Intracellular pH and its Measurement

by Arnost Kotyk, Jan Slavik

Overview

This volume introduces a summary of all the techniques used to estimate pH reliably. Emphasis is placed on the techniques that provide the most reliable and detailed data. The role of cell pH is explained with special emphasis on enzymology and membrane transport and bioenergetics. This book was written especially for molecular biologists, biochemists and biophysicists.

Product Details

ISBN-13:	9780849349164
Publisher:	Taylor & Francis
Publication date:	04/30/1989
Pages:	192
Product dimensions:	7.00(w) x 10.00(h) x (d)

About the Author

Arnost Kotyk and Jan Slavik

Table of Contents

The Concept of pH, Hydrolysis, and Buffers. pH in Biological Systems. Methods of Determining Intracellular pH with Electrodes. Assays of Intracellular pH Using Chemical Probes: Principles of pH Indicator Response. Assays of Intracellular pH Using Chemical Probes: Distribution Techniques. Assays of Intracellular pH Using Chemical Probes: Absorption Spectroscopy. Assays of Intracellular pH Using Chemical Probes: Fluorescence Spectroscopy. Assays of Intracellular pH Using Chemical Probes: Nuclear Magnetic Resonance Spectroscopy. The Choice of the Assay Method. Heterogeneity of Intracellular pH. Index.

Editorial Reviews

Two physiologists from the Czechoslovak Academy of Sciences, Prague, summarize the current knowledge of measuring pH inside cells. Kotyk has published and lectured extensively worldwide. Slavik invented the double fluorescence method, now the most widely used in general laboratories to measure local pH values. The bulk of the book is devoted to assaying with chemical probes, which is discussed in terms of indicator response, distribution techniques, absorption spectroscopy, fluorescence spectroscopy, and nuclear magnetic resonance spectroscopy. Chapters also cover the concept of pH, hydrolysis, and buffers, pH in biological systems, measurement with electrodes, the choice of assay method, and the heterogeneity of intercellular pH. Printed on acidic paper. Annotation c. Book News, Inc., Portland, OR (booknews.com)

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