Introduction to Modern Liquid Chromatography / Edition 2

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Overview

"High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples." "This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics." Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

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Editorial Reviews

From the Publisher
"In summary, I would strongly recommend this book for anyone working with LC and LC/MS. It is a finely crafted introduction that adequately covers nearly every aspect of the science. The emphasis on basic principles and practical aspects ensures that it will be a useful reference for many years to come." (J Am Soc Mass Spectrom, 2011)

"This third edition is highly cross-referenced, so as to allow the reader to follow up on topics of special interest, or to clarify questions that may arise during reading. The third edition of Introduction to Modern Liquid Chromatography will continue to be the HPLC reference book for thousands of readers, either experienced workers who may wish to explore topics of his/her choice, or find an answer to specific problems, or beginners who would like to understand and know the possibilities offered by the technique." (Chemistry Journals, 11 April 2011)

"This classic text on liquid chromatography has been thoroughly updated through the addition of information on modern instrumentation, columns, and troubleshooting. It is a valuable resource for practicing chromatographers at all levels.." (Anal Bioanal Chem, 2011)

"In summary, I would strongly recommend this book for anyone working with LC and LC/MS. It is a finely crafted introduction that adequately covers nearly every aspect of the science. The emphasis on basic principles and practical aspects ensures that it will be a useful reference for any years to come." (American Society for Mass Spectrometry, 21 Januay 2011)

"The text is illustrated with many figures and tables originating from authors' own work or taken from the literature . . . both groups of readers will find in this book plenty of information and inspiration." (Journal of Separation Science, 1 June 2010)

"Following their highly successful second edition (1979), which appeared more than 30 years ago, Snyder (LC Resources) and Kirkland (Advanced Materials Technology) have teamed with Dolan (LC Resources) and additional collaborators to provide an excellent update of their earlier work." (CHOICE, July 2010)

"It is difficult in these times to ever call a scientific book a "bargain", but this truly is. The wealth of information contained in these almost 1000 pages is invaluable. The book is comprehensive, eminently readable and approachable, and highly useful for both the skilled chromatographer and those new to the technique. Everyone using a liquid chromatographic instrument would benefit from owning a copy." (JACS, 2010)

"It's current, clearly-indexed, well-referenced, and comprehensive. The inclusion of John Dolan as author has significantly expanded the sections on troubleshooting, and additional expert contributors have enhanced the coverage of the specialist chapters." - Bruce Hamilton posted on the Chromatography Forum

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Product Details

  • ISBN-13: 9780471038221
  • Publisher: Wiley, John & Sons, Incorporated
  • Publication date: 11/28/1979
  • Edition description: REV
  • Edition number: 2
  • Pages: 896
  • Product dimensions: 6.38 (w) x 9.39 (h) x 1.97 (d)

Meet the Author

Lloyd R. Snyder, PhD, is a Principal at LC Resources in Walnut Creek, California. He is the author or coauthor of several books including An Introduction to Separation Science, Introduction to Modern Liquid Chromatography, Second Edition, the bestselling Practical HPLC Method Development, Second Edition, and the comprehensive High-Performance Gradient Elution, all published by Wiley.

Joseph J. Kirkland, PhD, is Vice President of Research and Development for Advanced Materials Technology, Inc., and coauthor of Introduction to Modern Liquid Chromatography, Second Edition, Practical HPLC Method Development, Second Edition, and Modern Size-Exclusion Liquid Chromatography, Second Edition, all published by Wiley.

John W. Dolan, PhD, is a Principal at LC Resources. He is author of the popular "LC Troubleshooting" column in LCGC magazine and coauthor with Lloyd Snyder of Troubleshooting LC Systems and High-Performance Gradient Elution.

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Table of Contents

Glossary of symbols and abbreviations

1 Introduction 1

2 Basic Concepts and the Control of Separation 19

3 Equipment 87

4 Detection 147

5 The Column 199

6 Reversed-Phase Chromatography for Neutral Samples 253

7 Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange Chromatography 303

8 Normal-Phase Chromatography 361

9 Gradient Elution 403

10 Computer-Assisted Method Development 475

11 Qualitative and Quantitative Analysis 499

12 Method Validation Michael Swartz Swartz, Michael Lloyd R. Synder Synder, Lloyd R. 531

13 Biochemical and Synthetic Polymer Separations Timothy Wehr Wehr, Timothy Carl Scandella Scandella, Carl Peter Schoenmakers Schoenmakers, Peter Lloyd R. Synder Synder, Lloyd R. 569

14 Enantiomer Separations Michael Lammerhofer Lammerhofer, Michael Nobert M. Maier Maier, Nobert M. Wolfgang Linder Linder, Wolfgang Lloyd R. Synder Synder, Lloyd R. 665

15 Preparative Separations Geoff Cox Cox, Geoff Lloyd R. Synder Synder, Lloyd R. 725

16 Sample Preparation Ronald Majors Majors, Ronald Lloyd R. Synder Synder, Lloyd R. 757

17 Troubleshooting 809

Appendix I Properties of HPLC Solvents 879

Appendix II Preparing Buffered Mobile Phases 887

Index 891

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