In Situ Hybridization in Electron Microscopy

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This book demonstrates the scope that electron microscope in situ hybr idization can offer in characterizing and visualizing specific nucleic acid sequences in tissues and at the subcellular level. In situ hybri dization is a technique that allows the visualization of specific DNA/ RNA sequences in individual cells in tissue section, single cells, or chromosome preparations, and is an especially important method for stu dying DNA and RNA in heterogeneous cell populations. This book covers methods in in situ hybridization using electron microscopy.

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Editorial Reviews

Provides techniques and protocols for visualizing specific nucleic acid sequences in tissue and single-cell preparations through electron microscopy. Combines theory and practice, starting with basic principles, then breaks down the experimental process into successive steps illustrated by diagrams, protocols, and tables for choosing the right protocol. Information is given in parallel columns to convey practical comments and advice. Coverage encompasses tissue preparation, section preparation, pretreatment, hybridization, controls and problems, and protocol types. Separate appendices provide information on the preparation of equipment and reagents. For experienced and novice researchers. Morel is affiliated with the National Center of Scientific Research, France. Annotation c. Book News, Inc., Portland, OR (
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Product Details

  • ISBN-13: 9780849300448
  • Publisher: Taylor & Francis
  • Publication date: 1/30/2001
  • Series: Methods in Visualization Series
  • Pages: 472
  • Product dimensions: 7.20 (w) x 10.20 (h) x 1.20 (d)

Table of Contents

General Introduction
Principles of Methodology
Sample Preparation
Post-Embedding Technique
Pre-Embedding Technique
Frozen Tissue Technique
Semithin Sections
Controls and Problems
Examples of Results

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