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Doody's Review ServiceReviewer: Richard D. Minshall, PhD (University of Illinois at Chicago College of Medicine)
Description: This second edition provides detailed protocols for immunocytochemical localization of tissue constituents. The book is well balanced and suited for basic and clinical research scientists. Antibody preparation and purification, tissue and cell fixation, and fluorescent- and enzyme-conjugated antibody detection by light, confocal, and electron microscopy, as well as FACS analysis and in situ hybridization, are all covered. Examples of several protocols are given, providing the novice and expert researcher in any field of biology or medicine detailed protocols and solutions to pitfalls to ensure successful antigen localization and tissue morphology assessment.
Purpose: The purpose is to provide detailed protocols and guidance for conducting an immunocytochemical examination of tissue and cell constituents. This book provides a means of doing a "first" experiment with some assurance of success. The protocols are detailed enough to follow for the novice experimentalist, yet informative enough for the experienced investigator to adapt a new technique to his/her specific research questions.
Audience: Research scientists, technicians, and students who study or wish to localize tissue constituent, usually a protein, in the context of the whole cell or organ are the intended audiences for this book. The editor has successfully compiled this book for the intended readers. The editor and contributors are credible.
Features: The book contains protocols for antibody purification and conjugation to detection systems, tissue preparation and fixation for light, fluorescence, confocal, and electron microscopy, as well as FACS analysis and in situ hybridization. The protocol style format is useful for a first experiment. The illustrations are attractive and informative. The book as a whole is nicely done.
Assessment: I find this a very useful and practical addition to all fields of biology and medicine. It is very user friendly and should be a great reference for those designing experiments and writing.