Immunochemical Protocols / Edition 3

Immunochemical Protocols / Edition 3

by Robert Burns
     
 

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ISBN-10: 1617374660

ISBN-13: 9781617374661

Pub. Date: 11/10/2010

Publisher: Springer-Verlag New York, LLC

This new third edition of a much praised classic has been updated and revised to provide both basic methods for producing and using antibodies and advanced techniques required by today's state-of-the art investigators. The basic sections of the book stress the first principles of antibody production and guide the reader through the processes and pitfalls of

Overview

This new third edition of a much praised classic has been updated and revised to provide both basic methods for producing and using antibodies and advanced techniques required by today's state-of-the art investigators. The basic sections of the book stress the first principles of antibody production and guide the reader through the processes and pitfalls of immunochemistry. The advanced sections focus on specific applications and cover the major immunochemical techniques currently used in laboratories. New material includes techniques for making new monoclonal cell lines, for producing good quality monoclonal antibodies, immunocapture PCR, immunoaffinity purification, and immunoelectron microscopy in plant virus diagnosis. The authors provide full details of methods for antigen selection and preparation, antibody production, and reagent manufacture. The prools follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Up-to-date and highly practical, Immunochemical Prools, Third Edition, takes both novice and experienced researchers from project initiation to final test format with a broad array of prools suited to immediate use in the laboratory, as well as methods that can be successfully applied to entirely new systems and processes.

Product Details

ISBN-13:
9781617374661
Publisher:
Springer-Verlag New York, LLC
Publication date:
11/10/2010
Series:
Methods in Molecular Biology Series , #295
Edition description:
Softcover reprint of hardcover 3rd ed. 2005
Pages:
318
Product dimensions:
6.10(w) x 9.00(h) x 0.80(d)

Table of Contents

Immunization Strategies for Antibody Production
Robert Burns

Synthetic Peptides as Antigens for Antibody Production
David C. Hancock and Nicola J. O'Reilly

Production of Polyclonal Antibodies in Rabbits
David C. Hancock and Nicola J. O'Reilly

Making Hybridomas
Robert Burns

Growing Hybridomas
Gary Entrican and Gareth Young

Phage Display Vectors for the In Vitro Generation of Human Antibody Fragments
Michael Hust and Stefan Dübel

Purification of Antibodies and Preparation of Antibody Fragments
Graham D. Kemp

Conjugation of Antibodies to Alkaline Phosphatase
G. Brian Wisdom

Conjugation of Antibodies to Horseradish Peroxidase
G. Brian Wisdom

Conjugation of Antibodies to Fluorescein or Rhodamine
G. Brian Wisdom

Conjugation of Antibodies to Biotin
James D. Hirsch and Rosaria P. Haugland

Preparation of Colloidal Gold Probes
David Hughes

Immunogold Probes in Light Microscopy
David Hughes

Immunoelectron Microscope Techniques in Plant Virus Diagnosis
Daphne M. Wright

Dual-Antibody Sandwich Enzyme-Linked Immunosorbent Assay
William Jordan

Competitive Enzyme-Linked Immunosorbent Assay
William Jordan

Immunoblotting Techniques
Barbara Magi and Sabrina Liberatori

Epitope Mapping
Glenn E. Morris

Generation and Testing of Immunoaffinity Columns
Weilin L. Shelver

Immunocapture-Polymerase Chain Reaction
Vincent Mulholland

Magnetic Cell Sorting
Maciej Zborowski and Jeffrey J. Chalmers

Cell Enrichment and Immunochemical Staining
Oscar R. Lara and Jeffrey J. Chalmers

Index

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