Introduction to Enzyme and Coenzyme Chemistry / Edition 3

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Looking at enzymes from an organic chemistry perspective, this updated reference includes information on recent advances in our understanding of enzyme action; topical examples to illustrate key points; two-color figures of the active sites of enzymes discussed in the text to illustrate the interplay between enzyme structure and function; and end-of-chapter problems to allow readers to check their understanding of the material. This concise but comprehensive guide is essential for undergraduate and postgraduate students of organic, bio-organic, and medicinal chemistry, chemical biology, biochemistry, and biotechnology.

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Editorial Reviews

From the Publisher
“Summing Up: Recommended.  Lower-and upper-division undergraduates.”  (Choice, 1 April 2013)
Doody's Review Service
Reviewer: Gene A. Homandberg, PhD (Rush Medical College of Rush University)
Description: This book is intended to be an introduction to organic chemical descriptions of the major types of enzyme mechanisms. It begins with chapters on enzymes as proteins, enzyme catalysis, and study methods and continues with discussion of the six major types of enzyme mechanisms. The last chapter discusses nonenzymatic catalysis, as mediated by catalytic RNA and catalytic antibodies.
Purpose: This book is an attempt to present the major types of mechanisms together and to emphasize the chemical concepts. This is a worthwhile purpose for organic chemistry students, although there are many enzyme and general biochemistry books that do present mechanisms in sufficient detail and also discuss and more completely illustrate the role of protein structure. Because of this emphasis, this book would be useful to emphasize the features and relevance of biologic catalysts in an organic chemistry course.
Audience: The audience is intended to be primarily biochemists, and especially students; however, the book would also be suitable for organic chemists or instructors wishing to expand their knowledge or explain the relevance of protein catalysts.
Features: This book has an attractive, easy to read format and features numerous illustrations, problem sets and suggested additional reading throughout.
Assessment: This book would be a worthwhile addition to the library of biochemistry students or instructors, because it does comprehensively present the major types of mechanisms in an easy-to-read and understand style. Although there are many other excellent enzyme books with a different emphasis, this book would still be quite useful.
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Product Details

  • ISBN-13: 9781119995944
  • Publisher: Wiley
  • Publication date: 8/21/2012
  • Edition description: New Edition
  • Edition number: 3
  • Pages: 290
  • Sales rank: 1,292,928
  • Product dimensions: 6.60 (w) x 9.50 (h) x 0.80 (d)

Meet the Author

Tim Bugg is Professor of Biological Chemistry in the Department of Chemistry, University of Warwick, UK.

