In Vivo Optical Imaging of Brain Function, Second Edition / Edition 2

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These are exciting times for the field of optical imaging of brain function. Rapid developments in theory and technology continue to considerably advance understanding of brain function. Reflecting changes in the field during the past five years, the second edition of In Vivo Optical Imaging of Brain Function describes state-of-the-art techniques and their applications for the growing field of functional imaging in the live brain using optical imaging techniques.

New in the Second Edition:

  • Voltage-sensitive dyes imaging in awake behaving animals
  • Imaging based on genetically encoded probes
  • Imaging of mitochondrial auto-fluorescence as a tool for cortical mapping
  • Using pH-sensitive dyes for functional mapping
  • Modulated imaging
  • Calcium imaging of neuronal activity using 2-photon microscopy
  • Fourier approach to optical imaging
  • Fully updated chapters from the first edition

Leading Authorities Explore the Latest Techniques

Updated to reflect continuous development in this emerging research area, this new edition, as with the original, reaches across disciplines to review a variety of non-invasive optical techniques used to study activity in the living brain. Leading authorities from such diverse areas as biophysics, neuroscience, and cognitive science present a host of perspectives that range from a single neuron to large assemblies of millions of neurons, captured at various temporal and spatial resolutions. Introducing techniques that were not available just a few years ago, the authors describe the theory, setup, analytical methods, and examples that highlight the advantages of each particular method.

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Product Details

  • ISBN-13: 9781420076844
  • Publisher: CRC Press
  • Publication date: 5/6/2009
  • Series: Frontiers in Neuroscience Series
  • Edition description: New Edition
  • Edition number: 2
  • Pages: 428
  • Product dimensions: 6.00 (w) x 9.30 (h) x 1.10 (d)

Table of Contents

Optical Imaging of Brain Activity In Vivo Using Genetically Encoded Probes, M. Wachowiak and T. Knöpfel

Two-Photon Functional Imaging of Neuronal Activity, F. Helmchen

In Vivo Two-Photon Laser Scanning Microscopy with Concurrent Plasma-Mediated Ablation: Principles and Hardware Realization, P.S. Tsai and D. Kleinfeld

MPScope 2.0: A Computer System for Two-Photon Laser Scanning Microscopy with Concurrent Plasma-Mediated Ablation and Electrophysiology, Q.-T. Nguyen, E.M. Dolnick, J. Driscoll, and D. Kleinfeld

In Vivo Observations of Rapid Scattered Light Changes Associated with Neurophysiological Activity, D.M. Rector, X. Yao, R.M. Harper, and J.S. George

Imaging the Brain in Action: Real-Time Voltage-Sensitive Dye Imaging of Sensorimotor Cortex of Awake Behaving Mice, I. Ferezou, F. Matyas, and C.C.H. Petersen

Flavoprotein Fluorescence Imaging of Experience-Dependent Cortical Plasticity in Rodents, K. Shibuki, R. Hishida, M. Tohmi, K. Takahashi, H. Kitaura, and Y. Kubota

Functional Imaging with Mitochondrial Flavoprotein Autofluorescence: Theory, Practice, and Applications, T.R. Husson and N.P. Issa

Visualizing Adult Cortical Plasticity Using Intrinsic Signal Optical Imaging, R.D. Frostig and C.H. Chen-Bee

Fourier Approach for Functional Imaging, V.A. Kalatsky

Optical Imaging of Neuronal Activity in the Cerebellar Cortex Using Neutral Red, T.J. Ebner, G. Chen, and W. Gao

Quantitative In Vivo Imaging of Tissue Absorption, Scattering, and Hemoglobin Concentration in Rat Cortex Using Spatially-Modulated Structured Light, D.J. Cuccia, D. Abookasis, R.D. Frostig, and B.J. Tromberg

Intraoperative Optical Imaging, K.C. Brennan and A.W. Toga

Noninvasive Imaging of Cerebral Activation with Diffuse Optical Tomography, T.J. Huppert, M.A. Franceschini, and D.A. Boas

Fast Optical Signals: Principles, Methods, and Experimental Results, G. Gratton and M. Fabiani

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