Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis / Edition 2

Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis / Edition 2

by Robert R. Wolfe, David L. Chinkes
     
 

ISBN-10: 0471462098

ISBN-13: 9780471462095

Pub. Date: 10/08/2004

Publisher: Wiley

This book offers the most thorough practical and theoretical treatment of tracer methodology as applied to the study of metabolic processes. It integrates biochemical, physiological, mathematical, and analytical aspects, with a focus on radioactive and stable isotopes in biomedical research. The authors systematically present complete information on how to perform

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Overview

This book offers the most thorough practical and theoretical treatment of tracer methodology as applied to the study of metabolic processes. It integrates biochemical, physiological, mathematical, and analytical aspects, with a focus on radioactive and stable isotopes in biomedical research. The authors systematically present complete information on how to perform these studies, from tracer selection, modeling considerations, sample derivitization, mass spectrometry analysis, and data interpretation. Problems and discussion questions have been added to highlight key points in each chapter. The contents have been fully updated and reorganized to enhance its utility, with notable expansion of coverage on protein metabolism and a brand new chapter on nuclear magnetic resonance (NMR).

Product Details

ISBN-13:
9780471462095
Publisher:
Wiley
Publication date:
10/08/2004
Edition description:
REV
Pages:
488
Sales rank:
1,116,678
Product dimensions:
7.19(w) x 10.00(h) x 1.12(d)

Table of Contents

Preface.

Chapter 1. Basic Characteristics of Isotopic Tracers.

Chapter 2. Calculating Specific Activity and Radiation Dosages.

Chapter 3. Calculation of Substance Kinetics: Single-Pool Model.

Chapter 4. Calculation of Substrate Kinetics: Multiple-Pool Model.

Chapter 5. Mass Spectrometry: Instrumentation.

Chapter 6. Determination of Isotopic Enrichment.

Chapter 7. Measurement of Substrate Oxidation.

Chapter 8. Measurement of Total Energy Expenditure Using the Doubly Labeled Water Method.

Chapter 9. Mass Isotopomer Distribution Analysis.

Chapter 10. Glucose Metabolism.

Chapter 11. Lipid Kinetics.

Chapter 12. Whole Body Protein Synthesis and Breakdown.

Chapter 13. Measurement of the Synthesis of Specific Proteins.

Chapter 14. Measurement of Regional or Tissue Protein Breakdown.

Chapter 15. Arterial-Venous Balance Technique to Measure Amino Acid Kinetics.

Chapter 16. Nuclear Magnetic Resonance.

Glossary.

Abbreviations.

Index.

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