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Table of Contents

Preface ix

Representation of Protein Three-Dimensional Structures x

1 From Jack Beans to Designer Genes 1

1.1 Introduction 1

1.2 The discovery of enzymes 1

1.3 The discovery of coenzymes 2

1.4 The commercial importance of enzymes in biosynthesis and biotechnology 3

1.5 The importance of enzymes as targets for drug discovery 5

2 All Enzymes Are Proteins 7

2.1 Introduction 7

2.2 The structures of the L—amino acids 7

2.3 The primary structure of polypeptides 9

2.4 Alignment of amino acid sequences 11

2.5 Secondary structures found in proteins 12

2.6 The folded tertiary structure of proteins 15

2.7 Enzyme structure and function 17

2.8 Metallo-enzymes 19

2.9 Membrane-associated Enzymes 20

2.10 Glycoproteins 21

3 Enzymes are Wonderful Catalysts 25

3.1 Introduction 25

3.2 A thermodynamic model of catalysis 27

3.3 Proximity effects 28

3.4 The importance of transition state stabilisation 31

3.5 Acid/base catalysis in enzymatic reactions 34

3.6 Nucleophilic catalysis in enzymatic reactions 37

3.7 The use of strain energy in enzyme catalysis 41

3.8 Desolvation of substrate and active site nucleophiles 42

3.9 Catalytic perfection 44

3.10 The involvement of protein dynamics in enzyme catalysis 44

4 Methods for Studying Enzymatic Reactions 47

4.1 Introduction 47

4.2 Enzyme purification 47

4.3 Enzyme kinetics 49

4.4 The stereochemical course of an enzymatic reaction 55

4.5 The existence of intermediates in enzymatic reactions 61

4.6 Analysis of transition states in enzymatic reactions 64

4.7 Determination of active site catalytic groups 67

5 Hydrolytic and Group Transfer Enzymes 72

5.1 Introduction 72

5.2 The peptidases 73

5.3 Esterases and lipases 85

5.4 Acyl transfer reactions in biosynthesis (coenzyme A) 86

5.5 Enzymatic phosphoryl transfer reactions 88

5.6 Adenosine 5-triphosphate (ATP) 93

5.7 Enzymatic glycosyl transfer reactions 95

5.8 Methyl group transfer: use of S-adenosyl methionine and tetrahydrofolate coenzymes for one-carbon transfers 99

6 Enzymatic Redox Chemistry 108

6.1 Introduction 108

6.2 Nicotinamide adenine dinucleotide-dependent dehydrogenases 110

6.3 Flavin-dependent dehydrogenases and oxidases 115

6.4 Flavin-dependent mono-oxygenases 120

6.5 CASE STUDY: Glutathione and trypanothione reductases 122

6.6 Deazaflavins and pterins 126

6.7 Iron-sulphur clusters 127

6.8 Metal-dependent mono-oxygenases 128

6.9 -Ketoglutarate-dependent dioxygenases 131

6.10 Non-heme iron-dependent dioxygenases 133

7 Enzymatic Carbon–Carbon Bond Formation 139

7.1 Introduction 139

Carbon–carbon bond formation via carbanion equivalents 140

7.2 Aldolases 140

7.3 Claisen enzymes 144

7.4 Assembly of fatty acids and polyketides 146

7.5 Carboxylases: Use of biotin 150

7.6 Ribulose bisphosphate carboxylase/oxygenase (Rubisco) 151

7.7 Vitamin K-dependent carboxylase 153

7.8 Thiamine pyrophosphate-dependent enzymes 155

Carbon–carbon bond formation via carbocation intermediates 158

7.9 Terpene cyclases 158

Carbon–carbon formation through radical intermediates 162

7.10 Phenolic radical couplings 163

8 Enzymatic Addition/Elimination Reactions 170

8.1 Introduction 170

8.2 Hydratases and dehydratases 171

8.3 Ammonia lyases 175

8.4 Elimination of phosphate and pyrophosphate 177

8.5 CASE STUDY: 5-Enolpyruvyl shikimate 3-phosphate (EPSP) synthase 180

9 Enzymatic Transformations of Amino Acids 185

9.1 Introduction 185

9.2 Pyridoxal 5-phosphate-dependent reactions at the -position 185

9.3 CASE STUDY: Aspartate aminotransferase 189

9.4 Reactions at the - and -positions of amino acids 192

9.5 Serine hydroxymethyltransferase 195

9.6 N-Pyruvoyl-dependent amino acid decarboxylases 195

9.7 Imines and enamines in alkaloid biosynthesis 196

10 Isomerases 200

10.1 Introduction 200

10.2 Cofactor-independent racemases and epimerases 200

10.3 Keto-enol tautomerases 203

10.4 Allylic isomerases 203

10.5 CASE STUDY: Chorismate mutase 206

11 Radicals in Enzyme Catalysis 211

11.1 Introduction 211

11.2 Vitamin B12-dependent rearrangements 211

11.3 The involvement of protein radicals in enzyme catalysis 214

11.4 S-adenosyl-methionine-dependent radical reactions 217

11.5 Biotin synthase and sulphur insertion reactions 219

11.6 Radical chemistry in DNA repair enzymes 221

11.7 Oxidised amino acid cofactors and quinoproteins 221

12 Non-Enzymatic Biological Catalysis 228

12.1 Introduction 228

12.2 Catalytic RNA 228

12.3 Catalytic antibodies 232

12.4 Synthetic enzyme models 238

Appendix 1: Cahn-Ingold-Prelog Rule for Stereochemical Nomenclature 243

Appendix 2: Amino Acid Abbreviations 245

Appendix 3: A Simple Demonstration of Enzyme Catalysis 246

Appendix 4: Answers to Problems 248

Index 255

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