Introduction to Modern Liquid Chromatography / Edition 3

Introduction to Modern Liquid Chromatography / Edition 3

by Lloyd R. Snyder, John W. Dolan, Joseph J. Kirkland
     
 

The latest edition of the authoritative reference to HPLC

High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier

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Overview

The latest edition of the authoritative reference to HPLC

High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples.

This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as:

  • The basis of HPLC separation and the general effects of different experimental conditions
  • Equipment and detection
  • The column—the "heart" of the HPLC system
  • Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques
  • Computer simulation, qualitative and quantitative analysis, and method validation and quality control
  • The separation of large molecules, including both biological and synthetic polymers
  • Chiral separations, preparative separations, and sample preparation
  • Systematic development of HPLC separations—new to this edition
  • Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms

Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

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Product Details

ISBN-13:
9780470167540
Publisher:
Wiley
Publication date:
10/28/2009
Pages:
960
Sales rank:
920,584
Product dimensions:
7.20(w) x 10.00(h) x 1.90(d)

Table of Contents

Glossary of symbols and abbreviations

1 Introduction 1

2 Basic Concepts and the Control of Separation 19

3 Equipment 87

4 Detection 147

5 The Column 199

6 Reversed-Phase Chromatography for Neutral Samples 253

7 Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange Chromatography 303

8 Normal-Phase Chromatography 361

9 Gradient Elution 403

10 Computer-Assisted Method Development 475

11 Qualitative and Quantitative Analysis 499

12 Method Validation Michael Swartz Swartz, Michael Lloyd R. Synder Synder, Lloyd R. 531

13 Biochemical and Synthetic Polymer Separations Timothy Wehr Wehr, Timothy Carl Scandella Scandella, Carl Peter Schoenmakers Schoenmakers, Peter Lloyd R. Synder Synder, Lloyd R. 569

14 Enantiomer Separations Michael Lammerhofer Lammerhofer, Michael Nobert M. Maier Maier, Nobert M. Wolfgang Linder Linder, Wolfgang Lloyd R. Synder Synder, Lloyd R. 665

15 Preparative Separations Geoff Cox Cox, Geoff Lloyd R. Synder Synder, Lloyd R. 725

16 Sample Preparation Ronald Majors Majors, Ronald Lloyd R. Synder Synder, Lloyd R. 757

17 Troubleshooting 809

Appendix I Properties of HPLC Solvents 879

Appendix II Preparing Buffered Mobile Phases 887

Index 891

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