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Immunocytochemical Methods and Protocols offers a powerful collection of step-by-step immunocytochemical methods that can be used in many biological and biomedical disciplines in both research and clinical settings. Each protocol includes troubleshooting tips and alternative procedures, as well as informative explanations of why certain steps are necessary, and comprehensive lists of reagents and suppliers aids not usually found in standard journal recipes. Among the especially valuable protocols presented are those geared to preparing antibodies for staining procedures, light microscopic analysis, FACS, electron microscopy, and confocal microscopy. There are also protocols for nucleic acid immunocytochemistry, electron microscopic evaluation of antibody labeling using colloidal gold, and the use of antibodies for the differential diagnosis of tumors. Special applications arising from recent improvements in equipment design and technology among them laser microbeam workstations and automated immunostainers are also thoroughly presented.