In Situ Hybridization in Electron Microscopy

In Situ Hybridization in Electron Microscopy

by Gerard Morel, Annie Cavalier, Lynda Williams
     
 

ISBN-10: 0849300444

ISBN-13: 9780849300448

Pub. Date: 01/30/2001

Publisher: Taylor & Francis

This book demonstrates the scope that electron microscope in situ hybr idization can offer in characterizing and visualizing specific nucleic acid sequences in tissues and at the subcellular level. In situ hybri dization is a technique that allows the visualization of specific DNA/ RNA sequences in individual cells in tissue section, single cells, or chromosome

Overview

This book demonstrates the scope that electron microscope in situ hybr idization can offer in characterizing and visualizing specific nucleic acid sequences in tissues and at the subcellular level. In situ hybri dization is a technique that allows the visualization of specific DNA/ RNA sequences in individual cells in tissue section, single cells, or chromosome preparations, and is an especially important method for stu dying DNA and RNA in heterogeneous cell populations. This book covers methods in in situ hybridization using electron microscopy.

Product Details

ISBN-13:
9780849300448
Publisher:
Taylor & Francis
Publication date:
01/30/2001
Series:
Methods in Visualization Series
Pages:
472
Product dimensions:
7.20(w) x 10.20(h) x 1.20(d)

Table of Contents

General Introduction
Abbreviations
Probes
Principles of Methodology
Sample Preparation
Post-Embedding Technique
Pre-Embedding Technique
Frozen Tissue Technique
Semithin Sections
Controls and Problems
Examples of Results
Appendices
Equipment
Reagents
Glossary
Index

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