Rodrigo Torres, Manuel Fuentes, Aurelio Hidalgo, Jose M. Palomo, Lorena Betancor, Gloria Fernández-Lorente, Claudia Ortiz, Olga Abian, Jose M. Guisan, and Roberto Fernandez-Lafuente

Improved Stabilization of Chemically Aminated Enzymes Via Multipoint Covalent Attachment on Glyoxyl Supports

Tamara Montes, Fernando López-Gallego, Manuel Fuentes, Cesar Mateo, Valeria Grazu, Lorena Betancor, Jose M. Guisan, and Roberto Fernandez-Lafuente

Stabilization of New Imprint Property of Glucose Oxidase in Pure Aqueous Medium by Cross-Linked-Imprinting Approach

Alankar Vaidya and Lutz Fischer

Reversible Covalent Immobilization of Enzymes Via Their Thiol Groups

Francisco Batista-Viera, Karen Ovsejevi, and Carmen Manta

Very Strong But Reversible Immobilization of Enzymes on Supports Coated With Ionic Polymers

Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Claudia Ortiz, Fernando López-Gallego, Lorena Betancor, Noelia Alonso-Morales, Jose M. Guisan, and Roberto Fernandez-Lafuente

Immobilization of Enzymes on Magnetic Particles

Martina Koneracká, Peter Kopcans&kacute;y , Milan Timko, Chenyl Nynitapal Ramchand, Zainul M. Saiyed, Michael Trevan, and Anil de Sequeira

Immobilization of Proteins on Gold Surfaces

José M. Abad, Marcos Pita, and Víctor M. Fernández

Immobilization of Enzymes on Electrodes

Gilvanda Silva Nunes and Jean-Louis Marty

Immobilization of Enzymes for Use in Organic Media

Patrick Adlercreutz

Immobilization of Enzymes for Use in Ionic Liquids

Pedro Lozano, Teresa de Diego, and José L. Iborra

Immobilization of Enzymes for Use in Supercritical Fluids

Pedro Lozano, Teresa de Diego, and José L. Iborra

Immobilized Enzymes for Biomedical Applications

Amaia Esquisabel, Rosa María Hernández, Alicia Rodríguez Gascón, and José Luis Pedraz

Characterization of Immobilized Enzymes by Microcalorimetry

Ezio Battistel and Giovanni Rialdi

Use of Immobilized Biocatalysts in Fluidized Bed Format

Ipsita Roy and Munishwar N. Gupta

Taylor-Couette Vortex Flow in Enzymatic Reactors

Roberto Campos Giordano and Raquel de Lima Camargo Giordano

A Novel Immobilization Method for Entrapment: LentiKats®

Marc Schlieker and Klaus-Dieter Vorlop

Encapsulation of Cells in Alginate Gels

Gorka Orive, Rosa María Hernández, Alicia Rodríguez Gascón, and José Luis Pedraz

Immobilization of Cells on Polyurethane Foam

Ignacio de Ory, Gema Cabrera, Martin Ramirez, and Ana Blandino

Immobilization of Cells With Transition Metal

Pedro Fernandes

Immobilization of Microalgae

Nirupama Mallick

Bioluminescence in Immobilized Cells for Biomass Detection and Biosensor Applications

Marián Navrátil, Juraj Svitel and Peter Gemeiner

A Proteomic Approach to Biofilm Cell Physiology

Laurent Coquet, Sébastien Vilain, Pascal Cosette, Thierry Jouenne, and Guy-Alain Junter

Encapsulation of Bacteria for Biodegradation of Gasoline Hydrocarbons

Peyman Moslemy, Serge R. Guiot, and Ronald J. Neufeld

Biomedical Applications of Immobilized Cells

Gorka Orive, Rosa María Hernández, Alicia Rodríguez Gascón, and José Luis Pedraz

Index

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