In Situ Hybridization in Electron Microscopy

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Overview

In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization of genes:
° hybridization on ultrathin sections of tissue embedded in hydrophilic resin (post-embedding method)
° hybridization prior to embedding (pre-embedding)
° hybridization on ultrathin sections of frozen tissue (frozen tissue method).
For each technique, the different stages are described in detail: the preparation of tissue, pretreatment, hybridization, and visualization of the hybridization products.

The book combines theory and practice, starting with the basic principles, then breaking down the experimental process into successive steps illustrated by numerous diagrams, detailed protocols, and tables. This is all done in a format that uses parallel columns to convey useful comments next to the theory and practical details alongside each stage of the protocol. Additionally, the summary tables provide the criteria for choosing the probe type and technique, and a detailed index aids in the search for information. in situ Hybridization In Electron Microscopy is an essential companion for applying these methods at the electron microscopic level.

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Editorial Reviews

Booknews
Provides techniques and protocols for visualizing specific nucleic acid sequences in tissue and single-cell preparations through electron microscopy. Combines theory and practice, starting with basic principles, then breaks down the experimental process into successive steps illustrated by diagrams, protocols, and tables for choosing the right protocol. Information is given in parallel columns to convey practical comments and advice. Coverage encompasses tissue preparation, section preparation, pretreatment, hybridization, controls and problems, and protocol types. Separate appendices provide information on the preparation of equipment and reagents. For experienced and novice researchers. Morel is affiliated with the National Center of Scientific Research, France. Annotation c. Book News, Inc., Portland, OR (booknews.com)
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Product Details

  • ISBN-13: 9780849300448
  • Publisher: CRC Press
  • Publication date: 1/30/2001
  • Series: Methods in Visualization Series
  • Pages: 472
  • Product dimensions: 7.20 (w) x 10.20 (h) x 1.20 (d)

Table of Contents

General Introduction
Abbreviations
Probes
Principles of Methodology
Sample Preparation
Post-Embedding Technique
Pre-Embedding Technique
Frozen Tissue Technique
Semithin Sections
Controls and Problems
Examples of Results
Appendices
Equipment
Reagents
Glossary
Index

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