The diverse applications in this volume range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, and for studying channel regulation.
Our reader reviews allow you to share your comments on titles you liked,
or didn't, with others. By submitting an online review, you are representing to
Barnes & Noble.com that all information contained in your review is original
and accurate in all respects, and that the submission of such content by you
and the posting of such content by Barnes & Noble.com does not and will not
violate the rights of any third party. Please follow the rules below to help
ensure that your review can be posted.
Reviews by Our Customers Under the Age of 13
We highly value and respect everyone's opinion concerning the titles we offer.
However, we cannot allow persons under the age of 13 to have accounts at BN.com or
to post customer reviews. Please see our Terms of Use for more details.
What to exclude from your review:
Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the
information on the product page, please send us an email.
Reviews should not contain any of the following:
- HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
- Time-sensitive information such as tour dates, signings, lectures, etc.
- Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
- Comments focusing on the author or that may ruin the ending for others
- Phone numbers, addresses, URLs
- Pricing and availability information or alternative ordering information
- Advertisements or commercial solicitation
Reminder:
- By submitting a review, you grant to Barnes & Noble.com and its
sublicensees the royalty-free, perpetual, irrevocable right and license to use the
review in accordance with the Barnes & Noble.com Terms of Use.
- Barnes & Noble.com reserves the right not to post any review -- particularly
those that do not follow the terms and conditions of these Rules. Barnes & Noble.com
also reserves the right to remove any review at any time without notice.
- See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend
Create a Pen Name
Welcome, penname
You have successfully created your Pen Name. Start enjoying the benefits of the BN.com Community today.
If you find inappropriate content, please report it to Barnes & Noble
More About This Textbook
Overview
The diverse applications in this volume range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. Highlights include methods for heterologous expression of ion channels in cells, for determining channel structure-function, and for studying channel regulation.
Product Details
Related Subjects
Table of Contents
Part I. Methods for Exogenous Expression of Ion Channels in Cells
Functional Reconstitution of the Human Epithelial Na+ Channel in a Mammalian Expression System
Alexander Staruschenko, Rachell E. Booth, Oleh Pochynyuk, James D. Skand, and Qiusheng Tong
Overexpression of Proteins in Neurons Using Replication-Deficient Virus
Richard M. Ahlquist and Jane M. Sullivan
Exogenous Expression of Proteins in Neurons Using the Biolistic Particle Delivery System
Nikita Gamper and Mark S. Shapiro
Part II. Methods for Studying Channel Structure-Function
Tertiary and Quaternary Structure Formation of Voltage-Gated Potassium Channels
John M. Robinson, Andrey Kosolapov, and Carol Deutsch
Biophysical Approach to Determine the Subunit Stoichiometry of the Epithelial Sodium Channel Using the Xenopus laevis Oocyte Expression System
Farhad Kosari, Shaohu Sheng, and Thomas R. Kleyman
Spectroscopy-Based Quantitative Fluorescence Resonance Energy Transfer Analysis
Jie Zheng
Part III. Methods for Studying Channel Regulation and Physiological Function
Probing the Effects of Phosphoinositides on Ion Channels
Chou-Long Huang
Epithelial Sodium Channel in Planar Lipid Bilayers
Bakhrom K. Berdiev and Dale J. Benos
A Simple In Vivo Method for Assessing Changes of Membrane- Bound Ion Channel Density in Xenopus Oocytes
Mouhamed S. Awayda, Weijian Shao, Ivana Vukojicic, and Abderrahmane Bengrine
Preparation of Cortical Brain Slices for Electrophysiological Recording
Costa M. Colbert
Juxtacellular Labeling and Chemical Phenotyping of Extracellularly Recorded Neurons In Vivo
Glenn M. Toney and Lynette C. Daws
Carbon Fiber Amperometry in the Study of Ion Channels and Secretion
Duk-Su Koh
Part IV. Methods for Studying Channelopathies, Genetic Screening, and Molecular Biology
Genetic Screening for Functionality of Bacterial Potassium Channel Mutants Using K+ Uptake-Deficient Escherichia coli
Lyubov V. Parfenova and Brad S. Rothberg
KCNQ1 K+ Channel-Mediated Cardiac Channelopathies
Gildas Loussouarn, Isabelle Baró, and Denis Escande
Tissue-Specific Transgenic and Knockout Mice
Andrée Porret, Anne-Marie Mérillat, Sabrina Guichard, Friedrich Beermann, and Edith Hummler
Index