Introduction to Plant Biotechnology

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Editorial Reviews

From The Critics
Expanded from the original 368 pages, this edition updates coverage of recombinant DNA technology and adds material on plant tissue culture in the areas of mutagenesis, genomics, bioinformatics, gene transfer methods, and transgenics in crop improvement. Emphasizing application of techniques, Chawla (genetics and plant breeding, G.B. Pant U. of Agriculture & Technology, Pantnagar, India) provides protocols for lab exercises. Includes the preface to the 2000 edition, a chapter on intellectual property rights, a glossary, and technical appendices. Distributed in the US by Enfield. Annotation c. Book News, Inc., Portland, OR (booknews.com)
Booknews
A textbook for graduate or undergraduate courses in various departments in which Chawla (genetics and plant breeding, G.B. Pant U. of Agriculture and Technology, Pantnagar, India) presents the basic aspects of gene and genome organization in plant cells, basic tissue culture techniques, the fundamentals of cloning, approaches to gene transfer, and molecular markers. He also includes a chapter on intellectual property rights. Distributed in the US by Enfield. Annotation c. Book News, Inc., Portland, OR (booknews.com)
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Product Details

  • ISBN-13: 9781578082285
  • Publisher: Science Publishers
  • Publication date: 6/1/2002
  • Edition description: REV
  • Edition number: 2
  • Pages: 540

Table of Contents

Preface to the Second Edition
Preface to the First Edition
Abbreviations
Pt. I Plant Tissue Culture
1 Introduction 3
2 Laboratory Organization 10
3 Nutrition Medium 14
4 Sterilization Techniques 23
5 Types of Culture 27
6 Micropropagation 39
7 Cell Suspension and Secondary Metabolites 57
8 In Vitro Production of Haploids 74
9 Protoplast Isolation and Fusion 87
10 Somaclonal Variation 110
11 Germplasm Storage and Cryopreservation 123
Pt. II Genetic Material and Its Organization
12 Genetic Material 133
13 Organization of DNA and Gene Expression 145
Pt. III Recombinant DNA Technology
14 Basic Techniques 173
15 Gene Cloning: Cutting and Joining DNA Molecules 189
16 Gene Cloning: Vectors 201
17 Gene Cloning: cDNA and Genomic Cloning and Analysis of Cloned DNA Sequences 225
18 Polymerase Chain Reaction 248
19 In Vitro Mutagenesis 269
20 Transposon Genetic Elements and Gene Tagging 290
21 Gene Isolation 314
22 Molecular Markers and Marker-Assisted Selection 329
23 Gene Transfer in Plants 359
24 Transgenics in Crop Improvement 396
25 Genomics 428
26 Bioinformatics 459
27 Intellectual Property Rights 467
Appendix 484
References 487
Glossary 501
Author index 523
Subject index 528
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