Immunochemical Protocols / Edition 3 by Robert Burns | 9781588292742 | Hardcover | Barnes & Noble
Immunochemical Protocols / Edition 3

Immunochemical Protocols / Edition 3

by Robert Burns
     
 

ISBN-10: 1588292746

ISBN-13: 9781588292742

Pub. Date: 12/13/2004

Publisher: Springer-Verlag New York, LLC

This much anticipated third edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques . It covers the breadth of relevant established methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and

Overview

This much anticipated third edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques . It covers the breadth of relevant established methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including conventional and recombinant antibodies, fusion proteins and their various conjugates. This book will open the door to a new generation of immunochemical reagents with exciting possibilities.

Product Details

ISBN-13:
9781588292742
Publisher:
Springer-Verlag New York, LLC
Publication date:
12/13/2004
Series:
Methods in Molecular Biology Series, #295
Edition description:
3rd ed. 2005
Pages:
318
Product dimensions:
6.10(w) x 9.25(h) x 0.03(d)

Table of Contents

Immunization Strategies for Antibody Production
Robert Burns

Synthetic Peptides as Antigens for Antibody Production
David C. Hancock and Nicola J. O'Reilly

Production of Polyclonal Antibodies in Rabbits
David C. Hancock and Nicola J. O'Reilly

Making Hybridomas
Robert Burns

Growing Hybridomas
Gary Entrican and Gareth Young

Phage Display Vectors for the In Vitro Generation of Human Antibody Fragments
Michael Hust and Stefan Dübel

Purification of Antibodies and Preparation of Antibody Fragments
Graham D. Kemp

Conjugation of Antibodies to Alkaline Phosphatase
G. Brian Wisdom

Conjugation of Antibodies to Horseradish Peroxidase
G. Brian Wisdom

Conjugation of Antibodies to Fluorescein or Rhodamine
G. Brian Wisdom

Conjugation of Antibodies to Biotin
James D. Hirsch and Rosaria P. Haugland

Preparation of Colloidal Gold Probes
David Hughes

Immunogold Probes in Light Microscopy
David Hughes

Immunoelectron Microscope Techniques in Plant Virus Diagnosis
Daphne M. Wright

Dual-Antibody Sandwich Enzyme-Linked Immunosorbent Assay
William Jordan

Competitive Enzyme-Linked Immunosorbent Assay
William Jordan

Immunoblotting Techniques
Barbara Magi and Sabrina Liberatori

Epitope Mapping
Glenn E. Morris

Generation and Testing of Immunoaffinity Columns
Weilin L. Shelver

Immunocapture-Polymerase Chain Reaction
Vincent Mulholland

Magnetic Cell Sorting
Maciej Zborowski and Jeffrey J. Chalmers

Cell Enrichment and Immunochemical Staining
Oscar R. Lara and Jeffrey J. Chalmers

Index

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