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Immunology

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More About This Textbook

Overview


Designed to reflect the challenges of practicing biomedical science today, The Fundamentals of Biomedical Science series combines essential basic science with insights into laboratory practice, demonstrating how an understanding of the biology of disease is coupled with the analytical approaches that lead to diagnosis. Assuming only a minimum of prior knowledge, the series reviews the full range of disciplines to which a biomedical scientist may be exposed, from microbiology to cytopathology to transfusion science.

A new volume in this exciting series, Immunology offers biomedical science students insights into the function of the immune system, the frontline of defense against pathological disease, and the diagnostic techniques used to identify associated malfunctions and disorders. By examining the key immunological principles and scientific basis of laboratory techniques--with a focus on the biomedical scientist's role in the diagnostic laboratory--the authors provide students with everything they need to know in order to prepare for specialist studies in immunology. Case studies cover current tests, the rationale behind their use, the technologies employed, the quality measures applied, and the ways in which clinicians interpret test results in order to help patients.

Immunology is enhanced by numerous examples and full color throughout. A Companion Website offers resources for students and instructors, including a fully interactive digital microscope with a range of cell and tissue images for examination.

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Product Details

  • ISBN-13: 9780199534968
  • Publisher: Oxford University Press, USA
  • Publication date: 8/20/2010
  • Series: Fundamentals of Biomedical Science Series
  • Edition description: New Edition
  • Pages: 176
  • Product dimensions: 7.40 (w) x 9.60 (h) x 0.80 (d)

Table of Contents

1 Introduction to the clinical immunology laboratory Don Henderson Henderson, Don 1

Introduction 1

1.1 Immunology 2

1.2 Immunology in biomedical science 3

Chapter Summary 5

Discussion Questions 5

2 Immunoglobulins Phillip A Whitfield A Whitfield, Phillip 6

Introduction 6

2.1 Immunoglobulins 6

2.2 The monoclonal gammopathies 12

2.3 Quantification of immunoglobulins 14

2.4 Cerebrospinal fluid and isoelectric focusing 33

2.5 Cryoglobulins 37

Chapter Summary 40

Further Reading 41

Discussion Questions 42

3 Allergy I J Ansotegui Ansotegui, I J 43

Introduction 43

3.1 Allergy terminology 43

3.2 Allergens 44

3.3 Allergy mechanisms 45

3.4 Clinical features of allergic diseases 46

3.5 Clinical diagnosis 48

3.6 Laboratory diagnosis 51

3.7 Allergen standardization 52

3.8 Cross-reactivity 53

3.9 Recombinant allergens 54

3.10 Microarray-based allergy testing 57

3.11 Quality assurance 57

3.12 Total IgE measurements 59

3.13 IgG antibodies 60

3.14 Flow cytometric analyses 61

3.15 Measurement of mediators released during allergic reactions 64

3.16 Mast cell tryptase 64

3.17 Eosinophil cationic protein (ECP) 66

Chapter Summary 67

Further Reading 68

Discussion Questions 69

4 Complement Paul Morgan Morgan, Paul 70

Introduction 70

4.1 Actions of complement 71

4.2 Regulation of complement 74

4.3 Complement deficiencies 75

4.4 Measurement of complement activity 79

4.5 Cl inhibitor deficiency 85

4.6 Autoantibodies against complement components and complexes 87

4.7 Complement allotyping 88

Chapter Summary 89

Further Reading 89

Discussion Questions 89

5 Autoimmune rheumatological disease Peter J Charles Charles, Peter J 90

Introduction 90

5.1 Systemic lupus erythematosus (SLE) 91

5.2 Rheumatoid arthritis (RA) 92

5.3 Scleroderma 94

5.4 Sjogren's syndrome 95

5.5 Polymyositis and dermatomyositis 96

5.6 Anti-phospholipid syndrome (APS) 96

5.7 Overlapping connective tissue diseases 97

5.8 Autoantibodies 98

Chapter Summary 106

Further Reading 107

Discussion Questions 107

6 Autoimmune kidney disease Peter J Charles Charles, Peter J 108

Introduction 108

6.1 Goodpasture's (anti-GBM) disease 109

6.2 Wegener's granulomatosis 109

6.3 Microscopic polyangiitis (MPA) 110

6.4 Autoantibodies in autoimmune kidney diseases 111

6.5 Aetiology and pathogenesis of ANCA antibodies 113

6.6 Methods of detection 114

Chapter Summary 117

Further Reading 118

Discussion Questions 118

7 Organ-specific autoimmunity Robert J Lock Lock, Robert J 119

Introduction 119

7.1 Autoimmune thyroid disease 121

7.2 Coeliac disease 127

7.3 Pernicious anaemia 136

7.4 Autoimmune endocrinopathies 139

Chapter Summary 148

Further Reading 149

Discussion Questions 149

8 Autoimmune liver diseases Edward Davies Davies, Edward 151

Introduction 151

8.1 Autoimmune hepatitis (AIH) 152

8.2 Autoimmune sclerosing cholangitis (ASC) 161

8.3 Primary biliary cirrhosis (PBC) 162

8.4 Overlap syndromes 168

8.5 Technical notes 170

Chapter Summary 173

Further Reading 173

Discussion Questions 173

9 Neuroimmunology S Jacob Jacob, S 174

Introduction 174

9.1 Epidemiology of paraneoplastic syndrome 175

9.2 Clinical features of paraneoplastic syndromes 175

9.3 Malignancies Commonly associated with PNS 182

9.4 Paraneoplastic neurological antibodies (PNA) 182

9.5 Well-characterized antibodies 188

9.6 Partially characterized antibodies 196

9.7 Antibodies with or without cancer 199

9.8 Treatment of paraneoplastic syndromes 202

9.9 Miscellaneous antibodies 203

Chapter Summary 208

Further Reading 208

Acknowledgements 209

Discussion Questions 209

10 Flow cytometry and primary immunodeficiency Dawn Barge Barge, Dawn 210

Introduction 210

10.1 Lymphocyte subsets 211

10.2 T cells 215

10.3 B cells 221

10.4 Natural killer (NK) cells 223

10.5 Additional antibodies 224

10.6 Inducible antigens 226

10.7 Intracellular staining 229

10.8 Functional assays 230

10.9 Neutrophil defects 231

Chapter Summary 234

Further Reading 235

Discussion Questions 235

11 Human immunodeficiency virus (HIV) Alison Cox Cox, Alison 236

Introduction 236

11.1 The human immunodeficiency virus 236

11.2 The life cycle of HIV 237

11.3 Transmission of HIV 239

11.4 Effects of HIV on the immune system 239

11.5 Clinical features of HIV infection 240

11.6 Progression to AIDS 241

11.7 Laboratory diagnosis of HIV infection 242

11.8 Treatment regimens for HIV-1 infections 246

11.9 Prevention of HIV transmission and infection 250

Chapter Summary 250

Discussion Questions 251

References 253

Glossary 257

Abbreviations 261

Hints and tips for discussion questions 263

Index 269

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