In Situ Hybridization Techniques for the Brain

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Written by molecular biologists and neurobiologists, this volume in the Handbook Series provides basic step-by-step protocols in addition to specialist techniques to support those practising in situ hybridization, a powerful histochemical technique which is now widely used in neurobiology. The first part of the manual concentrates on basic procedures, eg, which probe and label to select, equipment, relevant molecular biological techniques, hazards, probe preparation and labelling, tissue preparation, and ...
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Overview

Written by molecular biologists and neurobiologists, this volume in the Handbook Series provides basic step-by-step protocols in addition to specialist techniques to support those practising in situ hybridization, a powerful histochemical technique which is now widely used in neurobiology. The first part of the manual concentrates on basic procedures, eg, which probe and label to select, equipment, relevant molecular biological techniques, hazards, probe preparation and labelling, tissue preparation, and conditions for hybridization. Specialist procedures including quantitative analysis of data, reverse transcription PCR, working with human tissue, and double label methods are presented in subsequent sections. In Situ Hybridization Techniques for the Brain is, therefore, an essential reference source for all those with research interests in neuroanatomy, histopathology and/or neurohistochemistry, and will support users in both the preparation and successful completion of the procedures in their own laboratory.

Describes molecular biological techniques, combined immunocytochemistry, quantitative analysis & interpretation.

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Product Details

Table of Contents

Contributors
Preface for IBRO Handbook Series
Preface
1 A Survey of the in Situ Hybridization Technique 1
2 Molecular Biological Techniques for in Situ Hybridization Involving the Use of Bacterial Cells 31
3 In Situ Hybridization with [superscript 35]S-Labelled Oligonucleotides 45
4 In Situ Hybridization with [superscript 35]S- and Digoxigenin-Labeled RNA Probes 79
5 Combined Immunocytochemistry and in Situ Hybridization 111
6 The Use of Human Post-Mortem Brain Tissue for in Situ Hybridization: Practical Considerations 119
7 Production of Labeled cRNA Probes Useful for in Situ Hybridization Histochemistry without Vector Cloning 133
8 Quantitative Analysis and Interpretation of Data for in Situ Hybridization at the Cellular Level 141
Appendix List of Suppliers 151
Index 157
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