Few research efforts have focused on factors associated with bisexual and lesbian partner violence. The present study examined the role of internalized homophobia, self-esteem, income levels, and educational levels and the use of violence to inform on the factors that may influence bisexual and lesbian partner violence. A sample size of 134 women over the age of 18 who self-identified as bisexual, mostly lesbian, or lesbian were recruited using a non-probability sampling convenience design and multiple recruitment methods. The web-based questionnaire survey was formatted for Internet delivery and hosted through surveymonkey.com using demographic questions, the Internalized Homophobia Scale (IHP), the Revised Conflict Tactics Scales (CTS2), and the Rosenberg Self-esteem Scale (RSE) as measures. Over half of the participants reported never having experienced partner violence in their same-gender relationships. Approximately one-third of the sample had been victimized by same-gender partner violence. This finding falls within the range of prevalence estimates for violence in bisexual and lesbian women relationships and is consistent with estimates for heterosexual partner violence. More than half of those reporting partner violence had both perpetrated and been victimized by a same-gender partner. The primary finding in this study was that internalized homophobia is a significant predictor of the use of physical violence and sexual coercion in the relationships of bisexual, mostly lesbian and lesbian relationships. Those who reported low levels of internalized homophobia were significantly less likely to be involved in physical violence or sexual coercion with their same-gender partners. A secondary finding was that low levels of formal education were also a predictor of the number of aggressive acts on partners. Participants with less than a college education were more than twice as likely to commit physically violent or sexually coercive acts as those with advanced or graduate degrees. Self-esteem and income levels were not significantly associated with violence. An unexpected finding concerned the overlapping of perpetrator and victim status. These results provide support for theoretical models of partner violence that consider the effects of internalized homophobia as stressors, which may be experienced by either person within the relationship, increasing the risk of violence within the relationship.