Immobilization of Enzymes and Cells / Edition 2

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For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.

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Editorial Reviews

From the Publisher
"Many well-known authors from research laboratories worldwide have contributed to this successful volume. . .this book provides a basic reference tool for all those who will need to immobilize biological materials in their work-not only for the beginner in the field, but also for experienced researchers."-Applied Biochemistry and Biotechnology

From Reviews of the First Edition...

"...enables researchers to rapidly enter the world of excellent reference work that will have long lasting value." -Quarterly Review of Biology

" of the most comprehensive collections of immobilization methodologies...[will] find extensive use as a methodology/reference text in any biotechnology-based laboratory." -Microbiology Today

"...a valuable tool for researchers in the many areas requiring enzyme and cell immobilizations." - Trends in Biotechnology

" ideal reference book to have around the laboratory." -Trends in Cell Biology

"...a worthwhile addition to any research library...especially useful for anyone involved in preparative isolations or needing immobilized reagents for specific research applications." -Doody's Health Sciences Book Review Journal

"...the coverage of the various techniques is excellent..." -The Analyst

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Product Details

  • ISBN-13: 9781617374739
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 11/19/2010
  • Series: Methods in Biotechnology Series , #22
  • Edition description: Softcover reprint of hardcover 2nd ed. 2006
  • Edition number: 2
  • Pages: 450
  • Product dimensions: 6.00 (w) x 9.00 (h) x 1.03 (d)

Table of Contents

Immobilization of Enzymes as the 21st Century Begins: An Already Solved Problem or Still an Exciting Challenge?

Jose M. Guisan

Immobilization of Enzymes: A Literature Survey

Beatriz M. Brena and Franciso Batista-Viera

Cross-Linked Enzyme Aggregates

Roger A. Sheldon, Rob Schoevaart, and Luuk M. van Langen

Immobilization-Stabilization of Enzymes by Multipoint Covalent Attachment on Supports Activated With Epoxy Groups

Cesar Mateo, Olga Abian, Gloria Fernández-Lorente, Benevides C. C. Pessela, Valeria Grazu, Jose M. Guisan, and Roberto Fernandez-Lafuente

Glutaraldehyde in Protein Immobilization: A Versatile Reagent

Lorena Betancor, Fernando López-Gallego, Noelia Alonso-Morales, Gisella Dellamora, Cesar Mateo, Roberto Fernandez-Lafuente, and Jose M. Guisan

Practical Prools for Lipase Immobilization Via Sol-Gel Techniques

Manfred T. Reetz

Encapsulation of Enzymes Using Polymers and Sol-Gel Techniques

Mònica Campàs and Jean-Louis Marty

Design of Smart Biocatalysts: Immobilization of Enzymes on Smart Polymers

Ipsita Roy and Munishwar N. Gupta

Affinity Immobilization of Tagged Enzymes

Silvana Andreescu, Bogdan Bucur, and Jean-Louis Marty

Bioaffinity Immobilization

Ipsita Roy and Munishwar N. Gupta

One-Step Purification, Immobilization, and Stabilization of Poly-Histidine-Tagged Enzymes Using Metal Chelate-Epoxy Supports

Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Rodrigo Torres, Fernando López-Gallego, Jose M. Guisan, and Roberto Fernandez-Lafuente

Stabilization of Multimeric Enzymes Via Immobilization and Further Cross-Linking With Aldehyde-Dextran

Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Lorena Betancor, Aurelio Hidalgo, Gloria Fernández-Lorente, Roberto Fernandez-Lafuente, and Jose M. Guisan

Purification, Immobilization, Hyperactivation, and Stabilization of Lipases by Selective Adsorption on Hydrophobic Supports

Jose M. Palomo, Gloria Fernández-Lorente, Cesar Mateo, Rosa L. Segura, Claudia Ortiz, Roberto Fernandez-Lafuente, and Jose M. Guisan

Immobilization and Stabilization of Proteins by Multipoint Covalent Attachment on Novel Amino-Epoxy-Sepabeads®

