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Doody's Review ServiceReviewer: Meera R Hameed, MD (University of Medicine and Dentistry of New Jersey-NJMS)
Description: The third edition of this book has been reorganized and updated, with expanded sections on tissue and cytological preparations. New methods, such as multiple immunohistochemical staining are included. In 12 chapters, this concise book covers the entire range of immunohistochemistry from antibody production to the preparation of tissue to methods of visualization. The previous edition was published in 1997.
Purpose: The purpose is to provide enough theoretical and practical information pertaining to immunohistochemical methods and its applications and thus allow newcomers to understand and apply this technology. The objectives are worthwhile and the book meets them.
Audience: It is written for histotechnologists, students, residents, and practitioners. It is suitable for practicing pathologists, residents, researchers, and technologists.
Features: This book describes the basic principles and applications of immunohistochemistry including types and characteristics of antibodies, tissue fixation, frozen sections, cytological preparations, blood and bone marrow smears and cultured cells. The properties of various detection agents have been addressed in detail. Common problems such as nonspecific staining are identified and solutions are provided. The method section is well written and addresses the evolution of the various techniques including salient points in the variety of applications. Antibody specificities, importance of positive and negative controls, remedies for nonspecificity are also well addressed. In addition to routine immunostaining methods, there are two chapters describing various methods involving multiple immunostaining and immunohistochemistry for electron microscopy. There is a limited section on applications of immunohistochemistry and research related subjects including confocal microscopy, quantification, probe uses, etc., Finally an appendix section is given with chemical composition and details of various methods.
Assessment: This well written book serves as an excellent and practical guide for immunohistochemistry applications. Compared to other books in the field which predominantly focus on applications, this one concentrates on the technical aspects including troubleshooting. Thus, it is a very useful book for day-to-day operations in the laboratory. This third edition has updated sections on the various tissue and cell preparations and so it is justified to replace the previous edition.