In Vitro Toxicity Testing Protocols / Edition 1

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1995 Spiralbound New. this is spiral bound: BRAND NEW(NEVER OPENED)/slightly shelf-aged to cover-No international shipping available[GL5] 346 p. Contains: Illustrations. Methods ... in Molecular Biology (Paperback), 43. Audience: General/trade. Read more Show Less

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Overview

In Vitro Toxicity Testing Prools is a collection of proven, state-of-the-art methods that toxicologists can use in carrying out laboratory studies. The book's expert contributors offer a variety of efficient in vitro toxicology techniques that can be applied regularly in your lab, or can serve as the fundamental basis for the establishment of novel techniques in working laboratories. All the procedures are highly reliable.

Also included are ex vivo techniques, whose development embraces the twin aims of reduction and refinement of the use of laboratory animals; the editors believe this is an important step toward the ethical treatment of laboratory animals.

In keeping with the successful format of preceding volumes of the Methods in Molecular Biology series, concise, easy-to-follow, step-by-step prools are provided for each technique, together with extensive notes that cover troubleshooting and offer special tips to ensure the successful completion of your experiments.

The book contains black-and-white illustrations.

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Editorial Reviews

Doody's Review Service
Reviewer: Roberto A. Matamoros, DVM, PhD (University of Illinois at Chicago College of Medicine)
Description: This book provides an excellent presentation of the recent advances made in in vitro toxicology. Contributing authors place these advances in a meaningful perspective and present the material in easy-to-follow protocols.
Purpose: These protocols are designed to provide a collection of proven methods on in vitro toxicity testing. They present the scientific aspects of this complex and important area of toxicology and will be a valuable source of information for all those working in this field.
Audience: This book will serve the needs of scientists in the field of regulatory and research toxicology, including toxicologists, pathologists, and pharmacologists in industry, medicine, contract laboratories, and academia.
Features: This book presents the material with brevity, minimal use of jargon, and accuracy. The quality and pertinence of illustrations are appropriate. It presents practical references including recent publications. At the end of each chapter there is a useful section of notes with comments that cover troubleshooting and offer tips on how to get the best results from this techniques.
Assessment: This is a very good reference book for scientists in the field of in vitro toxicology. The authors succeeded in their efforts to fill the need for current knowledge on this subject. The introductory chapter on alternative tests for evaluating toxicity is especially useful in the areas of compound development, validation issues, and opportunities for the future in this important field of toxicology.
Roberto A. Matamoros
This book provides an excellent presentation of the recent advances made in in vitro toxicology. Contributing authors place these advances in a meaningful perspective and present the material in easy-to-follow protocols. These protocols are designed to provide a collection of proven methods on in vitro toxicity testing. They present the scientific aspects of this complex and important area of toxicology and will be a valuable source of information for all those working in this field. This book will serve the needs of scientists in the field of regulatory and research toxicology, including toxicologists, pathologists, and pharmacologists in industry, medicine, contract laboratories, and academia. This book presents the material with brevity, minimal use of jargon, and accuracy. The quality and pertinence of illustrations are appropriate. It presents practical references including recent publications. At the end of each chapter there is a useful section of notes with comments that cover troubleshooting and offer tips on how to get the best results from this techniques. This is a very good reference book for scientists in the field of in vitro toxicology. The authors succeeded in their efforts to fill the need for current knowledge on this subject. The introductory chapter on alternative tests for evaluating toxicity is especially useful in the areas of compound development, validation issues, and opportunities for the future in this important field of toxicology.
Booknews
A collection of techniques that toxicologists can apply in the lab or use as the basis for establishing new methods. Includes some ex vivo techniques to help reduce and refine the use of lab animals. Sections focus on: target organ toxicity, general and topical toxicity, irritancy testing, immunotoxicity, carcinogenesis, and submammalian and subvertebrate models for teratogenicity testing. Comb-binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)

4 Stars! from Doody
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Product Details

  • ISBN-13: 9780896032828
  • Publisher: Springer-Verlag New York, LLC
  • Publication date: 5/4/1995
  • Series: Methods in Molecular Biology Series , #43
  • Edition description: SPIRAL
  • Edition number: 1
  • Pages: 346
  • Product dimensions: 6.60 (w) x 8.90 (h) x 1.00 (d)

Table of Contents

Alternative Method of Assessing Toxicity. Target Organ Toxicity: LLC-RK1 Cell Screening Test for Nephrotoxicity. Preparation and Use of Cultured Astrocytes for Assay of Gliotoxicity. Human Thyroid Culture. Thyroid Follicular Cells in Monolayer Culture: In Vitro Models for Thyroid Toxicity Testing. Dust Toxicity in Rat Alveolar Macrophage Cultures. Hepatoma Cell Cultures as In Vitro Models for Hepatotoxicity. Cytotoxicity, DNA Fragmentation, and DNA Repair Synthesis in Primary Human Hepaytes. Model Cavity Method. Human Esophageal Culture. The Application of In Vitro Models of Anterior Pituitary Function in Toxicity Testing. The Application of In Vitro Models of Hypothalamic Function in Toxicity Testing. General and Topical Toxicity: The FRAME Cytotoxicity Test (Kenacid Blue). Allium Test. Measurement of Cell Membrane Toxicity by Means of 2-Deoxy-D-Glucose. MTT Assays. V79 Cytotoxicity Test for Membrane Damage. SIRC Cytotoxicity Test for Membrane Damage. Rabbit Articular Chondrocyte Functional Toxicity Test. Balb/c 3T3 Cytotoxicity Test. The Pollen Tube Growth Test. Irritancy Testing: HET-CAM Test. The Use of the Bovine Isolated Cornea as a Possible In Vitro Test for Occular Irritancy. Cytotoxicity in an Anchorage-Independent Fibroblast Cell Line Measured by a Combination of Fluorescent Dyes. Cell Culture Phototoxicity Test. Immunotoxicity: The Murine Local Lymph Node Assay. Polymorphonuclear Leukocytes Locomotion. Immune Function Assays. Carcinogenesis: The DNA Alkaline Unwinding Genotoxicity Test. Measurement of Unscheduled DNA Synthesis In Vitro Using Primary Rat Hepayte Cultures. Gene Mutation Assays in Mammalian Cells. Measurement of Chromosome Aberrations In Vitro Using Human Peripheral Blood Lymphocytes. Mutation Assays in Bacteria. Submammalian and Subvertebrate Models for Teratogenicity Testing: Chick Embryrotoxicity Screening Test (CHEST I and II). Frog Embryo Teratogenesis Assay: Xenopus (FETAX). The Drosophila melanogaster Assay. The Hydra attenuata Assay. Index.

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