Kinetic Analysis of Macromolecules: A Practical Approach

Hardcover (Print)
Used and New from Other Sellers
Used and New from Other Sellers
from $60.00
Usually ships in 1-2 business days
(Save 76%)
Other sellers (Hardcover)
  • All (3) from $60.00   
  • New (2) from $128.17   
  • Used (1) from $60.00   
Sort by
Page 1 of 1
Showing All
Note: Marketplace items are not eligible for any coupons and promotions
Seller since 2008

Feedback rating:



New — never opened or used in original packaging.

Like New — packaging may have been opened. A "Like New" item is suitable to give as a gift.

Very Good — may have minor signs of wear on packaging but item works perfectly and has no damage.

Good — item is in good condition but packaging may have signs of shelf wear/aging or torn packaging. All specific defects should be noted in the Comments section associated with each item.

Acceptable — item is in working order but may show signs of wear such as scratches or torn packaging. All specific defects should be noted in the Comments section associated with each item.

Used — An item that has been opened and may show signs of wear. All specific defects should be noted in the Comments section associated with each item.

Refurbished — A used item that has been renewed or updated and verified to be in proper working condition. Not necessarily completed by the original manufacturer.


Ships from: Chicago, IL

Usually ships in 1-2 business days

  • Standard, 48 States
  • Standard (AK, HI)
Seller since 2007

Feedback rating:


Condition: New
Brand New Item. International Shipping Available

Ships from: Dover, NJ

Usually ships in 1-2 business days

  • Standard, 48 States
Page 1 of 1
Showing All
Sort by


This new book in the Practical Approach Series contains reviews of state-of-the art kinetic methods applied to the functions and dynamics of proteins and nucleic acids. The book has been written by a team of experts, many of whom developed the methods discussed.

Read More Show Less

Editorial Reviews

Doody's Review Service
Reviewer: Eugene A Davidson, PhD (Georgetown University School of Medicine)
Description: This is a collection of articles/protocols covering detailed kinetic analysis of macromolecular interactions. The main thrust is to provide technical directions for kinetic analysis of enzymatic reactions.
Purpose: This book introduces the reader to kinetic analyses beyond the customary steady state measurements usually employed. In so doing, the opportunity to define mechanisms as well as rate determining steps is made clear. In terms of a more thorough analysis of enzyme reactions, this is worthwhile. Although each of the articles uses a specific reaction type for analysis, general principles can nonetheless be learned. In that sense, the goal of the book is achieved.
Audience: It is intended for the enzyme mechanist or drug developer with a strong background in conventional kinetics. Graduate students working in this area, postdoctoral fellows and laboratory directors will find material of value. The editor has assembled a qualified set of authors.
Features: Since the introduction of Michaelis-Menten kinetics (and subsequent refinements) beginning about 90 years ago, most kinetic analysis of enzymatic reactions has been dominated by that approach. Few investigators go beyond determination of the classic constants, Km and kcat. More recently, those studying details of enzyme mechanisms have employed isotopes such as deuterium or oxygen-18 to identify rate-determining steps or the origin of a specific oxygen. This book extends such analyses to the realm of transient kinetics wherein analysis of early stages of reactions (usually in the millisecond range) can provide information about possible intermediates (or rule out some) and therefore valuable information about the reaction path. Approaching the problem from a kinetic perspective, the opening chapter provides a valuable overview of the reasons for this type of approach. The remaining chapters offer protocols for various types of enzymatic reactions. Included is a chapter on the use of a modified enzyme as an analytical sensor (for inorganic phosphate) and one on enzymes that have specific residues replaced. Although each of the associated protocols deals with the specific system under study (ribozyme cleavage, DNA-protein interactions etc.), general principles are clearly there. Application to aspects of drug development is clearly delineated in the section on quench methodology (focus is on reactions of phosphoenolpyruvate). The investigator wishing to identify possible intermediates in a reaction pathway will find much useful information. This book can also be recommended to students since it will introduce them to the next level of analysis — something of value for the next generation of enzymologists. Regrettably, the title is somewhat obscure and could be better edited to read "Kinetic Analysis of Macromolecular Interactions."
Assessment: In spite of a somewhat distracting title, this is a useful book. The technical requirements for the work described may, on occasion, be formidable, but the information gained cannot be easily acquired otherwise. A useful contribution.

