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Immunoassays: A Practical Approach / Edition 1
     

Immunoassays: A Practical Approach / Edition 1

by James P. Gosling, James P. Gosling
 

ISBN-10: 0199637105

ISBN-13: 9780199637102

Pub. Date: 06/28/2000

Publisher: Oxford University Press, USA

Immunoassays is the first practical volume designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are described.

Overview

Immunoassays is the first practical volume designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are described. Solid-phase reagents, standards and immunogens are explained, with procedures for their preparation. But most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to combining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control give valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples.

Product Details

ISBN-13:
9780199637102
Publisher:
Oxford University Press, USA
Publication date:
06/28/2000
Series:
Practical Approach Series , #228
Edition description:
New Edition
Pages:
328
Product dimensions:
9.30(w) x 7.10(h) x 0.70(d)

Related Subjects

Table of Contents

Preface
Protocol List
Abbreviations
1. Analysis by specific binding, James P. Gosling
2. Raising antibodies, Marian M. Kane and John N. Banks
3. Choosing and characterizing antibodies, Anthony Forde and John Coley
4. Labels and endpoints, Wajdi Abdul-Ahad and Martin Brett
5. Solid-phase reagents, Robert S. Matson
6. Standards and immunogens, M. Quasim Chaudry
7. Optimization, Eric Ezan and Jacques Grassi
8. Validation, Peter O'Fegan
9. Data processing, Barry Nix and David Wild
10. Quality assurance, John Seth
A1. Suitable reagents and other requisites
A2. List of suppliers
Index

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