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Introduction to Immunocytochemistry / Edition 3

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Overview

Now in its third edition, Introduction to Immunocytochemistry is an essential text for newcomers to the field, and will be a valuable reference for more experienced workers. Extensively expanded and updated, this volume covers both background theory and practical applications, including immunostaining methods using fluorescent, enzyme and gold labels.
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Editorial Reviews

Doody's Review Service
Reviewer: Meera R Hameed, MD (University of Medicine and Dentistry of New Jersey-NJMS)
Description: The third edition of this book has been reorganized and updated, with expanded sections on tissue and cytological preparations. New methods, such as multiple immunohistochemical staining are included. In 12 chapters, this concise book covers the entire range of immunohistochemistry from antibody production to the preparation of tissue to methods of visualization. The previous edition was published in 1997.
Purpose: The purpose is to provide enough theoretical and practical information pertaining to immunohistochemical methods and its applications and thus allow newcomers to understand and apply this technology. The objectives are worthwhile and the book meets them.
Audience: It is written for histotechnologists, students, residents, and practitioners. It is suitable for practicing pathologists, residents, researchers, and technologists.
Features: This book describes the basic principles and applications of immunohistochemistry including types and characteristics of antibodies, tissue fixation, frozen sections, cytological preparations, blood and bone marrow smears and cultured cells. The properties of various detection agents have been addressed in detail. Common problems such as nonspecific staining are identified and solutions are provided. The method section is well written and addresses the evolution of the various techniques including salient points in the variety of applications. Antibody specificities, importance of positive and negative controls, remedies for nonspecificity are also well addressed. In addition to routine immunostaining methods, there are two chapters describing various methods involving multiple immunostaining and immunohistochemistry for electron microscopy. There is a limited section on applications of immunohistochemistry and research related subjects including confocal microscopy, quantification, probe uses, etc., Finally an appendix section is given with chemical composition and details of various methods.
Assessment: This well written book serves as an excellent and practical guide for immunohistochemistry applications. Compared to other books in the field which predominantly focus on applications, this one concentrates on the technical aspects including troubleshooting. Thus, it is a very useful book for day-to-day operations in the laboratory. This third edition has updated sections on the various tissue and cell preparations and so it is justified to replace the previous edition.
E-Streams
"...Highly recommended for academic and special libraries. The informative content and high level of readability makes this a desirable item for collections with relevant programs"
October 2004

3 Stars from Doody
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Product Details

  • ISBN-13: 9781859962084
  • Publisher: Taylor & Francis
  • Publication date: 7/29/2003
  • Edition description: REV
  • Edition number: 3
  • Pages: 192
  • Product dimensions: 6.10 (w) x 9.10 (h) x 0.50 (d)

Table of Contents

CH 1. INTRODUCTION
CH 2. PRODUCTION OF ANTIBODIES
CH 3. PREPARATION OF TISSUE FOR IMMUNOCYTOCHEMISTRY
CH 4. VISUALISING THE END-PRODUCT OF REACTION
CH 5. NON-SPECIFIC STAINING DUE TO TISSUE FACTORS
CH 6. METHODS
CH 7. TESTING ANTIBODIES: SPECIFICITY AND ESSENTIAL CONTROLS
CH 8. INCREASING SENSITIVITY AND ENHANCING STANDARD METHODS
CH 9. MULTIPLE IMMUNOSTAINING
CH 10. IMMUNOCYTOCHEMISTRY FOR THE TRANSMISSION ELECTRON
CH 11. IN VITRO METHODS FOR TESTING ANTIGEN-ANTIBODY REACTIONS
CH 12. APPLICATIONS OF IMMUNOCYTOCHEMISTRY
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