Cesar Mateo, Benevides C. C. Pessela, Valeria Grazu, Fernando López-Gallego, Rodrigo Torres, Manuel Fuentes, Aurelio Hidalgo, Jose M. Palomo, Lorena Betancor, Gloria Fernández-Lorente, Claudia Ortiz, Olga Abian, Jose M. Guisan, and Roberto Fernandez-Lafuente

Improved Stabilization of Chemically Aminated Enzymes Via Multipoint Covalent Attachment on Glyoxyl Supports

Tamara Montes, Fernando López-Gallego, Manuel Fuentes, Cesar Mateo, Valeria Grazu, Lorena Betancor, Jose M. Guisan, and Roberto Fernandez-Lafuente

Stabilization of New Imprint Property of Glucose Oxidase in Pure Aqueous Medium by Cross-Linked-Imprinting Approach

Alankar Vaidya and Lutz Fischer

Reversible Covalent Immobilization of Enzymes Via Their Thiol Groups

Francisco Batista-Viera, Karen Ovsejevi, and Carmen Manta

Very Strong But Reversible Immobilization of Enzymes on Supports Coated With Ionic Polymers

Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Claudia Ortiz, Fernando López-Gallego, Lorena Betancor, Noelia Alonso-Morales, Jose M. Guisan, and Roberto Fernandez-Lafuente

Immobilization of Enzymes on Magnetic Particles

Martina Koneracká, Peter Kopcans&kacute;y , Milan Timko, Chenyl Nynitapal Ramchand, Zainul M. Saiyed, Michael Trevan, and Anil de Sequeira

Immobilization of Proteins on Gold Surfaces

José M. Abad, Marcos Pita, and Víctor M. Fernández

Immobilization of Enzymes on Electrodes

Gilvanda Silva Nunes and Jean-Louis Marty

Immobilization of Enzymes for Use in Organic Media

Patrick Adlercreutz

Immobilization of Enzymes for Use in Ionic Liquids

Pedro Lozano, Teresa de Diego, and José L. Iborra

Immobilization of Enzymes for Use in Supercritical Fluids

Pedro Lozano, Teresa de Diego, and José L. Iborra

Immobilized Enzymes for Biomedical Applications

Amaia Esquisabel, Rosa María Hernández, Alicia Rodríguez Gascón, and José Luis Pedraz

Characterization of Immobilized Enzymes by Microcalorimetry

Ezio Battistel and Giovanni Rialdi

Use of Immobilized Biocatalysts in Fluidized Bed Format

Ipsita Roy and Munishwar N. Gupta

Taylor-Couette Vortex Flow in Enzymatic Reactors

Roberto Campos Giordano and Raquel de Lima Camargo Giordano

A Novel Immobilization Method for Entrapment: LentiKats®

Marc Schlieker and Klaus-Dieter Vorlop

Encapsulation of Cells in Alginate Gels

Gorka Orive, Rosa María Hernández, Alicia Rodríguez Gascón, and José Luis Pedraz

Immobilization of Cells on Polyurethane Foam

Ignacio de Ory, Gema Cabrera, Martin Ramirez, and Ana Blandino

Immobilization of Cells With Transition Metal

Pedro Fernandes

Immobilization of Microalgae

Nirupama Mallick

Bioluminescence in Immobilized Cells for Biomass Detection and Biosensor Applications

Marián Navrátil, Juraj Svitel and Peter Gemeiner

A Proteomic Approach to Biofilm Cell Physiology

Laurent Coquet, Sébastien Vilain, Pascal Cosette, Thierry Jouenne, and Guy-Alain Junter

Encapsulation of Bacteria for Biodegradation of Gasoline Hydrocarbons

Peyman Moslemy, Serge R. Guiot, and Ronald J. Neufeld

Biomedical Applications of Immobilized Cells

Gorka Orive, Rosa María Hernández, Alicia Rodríguez Gascón, and José Luis Pedraz


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