3 Stars from Doody
Read More Show Less

Product Details

  • ISBN-13: 9780198524939
  • Publisher: Oxford University Press, USA
  • Publication date: 12/28/2003
  • Series: Practical Approach Series, #267
  • Pages: 280
  • Product dimensions: 9.80 (w) x 7.70 (h) x 0.80 (d)

Meet the Author

The University of Texas at Austin
Read More Show Less

Table of Contents

List of protocols
List of contributors
Introduction to kinetic analysis of enzyme systems 1
Detection and characterization of enzyme intermediates: utility of rapid chemical quench methodology and single enzyme turnover experiments 19
Kinetic analysis of ribozyme cleavage 49
Kinetics analysis of DNA-protein interactions by time-resolved synchrotron X-ray footprinting 75
Transient-state kinetics and computational analysis of transcription initiation 87
A fluorescent sensor to assay inorganic phosphate 131
What to do if there is no signal: using competition experiments to determine binding parameters 153
The biotin regulatory system - kinetic, thermodynamic, and structural interrogation of a biological switch 171
Time resolved spectral analysis 203
Kinetic analysis of enzyme mutants for identification of catalytic residues 229
List of Suppliers 251
Index 255
Read More Show Less

Customer Reviews

Be the first to write a review
( 0 )
Rating Distribution

5 Star


4 Star


3 Star


2 Star


1 Star


Your Rating:

Your Name: Create a Pen Name or

Barnes & Review Rules

Our reader reviews allow you to share your comments on titles you liked, or didn't, with others. By submitting an online review, you are representing to Barnes & that all information contained in your review is original and accurate in all respects, and that the submission of such content by you and the posting of such content by Barnes & does not and will not violate the rights of any third party. Please follow the rules below to help ensure that your review can be posted.

Reviews by Our Customers Under the Age of 13

We highly value and respect everyone's opinion concerning the titles we offer. However, we cannot allow persons under the age of 13 to have accounts at or to post customer reviews. Please see our Terms of Use for more details.

What to exclude from your review:

Please do not write about reviews, commentary, or information posted on the product page. If you see any errors in the information on the product page, please send us an email.

Reviews should not contain any of the following:

  • - HTML tags, profanity, obscenities, vulgarities, or comments that defame anyone
  • - Time-sensitive information such as tour dates, signings, lectures, etc.
  • - Single-word reviews. Other people will read your review to discover why you liked or didn't like the title. Be descriptive.
  • - Comments focusing on the author or that may ruin the ending for others
  • - Phone numbers, addresses, URLs
  • - Pricing and availability information or alternative ordering information
  • - Advertisements or commercial solicitation


  • - By submitting a review, you grant to Barnes & and its sublicensees the royalty-free, perpetual, irrevocable right and license to use the review in accordance with the Barnes & Terms of Use.
  • - Barnes & reserves the right not to post any review -- particularly those that do not follow the terms and conditions of these Rules. Barnes & also reserves the right to remove any review at any time without notice.
  • - See Terms of Use for other conditions and disclaimers.
Search for Products You'd Like to Recommend

Recommend other products that relate to your review. Just search for them below and share!

Create a Pen Name

Your Pen Name is your unique identity on It will appear on the reviews you write and other website activities. Your Pen Name cannot be edited, changed or deleted once submitted.

Your Pen Name can be any combination of alphanumeric characters (plus - and _), and must be at least two characters long.

Continue Anonymously

    If you find inappropriate content, please report it to Barnes & Noble
    Why is this product inappropriate?
    Comments (optional)