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Immunochemical Protocols / Edition 3

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This new edition of a much praised classic includes both basic method for producing and using antibodies and advanced techniques required by today's state-of-the art investigators. The basic sections of the book stress the first principles of antibody production and guide the reader through the processes and pitfalls of immunochemistry. The advanced sections focus on specific applications and cover the immunochemical techniques now used in laboratories. New material includes techniques for making new monoclonal cell lines, for producing good quality monoclonal antibodies, immunocapture PCR, immunoaffinity purification, and immunoelectron microscopy in plant virus diagnosis.

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Editorial Reviews

Doody's Review Service
Reviewer: Eugene A Davidson, PhD (Georgetown University School of Medicine)
Description: This book provides a set of immunochemical protocols.
Purpose: The goal is to provide a set of immunochemical protocols usable by relatively inexperienced investigators. While a generally useful objective, several drawbacks limit the utility of this contribution.
Audience: The target audience is the laboratory worker (advanced student, research fellow). The editor has recruited about 20 contributors, mainly from the U.K. The editor has limited name recognition.
Features: Most investigators have use for antibodies. While there are many commercial sources for such reagents, on occasion it is preferable to prepare these materials in the home laboratory. This book offers protocols with sufficient detail to allow implementation of many immunochemical methods. Included are techniques for monoclonal preparation, immuno-electron microscopy, conjugation procedures, purification, and the relatively new method of immuno-polymerase chain reaction. Each chapter provides protocols for the method that are sufficiently detailed to allow ready application. Appended bibliographies range from adequate to meager; illustrations are essentially absent. In some respects, the contributions seem dated. Thus, mention is made of the broad efficacy of Freund's adjuvant, generally prohibited at most U.S. institutions. Likewise, it may be of interest to have a protocol for coupling an antibody to horseradish peroxidase but virtually any investigator will purchase this material. This is also true for most antibodies. The limited scope of this book, coupled with the lack of an overview of techniques (including their limitations) dampens enthusiasm. Investigators wishing to have some basic protocols available will find useful material.
Assessment: This is not a strong effort. The author list is somewhat provincial, the advantages of various methods not adequately discussed, and the material dated. Chapters are of uneven quality — a firm editorial hand is missing.

2 Stars from Doody
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Product Details

  • ISBN-13: 9781588292742
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 12/13/2004
  • Series: Methods in Molecular Biology Series , #295
  • Edition description: 3rd ed. 2005
  • Edition number: 3
  • Pages: 318
  • Product dimensions: 9.21 (w) x 6.14 (h) x 0.75 (d)

Table of Contents

Immunization Strategies for Antibody Production
Robert Burns

Synthetic Peptides as Antigens for Antibody Production
David C. Hancock and Nicola J. O'Reilly

Production of Polyclonal Antibodies in Rabbits
David C. Hancock and Nicola J. O'Reilly

Making Hybridomas
Robert Burns

Growing Hybridomas
Gary Entrican and Gareth Young

Phage Display Vectors for the In Vitro Generation of Human Antibody Fragments
Michael Hust and Stefan Dübel

Purification of Antibodies and Preparation of Antibody Fragments
Graham D. Kemp

Conjugation of Antibodies to Alkaline Phosphatase
G. Brian Wisdom

Conjugation of Antibodies to Horseradish Peroxidase
G. Brian Wisdom

Conjugation of Antibodies to Fluorescein or Rhodamine
G. Brian Wisdom

Conjugation of Antibodies to Biotin
James D. Hirsch and Rosaria P. Haugland

Preparation of Colloidal Gold Probes
David Hughes

Immunogold Probes in Light Microscopy
David Hughes

Immunoelectron Microscope Techniques in Plant Virus Diagnosis
Daphne M. Wright

Dual-Antibody Sandwich Enzyme-Linked Immunosorbent Assay
William Jordan

Competitive Enzyme-Linked Immunosorbent Assay
William Jordan

Immunoblotting Techniques
Barbara Magi and Sabrina Liberatori

Epitope Mapping
Glenn E. Morris

Generation and Testing of Immunoaffinity Columns
Weilin L. Shelver

Immunocapture-Polymerase Chain Reaction
Vincent Mulholland

Magnetic Cell Sorting
Maciej Zborowski and Jeffrey J. Chalmers

Cell Enrichment and Immunochemical Staining
Oscar R. Lara and Jeffrey J. Chalmers


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